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Determination of volatile components in cut tobacco with gas chromatography-mass spectrometry and chemometric resolution

  • Huang Lan-fang  (黄兰芳)Email author
  • Wu Ming-jian  (吴名剑)
  • Sun Xian-jun  (孙贤军)
  • Zhong Ke-jun  (钟科军)
  • Guo Zi-ming  (郭紫明)
  • Dai Yun-hui  (戴云辉)
  • Huang Ke-long  (黄可龙)
  • Guo Fang-qiu  (郭方遒)
Article

Abstract

Chromatography-mass spectrometry (GC-MS) was used to analyze the volatile components of cut tobacco samples with the help of heuristic evolving latent projections (HELP). After extracting with simultaneous distillation and extraction method, the volatile components in cut tobacco were detected by GC-MS. Then the obtained original two-dimensional data were resolved into pure mass spectra and chromatograms. The qualitative analysis was performed by similarity searches in the national institute of standards and technology (NIST) mass database with the obtained pure mass spectrum of each component and the quantitative results were obtained by calculating the volume of total two-way response. The accuracy of qualitative and quantitative results were greatly improved by using the two-dimensional comprehensive information of chromatograms and mass spectra. 107 of 141 separated constituents in the total ion chromatogram of the volatile components were identified and quantified, accounting for about 88.01% of the total content. The result proves that the developed method is powerful for the analysis of complex cut tobacco samples.

Key words

chemometrics gas chromatography-mass spectrometry heuristic evolving latent projection cut tobacco volatile component 

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Copyright information

© Central South University Press, Sole distributor outside Mainland China: Springer 2007

Authors and Affiliations

  • Huang Lan-fang  (黄兰芳)
    • 1
    • 2
    Email author
  • Wu Ming-jian  (吴名剑)
    • 1
    • 3
  • Sun Xian-jun  (孙贤军)
    • 1
  • Zhong Ke-jun  (钟科军)
    • 1
  • Guo Zi-ming  (郭紫明)
    • 1
  • Dai Yun-hui  (戴云辉)
    • 1
    • 2
  • Huang Ke-long  (黄可龙)
    • 2
  • Guo Fang-qiu  (郭方遒)
    • 2
  1. 1.Technical Center of Changde Cigarette FactoryChangdeChina
  2. 2.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina
  3. 3.College of ChemistryXiangtan UniversityXiangtanChina

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