Bioleaching of chalcocite by mixed microorganisms subjected to mutation
- 81 Downloads
Mixed microorganisms with elevated activity of chalcocite-leaching were screened by mutation methods. The original microorganisms collected from acid mine drainage of different sites were mixed and then treated with mutagens NO2−, diethyl sulfate (DES), UV and their combinations, respectively. Five groups of mixed microorganisms with much stronger ore-leaching ability were obtained by screening on the leaching media. Among them, group E of mixed microorganisms (treated with 1% DES for 60 min) with the best performance on chalcocite-leaching, increases the content of Cu2+ by 101.4% in 20 d of leaching compared with the control culture. In addition, group E is more tolerant to Cu2+ in media than the control without mutation treatment. Analysis for the diversity of microbial clones indicates that half of operational taxonomic units (OTUs) in group E are Acidithiobacillus ferrooxidans. These observations suggest that group E might have potentials for industrial application.
Key wordschalcocite mixed microorganisms bioleaching mutation
Unable to display preview. Download preview PDF.
- IZAMA H M, SUZUKI I J. Bacterial leaching of a sulphide ore by Thiobacillus ferrooxidans and Thiobacillus thiooxidans shake flask studies [J]. Biotechology and Bioengineering, 1988, 22: 110–116.Google Scholar
- LI H X, WANG D Z. Review of investigation on microorganism behaviors in ore bioleaching [J]. Nonferrous Metals, 2003, 55(2): 59–63.Google Scholar
- NORRIS P R, MARSH R M, LINSTROM E B. Growth of mesophilic and thermophilic acidophilic baeteria on sulfur and tetrathionate biotechnology [J]. Applied Biochemistry, 1986, 8: 318–329.Google Scholar
- KONIG H, SKORKO R J. ZILLIG W, REITER W. D. Glycogen in Thermoacidophilic archaebacteria of the genera sulfolobus, thermoproteus, desulfurococcus and thermococcus [J]. Achives of Microbiology, 1982, 132(4): 297–303.Google Scholar
- KANG Jian, GAO Jian, WU Xue-lin, DING Jian-nan, QIU Guan-zhou. Mutagenesis of mixed bacteria and influence on bioleaching of sphalerite with the mutagenized bacterial admixture [J]. Journal of Central South University: Science and Technology, 2007, 38(3): 439–444. (in Chinese)Google Scholar
- KANG Jian, DING Jian-nan, GAO Jian, WU Xue-lin, QIU Guan-zhou. Study on growth of mixed bacteria on zinc ore after mutation [J]. Progress in Modern Biomedicine, 2007, 7(4): 489–493. (in Chinese)Google Scholar
- SILVERMAN M P, LUNDGREN D J. Studies on the chemoautotrophic iron bacterium Thiobacillus ferrooxidans (I): An improved medium and a harvesting procedure for securing high cellular yields [J]. Journal of Bacteriology, 1959, 77(5): 642–647.Google Scholar
- KARAVAIKO G I, KARAVAIKO G I, ROSSI G, AGATE A D, GROUDEV S, AVAKYAN Z. Biogeotechnology of metals-manual [M]. Moscow: Centre for International Projects GKNT, 1988: 59–61.Google Scholar
- OGRAM A. Discussion soil molecular microbial ecology at age 20: Methodological challenges for the future [J]. Soil Biology and Biochemistry, 2000, 32(11/12): l499–l504.Google Scholar
- MA W L, JOSQUIN T, MARK A. A new method for research on soil microbial diversity [J]. Acta Pedologica Sinica, 2004, 41(1): 103–107.Google Scholar