Effects of low dose oxymatrine on mouse lymphocyte proliferation stimulated by Con A
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Objective To investigate the effects of low dose Oxymatrine (OMT) on mouse lymphocyte proliferation stimulated by Con A making use of fluorescence dyestuff (CFDA-SE). Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal stimulators Con A and OMT. Then, related software was used to analyze the effects of OMT on mouse lymphocyte proliferation. Results After cultured for 48 h, CFSE fluorescence could be detected by cytometer, filial generation peaks did not appear in control group, which indicated that lymphocytes did not proliferate. Three peaks were obviously detected in Con A group which indicated that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control group. In groups with Con A and OMT treated. Primary generation peaks are all lower while filial generation peaks are significantly higher than groups with Con A treated only. This indicated OMT obviously promote lymphocyte proliferation. After cultured for 72 h, the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h. Analyzed with CELLQuest software, it is shown that OMT could promote lymphocyte proliferation in 16, 8, 4 and 2 μg/mL respectively. Conclusions 1)CFDA-SE dyeing and flow cytometer were both reliable tools to analyse lymphocyte proliferation; 2) lower dosage of OMT could promote the proliferation of lymphocyte as a immunopotentiator.
Key wordsCFDA-SE OMT lymphocyte proliferation
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- MA Jun-jiang, CHEN Xue-rong, SI La-pu, et al. Restrain of oxymatrine on type I–IV allergic reactions [J]. Journal of Beijing Medical University, 1991, 23(6): 445.Google Scholar
- ZHAO Jing-xian, ZENG Yao-ying. Application of alive dyestuff CFDA-SE in the research of lymphocyte proliferation[J]. Journal of Cellular and Molecular Immunology, 2003, 19(2): 109–111.Google Scholar
- WANG Xiao, DONG Xiang-yu, ZHU Chuan-an. Effects of oxymatrine on peripheral T lymphocyte subsets of chronic HBV hepatitis patient[J]. Clinical Focus, 2001, 16 (18): 841.Google Scholar
- LI Zheng-rong. The progress of oxymatrine in pharmacology and cinical research[J]. West China Journal of Pharmaceutical Sciences, 2003, 18(6): 435–437.Google Scholar
- SHEN Zhi-hong, WU Yi-xuan, MAO Wei-han. Injection of oxymatrine treat all kinds of eczema[J]. Chin J New Drugs Clin Rem, 2000, 19(6): 473–474.Google Scholar
- HU Ya-ni, WANG Si-wang, XIE Yan-hua. The research of oxymatrine on antitumor effects[J]. Journal of Liaoning College of Traditional Chinese, 2003, 5 (1): 3–5.Google Scholar
- KONG Qing-zhi, HUANG Tao, HUANG Dong-sheng, et al. Experimental study on inhibiting effect of oxymatrine on mice's angiogenesis from S180 sarcoma [J]. China Pharmacist, 2003, 6(12): 769–771Google Scholar
- GAO Hong-quan, WANG Ying, YANG Chung-zhuang. Studies on oxymatrine induces apoptosis in ovarian cancer cell lines HO8910[J]. Acta Chinese Medicine and Pharmacology, 2004, 32(2: 52–55Google Scholar