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Journal of Central South University

, Volume 18, Issue 2, pp 386–391 | Cite as

Phenotypic and genetic characterization of a novel strain of Acidithiobacillus ferrooxidans (AF2)

  • Qian Liu (刘倩)
  • Hong-bo Zhou (周洪波)
  • Bo Yang (杨波)
  • Jing-qun Ao (敖敬群)
  • Xin-hua Chen (陈新华)Email author
Article

Abstract

A comparative study on the phenotypic and genetic characteristics among Acidithiobacillus ferrooxidans (AF2), a typic strain ATCC23270 and a previously isolated strain AF3 was performed. AF2 can use ferrous ion (Fe2+) or elemental sulfur (S0) as sole energy source, but oxidizes S0 more effectively than Fe2+, which is different from ATCC23270 and AF3. The G+C content of AF2 is 51.8% (molar fraction), however, ATCC23270 and AF3 strains have G+C content of 63.7% and 64.8% (molar fraction), respectively. The DNA-DNA hybridization results show that AF2 has 41.53% and 52.38% genome similarity to ATCC 23270 and AF3, respectively, but AF3 has a high genome similarity of 89.86% to ATCC 23270 strain. Rusticyanin (rus) and subunit III of aa3-type cytochrome oxidase (coxC) genes are not detected in AF2, but Fe2+ oxidase (iro) gene can be detected. To understand the genomic organization of iro gene, a cosmid library of AF2 genome was constructed and iro gene-containing clone was screened. The sequencing result shows that although the nucleotide sequence of iro gene in AF2 is completely identical to that of ATCC 23270 strain, its genomic organization is different from that of ATCC 23270. In AF2, iro is located at downstream of purA gene, while it is located at downstream of petC-2 gene in ATCC 23270 strain. These results indicate that AF2 is a novel strain of A. ferrooxidans, and that phenotypic differences among the strains of A. ferrooxidans are closely correlated with their genetic polymorphisms.

Key words

Acidithiobacillus ferrooxidans phenotypic and genetic characterization Iro gene cosmid library iron respiratory chain 

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References

  1. [1]
    YAMANAKA T, FUKUMORI Y. Molecular aspects of the electron transfer system which participates in the oxidation of ferrous ion by Thiobacillus ferrooxidans [J]. FEMS Microbiol Rev, 1995, 17(4): 401–413.CrossRefGoogle Scholar
  2. [2]
    CHEN Hong, YANG Bo, CHEN Xin-hua. Identification and characterization of four strains of Acidithiobacillus ferrooxidans isolated from different sites in China [J]. Microbiol Res, 2009, 164(6): 613–629.CrossRefGoogle Scholar
  3. [3]
    THOMPSON F L, HOSTE B, VANDEMEULEBROECKE K, SWINGS J. Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism [J]. Syst Appl Microbiol, 2001, 24(4): 520–538.CrossRefGoogle Scholar
  4. [4]
    KARAVAIKO G I, TUROVA T P, KONDRAT’EVA T F, LYSENKO A M, KOLGANOVA T V, AGEEVA S N, MUNTYAN L N, TAT’YANA A P. Phylogenetic heterogeneity of the species Acidithiobacillus ferrooxidans [J]. Int J Syst Evol Micr, 2003, 53(10): 113–119.CrossRefGoogle Scholar
  5. [5]
    RAWLINGS D E, KUSANO T. Molecular genetics of Thiobacillus ferrooxidans [J]. Microbiol Rev, 1994, 58(1): 39–55.Google Scholar
  6. [6]
    LANE D J, STAHL D A, OLSEN G J, HELLER D J, PACE N R. Phylogenetic analysis of the genera Thiobacillus and Thiomicrospira by 5S rRNA sequences [J]. J Bacteriol, 1985, 163(1): 75–81.Google Scholar
  7. [7]
    HUANG P C, ROSENBERG E. Determination of DNA base composition via depurination [J]. Anal Biochem, 1966, 16(1): 107–113CrossRefGoogle Scholar
  8. [8]
    MESBAH M, PREMACHANDRAN U, WILLIAM B W. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography [J]. Int J Syst Bacteriol, 1989, 39(10): 159–167.CrossRefGoogle Scholar
  9. [9]
    ANDO T, MONROE S S, GENTSCH J R, JIN Q, LEWIS D C, GLASS R I. Detection and differentiation of antigenically distinct small round-structured viruses (Norwalk-like viruses) by reverse transcription-PCR and southern hybridization [J]. Clin Microbiol, 1995, 33(1): 64–71.Google Scholar
  10. [10]
    GELLEN L S, WALL-MANNING G M, SISSONS C H. Checkerboard DNA-DNA hybridization technology using digoxigenin detection [J]. Methods Mol Biol, 2007, 353(5): 39–67.Google Scholar
  11. [11]
    WILLEMS A, DOIGNON-BOURCIER F, GORIS J, COOPMAN R, DE LAJUDIE P, DEVOS P, GILLIS M. DNA-DNA hybridization study of Bradyrhizobium strains [J]. Int J Syst Evol Micr, 2001, 51(4): 1315–1322.CrossRefGoogle Scholar
  12. [12]
    JORN P. A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles [J]. Proc Natl Acad Sci, 2002, 99: 14002–14007.CrossRefGoogle Scholar
  13. [13]
    NORRIS P R, CLARK D A, OWEN J P, WATERHOUSE S. Characteristics of sulfobacillus acidophilus sp. nov. and other moderately thermophilic mineral-sulphide-oxidizing bacteria [J]. Microbiol, 1996, 142: 775–783.CrossRefGoogle Scholar
  14. [14]
    RAWLINGS D E. Characteristics and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates [J]. Microb Cell Fact, 2005, 4(13): 1–15.Google Scholar
  15. [15]
    AGEEVA S N, KONDRAT’EVA T F, KARAVAIKO G I. Phenotypic characteristics of Thiobacillus ferrooxidans strains [J]. Microbiol, 2001, 70(2): 186–194CrossRefGoogle Scholar
  16. [16]
    HARRISON A P. Genomic and physiological diversity amongst strains of Thiobacillus ferrooxidans and genomic comparison with Thiobacillus thiooxidans [J]. Arch Microbiol, 1982, 131(10): 68–76.CrossRefGoogle Scholar
  17. [17]
    MOSTAFA M, USHA P, WILLIAM B. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography [J]. Int J Syst Bacteriol, 1989, 39(2): 159–167.CrossRefGoogle Scholar
  18. [18]
    BRUSCELLA P, APPIA-AYME C, LEVICAN G, RATOUCHNIAK J, JEDLICKI E, HOLMES D S, BONNEFOY V. Differential expression of two bc1 complexes in the strict acidophilic chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans suggests a model for their respective roles in iron or sulfur oxidation [J]. Microbiol, 2007, 153(10): 102–110.CrossRefGoogle Scholar
  19. [19]
    BRUSCELLA P, CASSAGNAUD L, RATOUCHNIAK J, BRASSEUR G, LOJOU E, AMILS R, BONNEFOY V. The HiPIP from the acidophilic Acidithiobacillus ferrooxidans is correctly processed and translocated in Escherichia coli, in spite of the periplasm pH difference between these two micro-organisms [J]. Microbiol, 2005, 151(10): 1421–1431.CrossRefGoogle Scholar
  20. [20]
    APPIA-AYME C, GUILIANI N, RATOUCHNIAK J, BONNEFOY V. Characterization of an operon encoding two c-type cytochromes, an aa(3)-type cytochrome oxidase, and rusticyanin in Thiobacillus ferrooxidans ATCC 33020 [J]. Appl Environ Microb, 1999, 65(11): 4781–4787.Google Scholar
  21. [21]
    YARZABAL A, APPIA-AYME C, RATOUCHNIAK J, BONNEFOY V. Regulation of the expression of the Acidithiobacillus ferrooxidans rus operon encoding two cytochromes c, a cytochrome oxidase and rusticyanin [J]. Microbiol, 2004, 150(7): 2113–2123.CrossRefGoogle Scholar
  22. [22]
    COX J C, BOXER D H. The purification and some properties of rusticyanin, a blue copper protein involved in iron(II) oxidation from Thiobacillus ferrooxidans [J]. Biochem J, 1978, 174(12): 497–502.Google Scholar
  23. [23]
    WANG J, WANG Y J, GUO H. Ballistic-electron transport through a coupled-quantum-wire system [J]. Phys Rev B: Condens Matter, 1992, 46(4): 2420–2427.CrossRefGoogle Scholar

Copyright information

© Central South University Press and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Qian Liu (刘倩)
    • 1
    • 2
  • Hong-bo Zhou (周洪波)
    • 1
  • Bo Yang (杨波)
    • 2
  • Jing-qun Ao (敖敬群)
    • 2
  • Xin-hua Chen (陈新华)
    • 2
    Email author
  1. 1.School of Minerals Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.Key Laboratory of Marine Biogenetic ResourcesThird Institute of Oceanography, State Oceanic AdministrationXiamenChina

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