Advertisement

Kinetics of schiff base on Escherichia coli by microcalorimetry

  • Xu Ming-fei 
  • Li Xin-hai 
  • Wan Hong-wen 
  • Liu Yi 
Article
  • 45 Downloads

Abstract

The influence of four kinds of Schiff bases on a strain of Escherichia coli was studied by microcalorimetry. Differences in their capabilities of suppressing the metabolism of this bacterium were observed. The results show that the extent and duration of the inhibitory effect on the metabolism as judged from the multiplication rate constant, k, varies with different Schiff bases. The multiplication rate constant k, of Escherichia coli (in log phase) in the presence of Mo-salicylioaldehyde-thiadizole, Mo-piperonaldehyde-thiosemicarbazone and Mo-3-methoxy-salicylicaldehyde-thiadizole decreases with the increase of concentrations of compounds c, and the relationships between k and c, maximum heat production rate pm and c, peak time of growth curves tp and c are of linearity. For Mo-6-nitro-pieronalde-thiosemicarbazone, the multiplication rate constant is constant irrespective of variation in concentration. The sequence of antibiotic activity of Schiff base is: Mo-salicylioaldehyde-thiadizole>Mo-3-methoxy-salicylicaldehyde-thiadizole>Mo-piper-onaldehyde-thiosemicarbazone >6-nitro-pieronalde-thiosemicarbazone.

Key words

Escherichia coli kinetics microcalorimetry multiplication rate constant Schiff base inhibitory ratio metabolism 

Document code

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    ZHU Xin-de, WANG Cheng-gang, LE Zhi-feng. Synthesis and characterization and scavenger effect on superoxide of copper (II) and zinic (II) complexes derived from thiosemican bazide [J]. Synth React Inorg Met-Org Chem, 1991,21(9):1365–1373.CrossRefGoogle Scholar
  2. [2]
    Singh N K, Agrawal S, Aggarwal R C. Synthetic, structural and antifungal studies of some 3d-metal complexes of salicylaldhyde-2-furanthiocar boxyhydrazone [J]. Synth React Inorg Met-Org Chem, 1985,15(1):75–92.CrossRefGoogle Scholar
  3. [3]
    Soliman A A, Linert W. Investigation on new transition metal chelates of the 3-methoxy-salicylidene-2-aminothiopheaol Schiff base. Thermochim Acta, 1999,338:67–75.CrossRefGoogle Scholar
  4. [4]
    Wangner M R, Walker F A. Spectroscopic study of 1:1 copper (II) complexes with Schiff base ligands derived from salicylalblehyde and L-histidine and its analogy[J]. Inorg Chem, 1983,22:3021–3028.CrossRefGoogle Scholar
  5. [5]
    ZHU Xin-de, LE Zhi-feng, WANG Fu-yi, et al. Synthesis and biological activity of Schiff base from 4-chlorobenzaldehyde and glycine [J]. J Central China Normal University(Nat Sci) (in Chinese), 1995,29(2): 202–205.Google Scholar
  6. [6]
    LI Chong-jia, ZHANG Wen-tao, GAO Guo-lin, et al. A study on the Co(II) Schiff base complexes with carring O2 and N2 properties [J]. Acta Physico-Chimica (in Chinese), 1994,10(3):230–234.Google Scholar
  7. [7]
    LIU Yu-wen, WANG Cun-xing, ZHENG Cong-yi. Microcalorimetric study on the enhanced antitumor effects of 1-hexylcarbamoyl-5-fluorouracil by combination with hyperthermia on k-562 cell line. Thermochim Acta, 2001,369:51–57.CrossRefGoogle Scholar
  8. [8]
    Beezer A E, Fox G G, Gooch C A, et al. Microcalorimetric studies of the interaction of m-hydroxybenzoates with E. coli and with S. aureus. Demon stration of a colander relationship for biological response [J]. Int J Pharm, 1998,45:153–155.CrossRefGoogle Scholar
  9. [9]
    Hofner S, Svenson S, Beezer A E, Microcalorimetric studies of the interaction between antimycobacterial drugs and mycobacterium avium [J]. J Antimicrob Chemother, 1990,25:353–357.CrossRefGoogle Scholar
  10. [10]
    Alexandre G. S. Prado, Claudio Airodi. Effect of the pesticide 2,4-D on microbial activity of the soil monitored by microcalorimetry [J]. Thermochim Acta, 2000,(349):17–22.Google Scholar
  11. [11]
    LI Yu, HU Xing-gen, LIN Rui-sen. The effects of environmental conditions on the growth of petroleum microbes by microcalorimetry [J]. Thermochim Acta, 2000,(359):95–101.Google Scholar
  12. [12]
    ZHAO Dong-nan, YAN Xiang, ZHONG Yan-wang. Microcalorimetric investigation of growth conditions of petroleum bacteria [J]. Thermochim Acta, 1999, 338:1–6.CrossRefGoogle Scholar
  13. [13]
    XIE Chang-li, TANG Hou-kuan, SONG Zhao-hua, et al. Microcalorimetric study of bacterial growth [J]. Thermochim Acta, 1988,123:33–41.CrossRefGoogle Scholar
  14. [14]
    LIU Yi, FENG Ying, XIE Chang-li, et al. Microcalorimetric study on the effect of D-Glucasamine Schiff base and its complex on bacteria [J]. J Wuhan Univ (in Chinese), 1995,41(4):434–438.Google Scholar
  15. [15]
    FENG Ying, LIU Yi, XIE Chang-li, et al. Study on the comparative half inhibitory concentrations of Schiff base drugs effect on different bacteria [J]. Acta Physico-Chimica(in Chinese), 1996,12(3):229–234.Google Scholar

Copyright information

© Central South University 2003

Authors and Affiliations

  • Xu Ming-fei 
    • 1
  • Li Xin-hai 
    • 1
  • Wan Hong-wen 
    • 2
  • Liu Yi 
    • 3
  1. 1.School of Metallurgical Science and EngineeringCentral South UniversityChangshaChina
  2. 2.Department of ChemistryCentral China Normal UniversityWuhanChina
  3. 3.School of ChemistryWuhan UniversityWuhanChina

Personalised recommendations