Journal of the American Oil Chemists' Society

, Volume 92, Issue 8, pp 1085–1092 | Cite as

Validation of a Method for Quantitation of Soybean Lectin in Commercial Varieties

  • Matthew L. Breeze
  • Elisa Leyva-Guerrero
  • Grant R. Yeaman
  • Yelena Dudin
  • Ryan Akel
  • Phil Brune
  • Fred Claussen
  • Cecil Dharmasri
  • Jenny Golbach
  • Rong Guo
  • Carl Maxwell
  • Laura Privalle
  • Hilary Rogers
  • Kai Liu
  • Guomin Shan
  • Michele Yarnall
  • Denise Thiede
  • Nancy Gillikin
Original Paper

Abstract

Soybean agglutinin (SBA) protein, also known as soybean lectin, is regarded as an anti-nutrient due to its negative effect on the ability of monogastric animals to gain weight following consumption of raw soybean seed. Historically, SBA has been measured using a time-consuming and cumbersome hemagglutination procedure. The objective of our research was to obtain a validated methodology that is precise and accurate in the measurement of SBA while allowing minimally equipped laboratories to effectively conduct the analysis, thus our focus was on evaluating an existing commercially available ELISA, an enzyme-linked-lectin-assay (ELLA), and a hybrid ELISA/ELLA. A new ELLA technique that can detect and quantify lectins was chosen and modified specifically for the quantitation of SBA in soybean seed. The proposed ELLA methodology is similar to a standard sandwich ELISA, and uses polyacrylamide-linked N-acetylgalactosamine (Gal–NAc–PAA) for a capture phase and the biotinylated version (Gal–NAc–PAA–Biotin) for detection. Based upon the validation data, the ELLA method can precisely and accurately determine soybean lectin levels in soybean seed. The validated ELLA method was used to quantify SBA in nine commercial soybean varieties introduced between 1972 and 2008 and demonstrated that the natural variability of SBA is subject to the effects of genotype and environment.

Keywords

Soybean Lectin Agglutinin Composition ELLA SBA Enzyme linked 

Supplementary material

11746_2015_2679_MOESM1_ESM.docx (35 kb)
Supplementary material 1 (DOCX 34 kb)

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Copyright information

© AOCS 2015

Authors and Affiliations

  • Matthew L. Breeze
    • 1
  • Elisa Leyva-Guerrero
    • 1
  • Grant R. Yeaman
    • 1
  • Yelena Dudin
    • 1
  • Ryan Akel
    • 2
  • Phil Brune
    • 3
  • Fred Claussen
    • 4
  • Cecil Dharmasri
    • 7
  • Jenny Golbach
    • 4
  • Rong Guo
    • 7
  • Carl Maxwell
    • 5
  • Laura Privalle
    • 7
  • Hilary Rogers
    • 8
  • Kai Liu
    • 8
  • Guomin Shan
    • 6
  • Michele Yarnall
    • 3
  • Denise Thiede
    • 9
  • Nancy Gillikin
    • 10
  1. 1.Regulatory Product Characterization and Safety CenterMonsanto CompanySt. LouisUSA
  2. 2.Nutritional Chemistry and Food SafetyCovanceMadisonUSA
  3. 3.Syngenta Biotechnology IncResearch Triangle ParkUSA
  4. 4.EPL BioAnalytical ServicesNianticUSA
  5. 5.DuPont PioneerDuPont Experimental StationWilmingtonUSA
  6. 6.Biotechnology Regulatory SciencesDow AgroSciencesIndianapolisUSA
  7. 7.Bayer CropScience LPMorrisvilleUSA
  8. 8.Eurofins Nutrition Analysis CenterDes MoinesUSA
  9. 9.BioDiagnostics Inc.River FallsUSA
  10. 10.BASF Corporation Agricultural Products GroupResearch Triangle ParkUSA

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