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Journal of the American Oil Chemists' Society

, Volume 75, Issue 12, pp 1723–1727 | Cite as

Identification and estimation of tocotrienols in the annatto lipid fraction by gas chromatography-mass spectrometry

  • N. Frega
  • M. Mozzon
  • F. Bocci
Article

Abstract

The lipid fraction of annatto (Bixa orellana L.) seeds was extracted with a Soxhlet apparatus with n-hexane and isolated by thin-layer chromatography. The fatty-soluble antioxidant fraction contained only tocotrienols, mainly δ-tocotrienol, but no tocopherols. The presence of tocotrienols was confirmed by gas chromatography-mass spectrometry. The quantities of δ-tocotrienol were 140–147 mg/100 g dry seeds and 5.2–5.5% wt/wt of lipid extract, determined by gas chromatography and high-performance liquid chromatography, respectively. Currently no vegetable species seems to contain comparable concentrations of δ-tocotrienol.

Key words

Annatto chemical ionization mass spectrometry tocotrienols 

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Copyright information

© AOCS Press 1998

Authors and Affiliations

  1. 1.Dipartimento di Biotecnologie Agrarie ed AmbientalUniversità degli Studi di AnconaAnconaItaly
  2. 2.Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sez. Industrie et tecnologie AlimentariUniversità degli Studi di FirenzeFirenzeItaly

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