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Microwave-assisted extraction of polysaccharides from solanum nigrum

  • Chen Xiao-qing Email author
  • Liu qin 
  • Jiang xin-yu 
  • Zeng fan 
Article

Abstract

The microwave-assisted method was used to extract polysaccharides from solanum nigrum. The optimum experimental parameters, mechanism of the extraction and the effect of microwave-assisted extraction process on the structures of polysaccharides were investigated. The extract was analyzed by the modified phenol-sulfuric acid method at 490 nm. The optimum experimental parameters were obtained by orthogonal experiments as follows: extraction time 15 min, microwave radiation power 455 W and the process ratio of materials mass to solvent volume 1:20. The results show that compared with the conventional reflux extraction, the microwave-assisted extraction has a higher yield in shorter time, with no effect on the finally obtained polysaccharides as seen from the FT-IR spectra. The scanning electron microscopy images reveal that the mechanism of the extraction is related to the structural changes of the plant cells in different extracting conditions.

Key words

solanum nigrum polysaccharides structure microwave-assisted extraction 

CLC number

R284.1 

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References

  1. [1]
    LU Cui-xia, LI Xiu-rong. The general study of clinic and experiment on the treatment on hepatic cellular carcinoma in middle or advanced stage with Chinese heat-clearing and detoxifying herb [J]. Journal of Shandong University of Traditional Chinese Medicine, 1999, 23(3): 224–227. (in Chinese)Google Scholar
  2. [2]
    DING Guo-dong, YANG Guang-wen, ZHANG Xiao-jian. The evaluation on the clinical efficacy of the treatment on hepatic carcinoma in middle or advanced stage with Dongshen injection[J]. Study and Clinical Application on Tumor, 1999, 11(2): 123–124. (in Chinese)Google Scholar
  3. [3]
    XIAO Gui-wu, ZENG He-pin. Isolation, purification and identification of polysaccharides from black night-shade(solanum nigrum) (II) [J]. Chinese Traditional and Herbal Drugs, 2000, 31(3): 162–164. (in Chinese)Google Scholar
  4. [4]
    XIAO Gui-wu, SHEN-Lan, ZENG He-pin. The analysis of the water-soluble polysaccharides from solanum nigrum L[J]. Chinese Medicinal Materials, 1999, 22(8): 406–406. (in Chinese)Google Scholar
  5. [5]
    XIAO Gui-wu, ZENG He-pin. Isolation, purification and identification of polysaccharides from solanum nigrum L [J]. Pharmaceutical Biotechnology, 1998, 5(3): 157–160. (in Chinese)Google Scholar
  6. [6]
    MO Hai-hong, ZENG He-pin, XIAO Gui-wu. Study on water-soluble products from Solanum nigrum L. [J]. Journal of South China Normal University, 1995, 18(2): 41–42. (in Chinese)Google Scholar
  7. [7]
    Kaufmann B, Christen P. Recent extraction techniques for natural products: microwave assisted extraction and pressurized solvent extraction [J]. Phytochemical Analysis, 2002, 13: 105–113.CrossRefGoogle Scholar
  8. [8]
    Ganzler K. A novel sample preparation method for chromatography[J]. Journal of Chromatography, 1986 (371): 299.Google Scholar
  9. [9]
    Brachet A, Christen P, Veuthey J. Focused Microwave-assisted extraction of cocaine and benzoylecgonine from coca leaves [J]. Phytochemical Analysis, 2002, 13: 162–169.CrossRefGoogle Scholar
  10. [10]
    Beatrice K, Philippe C. Parameters affecting microwave-assisted extraction of alkaloids[J]. Phytochemical Analysis, 2001, 12: 327–331.CrossRefGoogle Scholar
  11. [11]
    Marc V, Roser R. Determination of arsenic species in oyster tissue by microwave-assisted extraction and liquid chromatography-atomic fluorescence detection [J]. Applied Organometallic Chemistry, 2001, 15: 658–666.CrossRefGoogle Scholar
  12. [12]
    Laura C, Nadjezda H. Ultrasonication and microwave assisted extraction of degradation products from degradable polyolefin blends aged in soil[J]. Journal of Applied Polymer Science, 2001, 79: 1101–1112.CrossRefGoogle Scholar
  13. [13]
    Pare J R J, Signomin M, Lapointe J. Microwave-assisted natural products extraction[P]. US 5002784, 1991.Google Scholar
  14. [14]
    Pare J R J, Gloucesters J. Microwave extraction of volatile oil and apparatus[P]. Europe 0485668 A1, 1992.Google Scholar
  15. [15]
    Chen S S, Spiro M. Kinetics of microwave extraction of rosemary leaves in hexane, ethanol and a hexane ethanol mixture[J]. Flavour and Fragrance Journal, 1995(10): 101–122.CrossRefGoogle Scholar

Copyright information

© Central South University 2005

Authors and Affiliations

  • Chen Xiao-qing 
    • 1
    Email author
  • Liu qin 
    • 1
  • Jiang xin-yu 
    • 1
  • Zeng fan 
    • 1
  1. 1.School of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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