Adsorption characteristics of thiobacillus ferrooxidans on surface of sulfide minerals
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By using thiobacillus ferroxidans (T. f) from Qixiashan, Hubei Province, China, the adsorption characteristics of T. f on surface of sulfide mineral were studied. The influences of adsorption time, pH value, temperature, initial inoculated concentration of bacteria, concentration of sulfide mineral powder, and variety of minerals on the adsorption characteristics were firstly investigated by using the ninhydrin colorimetric method, and the changes of contact angles and Zeta potentials of mineral surface during the bacterial adsorption were then determined. The results show that when the leaching experiments are performed for a long time from several days to a month, the maximal quantity of adsorption of T f on the surface of pyrite is obtained under the following conditions: leaching for 20 d, pH value in range of 1–2 and temperature at 30 °C, respectively; when the bio-leaching experiments are performed for a shorter leaching time, the maximal quantity of adsorption is obtained under the conditions: bio-leaching for 2 h, at 2.4×107 cell/mL of initial inoculated bacteria concentration, and at 10% of mineral powder concentration; and the adsorption quantities are different from one sulfide mineral to another, and the adsorption of T. f on the surface of sulfide minerals includes three phases: increasing phase, stationary phase and decreasing phase.
Key wordsthiobacillus ferrooxidans sulphide mineral adsorption bio-leaching
CLC numberQ142 Q938.1+1
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- Malte H. The DLVO theory in microbial adhesion[J]. Colloids and Surfaces B: Biointerfaces, 1999, 14(1–4): 105–119.Google Scholar
- van Oss C J. Interfacial Forces in Aqueous Media[M]. New York: Marcel Dekker, 1994.Google Scholar
- TONG Xiong. The Theory and Practice of Microbe Leaching[M]. Beijing: Metallurgical Industry Press, 1997. (in Chinese)Google Scholar
- LI Xiu-yan, WEI De-zhou. Function of bacterial attachment on the pre-biooxidation of arsenic-bearing gold concentrate[J]. Journal of Northeastern University(Natural Science), 2000, 21(6): 641–644. (in Chinese)Google Scholar
- LI Yuan-gao, TANG Ai-dong. The Physiochemical Experiment Research Methods[M]. Changsha: Central South University Press, 2001. (in Chinese)Google Scholar
- LI Jian-wu, XIAO Neng-geng, YU Rui-yuan, et al. Physiochemical Experiment Principles and Methods [M]. Beijing: Peking University Press, 1994. (in Chinese)Google Scholar
- ZHOU Ji-kui, NIU Yin-jian. Protein content of mineral-adhered bacterium by ninhydrin colorimetric method[J]. Journal of Central South University of Technology(Natural Science), 2003, 34(2): 128–131. (in Chinese)Google Scholar