Bioleaching of chalcocite by mixed microorganisms subjected to mutation

  • Jian Kang (康 健)
  • Guan-zhou Qiu (邱冠周)Email author
  • Jian Gao (高健)
  • Hai-hua Wang (王海华)
  • Xue-ling Wu (吴学玲)
  • Jian-nan Ding (丁建南)


Mixed microorganisms with elevated activity of chalcocite-leaching were screened by mutation methods. The original microorganisms collected from acid mine drainage of different sites were mixed and then treated with mutagens NO2, diethyl sulfate (DES), UV and their combinations, respectively. Five groups of mixed microorganisms with much stronger ore-leaching ability were obtained by screening on the leaching media. Among them, group E of mixed microorganisms (treated with 1% DES for 60 min) with the best performance on chalcocite-leaching, increases the content of Cu2+ by 101.4% in 20 d of leaching compared with the control culture. In addition, group E is more tolerant to Cu2+ in media than the control without mutation treatment. Analysis for the diversity of microbial clones indicates that half of operational taxonomic units (OTUs) in group E are Acidithiobacillus ferrooxidans. These observations suggest that group E might have potentials for industrial application.

Key words

chalcocite mixed microorganisms bioleaching mutation 


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  1. [1]
    YASUHIRO K, SATORU A, MASAHIKO T, TORU S. Kinetics of the bioleaching of chalcopyrite concentrate by acidophilic thermophile Acidianus brierleyi [J]. Biotechnology Progress, 1999, 15(4): 681–688.CrossRefGoogle Scholar
  2. [2]
    HARRISON A P. Genomic and physiological diversity amongst strains of Thiobacillus ferrooxidans, and genomic comparison with Thiobacillus thiooxidans [J]. Archives of Microbiology, 1982, 131(1): 68–76.CrossRefGoogle Scholar
  3. [3]
    IZAMA H M, SUZUKI I J. Bacterial leaching of a sulphide ore by Thiobacillus ferrooxidans and Thiobacillus thiooxidans shake flask studies [J]. Biotechology and Bioengineering, 1988, 22: 110–116.Google Scholar
  4. [4]
    LI H X, WANG D Z. Review of investigation on microorganism behaviors in ore bioleaching [J]. Nonferrous Metals, 2003, 55(2): 59–63.Google Scholar
  5. [5]
    NORRIS P R, MARSH R M, LINSTROM E B. Growth of mesophilic and thermophilic acidophilic baeteria on sulfur and tetrathionate biotechnology [J]. Applied Biochemistry, 1986, 8: 318–329.Google Scholar
  6. [6]
    NORRIS P R, BARR D B. Growth and iron oxidation by moderate thermophiles [J]. FEMS Microbiology Letters, 1985, 28(3): 221–224.CrossRefGoogle Scholar
  7. [7]
    KONIG H, SKORKO R J. ZILLIG W, REITER W. D. Glycogen in Thermoacidophilic archaebacteria of the genera sulfolobus, thermoproteus, desulfurococcus and thermococcus [J]. Achives of Microbiology, 1982, 132(4): 297–303.Google Scholar
  8. [8]
    KANG Jian, GAO Jian, WU Xue-lin, DING Jian-nan, QIU Guan-zhou. Mutagenesis of mixed bacteria and influence on bioleaching of sphalerite with the mutagenized bacterial admixture [J]. Journal of Central South University: Science and Technology, 2007, 38(3): 439–444. (in Chinese)Google Scholar
  9. [9]
    KANG Jian, DING Jian-nan, GAO Jian, WU Xue-lin, QIU Guan-zhou. Study on growth of mixed bacteria on zinc ore after mutation [J]. Progress in Modern Biomedicine, 2007, 7(4): 489–493. (in Chinese)Google Scholar
  10. [10]
    SILVERMAN M P, LUNDGREN D J. Studies on the chemoautotrophic iron bacterium Thiobacillus ferrooxidans (I): An improved medium and a harvesting procedure for securing high cellular yields [J]. Journal of Bacteriology, 1959, 77(5): 642–647.Google Scholar
  11. [11]
    KARAVAIKO G I, KARAVAIKO G I, ROSSI G, AGATE A D, GROUDEV S, AVAKYAN Z. Biogeotechnology of metals-manual [M]. Moscow: Centre for International Projects GKNT, 1988: 59–61.Google Scholar
  12. [12]
    HORTON T R, BRUNS T D. The molecular revolution in ectomycorrhizal ecology: Peeking into the black-box [J]. Molecular Ecology, 2001, 10(8): 1855–1871.CrossRefGoogle Scholar
  13. [13]
    OGRAM A. Discussion soil molecular microbial ecology at age 20: Methodological challenges for the future [J]. Soil Biology and Biochemistry, 2000, 32(11/12): l499–l504.Google Scholar
  14. [14]
    BRIDGE P, BRIAN S. Soil fungi: Diversity and detection [J]. Plant and Soil, 2001, 232(1/2): 147–154.CrossRefGoogle Scholar
  15. [15]
    DUNBAR J, TICKNOR L O, KUSKE C R I. Assessment of microbial diversity in four Southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis [J]. Applied and Environmental Microbiology, 2000, 66(7): 2943–2950.CrossRefGoogle Scholar
  16. [16]
    MA W L, JOSQUIN T, MARK A. A new method for research on soil microbial diversity [J]. Acta Pedologica Sinica, 2004, 41(1): 103–107.Google Scholar

Copyright information

© Central South University Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Jian Kang (康 健)
    • 1
    • 2
  • Guan-zhou Qiu (邱冠周)
    • 1
    Email author
  • Jian Gao (高健)
    • 2
  • Hai-hua Wang (王海华)
    • 2
  • Xue-ling Wu (吴学玲)
    • 1
  • Jian-nan Ding (丁建南)
    • 1
  1. 1.School of Resources Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.School of Life ScienceHunan University of Science and TechnologyXiangtanChina

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