Determination of constituents of essential oil from Angelica sinensis by gas chromatography — mass spectrometry

  • Wu Ming-jian 
  • Sun Xian-jun 
  • Dai Yuan-hui 
  • Guo Fang-qiu 
  • Huang Lan-fang 
  • Liang Yi-zeng 
Article
Received:
Accepted:

Abstract

Gas chromatography — mass spectrometry (GC-MS) coupled with chemometric resolution upon two-dimensional data was employed to analyze the constituents of essential oils of essential oils of Angelica sinensis. Constituents in essential oils of Angelica sinensis root were identified by GC-MS with the help of subwindow factor analysis (SFA) method resolving two-dimensional original data into mass spectra and chromatograms. 76 of 97 separated constituents in essential oil of Angelica sinensis root were identified and quantified, and they account for about 91. 36% of the total content. The results show that ligustilide, butylene phthalide, 2-methoxy-4-vinylphenol, carvacrol, alloocimene,2,6,6-trimethylbicyclo-[3,1,1]hept-2-ene are the main constituents in essential oil of Angelica sinensis root.

Key words

Angelica sinensis chemometrics gas chromatography — mass spectrometry subwindow factor analysis 

CLC number

O657.7+1 R284.1 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    Wang H, Zou H, Kong L, et al. Analysis of bioactive components in traditional Chinese medicines by molecular biochromatography with alpha1-acid glycoprotein stationary phase [J]. J Basic Clin Physiol Pharmacol, 2000, 11 (2): 155–172.Google Scholar
  2. [2]
    Liang Y Z. White, Gray and Black Multicomponent Systems and Their Chemometric Algorithms [M]. Changsha: Hunan Science and Technology Press, 1996. (in Chinese)Google Scholar
  3. [3]
    Gong F, Liang Y Z, Xu Q S, et al. Determination of volatile components in peptic powder by Gas chromatography-mass spectrometry and chemometric resolution [J]. J Chromatography A, 2001, 909(2): 237–247.CrossRefGoogle Scholar
  4. [4]
    Li X N, Cui H, Song Y Q, et al. Analysis of the volatile fraction of schisandra Chinensis (Turcz.) baill. with GC/MS and chemometric resolution [J]. Phytochemical Analysis, 2003, 14(1): 23–33.CrossRefGoogle Scholar
  5. [5]
    Keller H R, Massart D L. Peak purity control in liquid chromatography with photodiode-array detection by a fixed size moving window evolving factor analysis [J]. Anal Chim Acta, 1991, 246(4): 379–390.CrossRefGoogle Scholar
  6. [6]
    Maeder M, Zilian A. Evolving factor analysis, a new multivariate techniques in chromatography [J]. Chemom Intell Lab Syst, 1988, 3(3): 205–213.CrossRefGoogle Scholar
  7. [7]
    Maeder M, Zuberbuhler A D. The resolution of overlapping chromatographic peaks by evolving factor analysis [J]. Anal Chim Acta, 1986, 181(2): 287–291.CrossRefGoogle Scholar
  8. [8]
    Xu C J, Jiang J H, Liang Y Z. Evolving window orthogonal projections method for two-way data resolution [J]. Analyst, 1999, 124 (10): 1471–1476.CrossRefGoogle Scholar
  9. [9]
    Liang Y Z, Kvalheim O M, Keller H R, et al. Heuristic evolving latent projections: resolving two-way multicomponent data. Part 2: Detection and resolution of minor constituents [J]. Anal Chem, 1992, 64(8): 946–953.CrossRefGoogle Scholar
  10. [10]
    Manne R, Shen H L, Liang Y Z. Subwindow factor analysis [J]. Chemom Intell Lab Syst, 1999, 45(1–2): 171–176.CrossRefGoogle Scholar
  11. [11]
    Shen H L, Manne R, Xu Q S, et al. Local resolution of hyphenated chromatographic data [J]. Chemom Intell Lab Syst, 1999, 45(1–2): 323–330.CrossRefGoogle Scholar
  12. [12]
    CHENG Yao-zu, CHENG Neng-yi, MA Xue-yi, et al. Study on the constituents of Angelica sinensis [J]. Chemical Journal of Chinese University, 1984, 5(1): 125. (in Chinese)Google Scholar
  13. [13]
    CHENG Nan, XUE Dun-yuan. Study on the SC-CO2 extracted of the essential oil in Angelica Sinensis [J]. Journal of Gansu Educational College (Natural Sciences), 2003, 17(4): 30–33. (in Chinese)Google Scholar
  14. [14]
    LI Gui-sheng, MA Cheng-jun, LIU Zhi-feng, et al. Extraction of the essential oil from Angelica sinensis by supercritical-CO2 fluid in comparison with that with stream distillation [J]. Chinese Traditional and Herbal Drug, 2001, 32(7): 581–583. (in Chinese)Google Scholar
  15. [15]
    Chinese Pharmacopoeia Committee. Pharmacopoeia of the People’s Republic of China (English Edition) [M]. Guangzhou: Guangdong Science and Technology Press, 1988.Google Scholar
  16. [16]
    Chinese Pharmacopoeia Committee. Chinese Pharmacopoeia-Appendix 62 [M]. Beijing: People’s Health Press, 1995. (in Chinese)Google Scholar

Copyright information

© Central South University 2005

Authors and Affiliations

  • Wu Ming-jian 
    • 1
  • Sun Xian-jun 
    • 1
  • Dai Yuan-hui 
    • 1
  • Guo Fang-qiu 
    • 2
  • Huang Lan-fang 
    • 2
  • Liang Yi-zeng 
    • 2
  1. 1.Technical CenterChangde Cigarette FactoryChangdeChina
  2. 2.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

Personalised recommendations