Bioleaching of chalcocite by mixed microorganisms subjected to mutation

  • Jian Kang (康 健)
  • Guan-zhou Qiu (邱冠周)
  • Jian Gao (高健)
  • Hai-hua Wang (王海华)
  • Xue-ling Wu (吴学玲)
  • Jian-nan Ding (丁建南)
Article

Abstract

Mixed microorganisms with elevated activity of chalcocite-leaching were screened by mutation methods. The original microorganisms collected from acid mine drainage of different sites were mixed and then treated with mutagens NO2, diethyl sulfate (DES), UV and their combinations, respectively. Five groups of mixed microorganisms with much stronger ore-leaching ability were obtained by screening on the leaching media. Among them, group E of mixed microorganisms (treated with 1% DES for 60 min) with the best performance on chalcocite-leaching, increases the content of Cu2+ by 101.4% in 20 d of leaching compared with the control culture. In addition, group E is more tolerant to Cu2+ in media than the control without mutation treatment. Analysis for the diversity of microbial clones indicates that half of operational taxonomic units (OTUs) in group E are Acidithiobacillus ferrooxidans. These observations suggest that group E might have potentials for industrial application.

Key words

chalcocite mixed microorganisms bioleaching mutation 

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Copyright information

© Central South University Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Jian Kang (康 健)
    • 1
    • 2
  • Guan-zhou Qiu (邱冠周)
    • 1
  • Jian Gao (高健)
    • 2
  • Hai-hua Wang (王海华)
    • 2
  • Xue-ling Wu (吴学玲)
    • 1
  • Jian-nan Ding (丁建南)
    • 1
  1. 1.School of Resources Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.School of Life ScienceHunan University of Science and TechnologyXiangtanChina

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