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Journal of Central South University of Technology

, Volume 16, Issue 6, pp 948–953 | Cite as

Aerobic biodegradation of di-n-butyl phthalate by Xiangjiang River sediment and microflora analysis

  • Hong-bo Zhou (周洪波)Email author
  • Feng Lin (林 峰)
  • Pei-lei Hu (胡培磊)
  • De-cai Jing (金德才)
  • Hong-qiang Ren (任洪强)
  • Jing Zhao (赵 晶)
  • Guan-zhou Qiu (邱冠周)
Article

Abstract

Di-n-butyl phthalate (DBP), one of phthalate acid esters (PAEs), was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the sediment. The sediment sample was incubated with an initial concentration of DBP of 100 mg/L for 5 d. The biodegradation rate of DBP was detected using HPLC and the degraded products were analyzed by GC/MS. Subsequently, the microbial diversity of the enrichment culture was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results reveal that almost 100% of DBP is degraded after merely 3 d, generating two main degraded products: mono-butyl phthalate (MBP) and 9-octadecenoic acid. After a six-month enrichment period under the pressure of DBP, the dominant family in the final enrichment culture is clustered with the Comamonas sp., the remaining are affiliated with Sphingomonas sp., Hydrogenophaga sp., Rhizobium sp., and Acidovorax sp. The results show the potential of these bacteria to be used in the bioremediation of DBP in the environment.

Key words

bioremediation di-n-butyl phthalate sediment polymerase chain reaction-restriction fragment length polymorphism microbial diversity 

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Copyright information

© Central South University Press and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Hong-bo Zhou (周洪波)
    • 1
    Email author
  • Feng Lin (林 峰)
    • 1
  • Pei-lei Hu (胡培磊)
    • 1
  • De-cai Jing (金德才)
    • 1
  • Hong-qiang Ren (任洪强)
    • 2
  • Jing Zhao (赵 晶)
    • 3
  • Guan-zhou Qiu (邱冠周)
    • 1
  1. 1.School of Minerals Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.State Key Laboratory of Pollution Control and Resources ReuseNanjing UniversityNanjingChina
  3. 3.School of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanChina

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