Journal of Central South University of Technology

, Volume 12, Issue 6, pp 671–676 | Cite as

Adsorption characteristics of thiobacillus ferrooxidans on surface of sulfide minerals

  • Liu Jian-she Email author
  • Xie Xue-hui 
  • Li Bang-mei 
  • Dong Qing-hai 


By using thiobacillus ferroxidans (T. f) from Qixiashan, Hubei Province, China, the adsorption characteristics of T. f on surface of sulfide mineral were studied. The influences of adsorption time, pH value, temperature, initial inoculated concentration of bacteria, concentration of sulfide mineral powder, and variety of minerals on the adsorption characteristics were firstly investigated by using the ninhydrin colorimetric method, and the changes of contact angles and Zeta potentials of mineral surface during the bacterial adsorption were then determined. The results show that when the leaching experiments are performed for a long time from several days to a month, the maximal quantity of adsorption of T f on the surface of pyrite is obtained under the following conditions: leaching for 20 d, pH value in range of 1–2 and temperature at 30 °C, respectively; when the bio-leaching experiments are performed for a shorter leaching time, the maximal quantity of adsorption is obtained under the conditions: bio-leaching for 2 h, at 2.4×107 cell/mL of initial inoculated bacteria concentration, and at 10% of mineral powder concentration; and the adsorption quantities are different from one sulfide mineral to another, and the adsorption of T. f on the surface of sulfide minerals includes three phases: increasing phase, stationary phase and decreasing phase.

Key words

thiobacillus ferrooxidans sulphide mineral adsorption bio-leaching 

CLC number

Q142 Q938.1+


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Copyright information

© Central South University 2005

Authors and Affiliations

  • Liu Jian-she 
    • 1
    Email author
  • Xie Xue-hui 
    • 1
  • Li Bang-mei 
    • 1
  • Dong Qing-hai 
    • 2
  1. 1.School of Resources Processing and BioengineeringCentral South UniversityChangshaChina
  2. 2.General Research Institute for Nonferrous MetalsBeijingChina

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