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Comparative analysis of chemical components between barks and leaves of Eucommia ulmoides Oliver

  • Ju-feng Zhou (周菊峰)
  • Tai-ming Zhang (张泰铭)Email author
  • Wang-ai Chen (陈望爱)
  • Yi-zeng Liang (梁逸曾)
Article

Abstract

The chemical components of the essential oils in the barks and leaves of Eucommia ulmoides Oliver were analyzed and compared by chromatograms and mass spectra technique, heuristic evolving latent projections (HELP), alternative moving window factor analysis (AMWFA) algorithms and normalization method based on the peak areas; the flavones in the barks and leaves of Eucommia ulmoides Oliver were separated on an ODS column by gradient elution carried out with the flow phase consisting of water, methanol and phosphoric acid (0.1%), and their contents were quantitatively determined by standard curve method and diode array detection (DAD) at 362 nm. The results show that 68 and 73 compounds respectively from essential oils of the barks and leaves of Eucommia ulmoides Oliver are identified, and there are 33 mutual compounds among 108 compounds determined. The total contents of these volatile components of the two samples possess 92.9% and 97.75% of the gross of the relevant essential oils, respectively; the contents of the rutin, quercetin and kaempferol in the barks and leaves of Eucommia ulmoides Oliver are 0.016 9, 0.003 6, 0.002 1 and 0.064 4, 0.030 2, 0.010 0 mg/g, respectively, and the determination recoveries are 95.2%–106.2%. The comparative analysis shows that for the barks and leaves of Eucommia ulmoides Oliver, there are significant differences in their components of the relevant essential oils and flavones.

Key words

couple chromatography chemometric algorithm comparative analysis essential oil flavone Eucommia ulmoides Oliver 

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Copyright information

© Central South University Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Ju-feng Zhou (周菊峰)
    • 1
    • 2
  • Tai-ming Zhang (张泰铭)
    • 1
    Email author
  • Wang-ai Chen (陈望爱)
    • 1
  • Yi-zeng Liang (梁逸曾)
    • 1
  1. 1.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina
  2. 2.Department of Chemistry and Life ScienceXiangnan UniversityChenzhouChina

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