Bioleaching of sphalerite by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans cultured in 9K medium modified with pyrrhotite
- 202 Downloads
Elective culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in 9K medium modified with pyrrhotite was studied. Bioleaching of flotation concentrate of sphalerite by the selected bacteria was carried out. The results show that the microorganisms cultured by pyrrhotite are a mixture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, of which the capability to oxidize ferrous to ferric irons is enhanced by the high mass ratio of Fe to S in pyrrhotite. Three pyrrhotite samples were separated into various parts with corresponding S/Fe ratios by magnetic separation and were used to culture the elective bacteria as the substrate. The association of the cultures could provide a more rapid and complete oxidation of sphalerite than that of bacteria cultivated by conventional methods.
Key wordsbioleaching pyrrhotite elective culture sphalerite
Unable to display preview. Download preview PDF.
- MILLER P C, RHODES M K, WINBY R. Commercialization of bioleaching for base-metal extraction [J]. Minerals & Metallurgical Processing, 1999, 16(4): 42–50.Google Scholar
- SAND W, RHODE K, SOBOTKE B. Evaluation of Leptospirillum ferrooxidants for leaching [J]. Appl Environ Microbiol, 1992, 58(1): 85–92.Google Scholar
- LUO Lian-ming, WANG Jun, XU Jing. Study on methods of improving bacterial leaching velocity of gold ores [J]. Conservation and Utilization of Mineral Resources, 1994(4): 40–43. (in Chinese)Google Scholar
- LIANG Dong-yun, HE Guo-wei, ZOU Ni. The isomeromorphism of pyrrhotite and its treatment feature differentia [J]. Journal of Guangdong Nonferrous Metals, 1997, 7(1): 1–5. (in Chinese)Google Scholar
- JI S, SHANG Z C. The determination of Zn2+ in compound of Zn2+ and Fe2+ by EDTA titration [J]. Shandong Chem Ind, 2000, 29: 40–41. (in Chinese)Google Scholar
- LI Hong-mei. Bioleaching of pyrrhotite bearing nickel—A review [J]. Hydrometallurgy, 1999(3): 8–12. (in Chinese)Google Scholar
- FOWLER T A, CRUNDWELL F K. Leaching of zinc sulfide by thiobacillus ferrooxidans: Experiments with a controlled redox potential indicate no direct bacterial mechanism [J]. Appl Environ Microbiol, 1998, 64(10): 3570–3575.Google Scholar
- FOWLER T A, CRUNDWELL F K. Leaching of zinc sulfide by thiobacillus ferrooxidans: Bacterial oxidation of the sulfur product layer increases the rate of zinc sulfide dissolution at high concentration of ferrous ions [J]. Appl Environ Microbiol, 1999, 65(12): 5285–5292.Google Scholar