Journal of the American Oil Chemists' Society

, Volume 92, Issue 3, pp 423–430

FAME Production and Fatty Acid Profiles from Moist Chlorella sp. and Nannochloropsis oculata Biomass

  • Rui C. M. Alves Sobrinho
  • Laércio Vauchinski
  • Renata Rodrigues de Moura
  • Ednei G. Primel
  • Paulo C. V. Abreu
  • Marcelo G. Montes D’Oca
Original Paper


In the present study, we investigated the production of fatty acid methyl esters (FAME) from moist Chlorella sp. and Nannochloropsis oculata biomass using a hydrolysis–esterification process. Additionally, we evaluated for the first time the fatty acid profile before and after this process. Hydrolysis of the lipid fraction was performed on a moist biomass in the presence of differing amounts of an acid catalyst in both 50 and 100 % w/w water relative to the biomass. The esterification of the crude extracts of the free fatty acids (FFA) was then investigated. The experiments show that in the presence of 50 % w/w water relative to the biomass, the hydrolysis–esterification process results in higher FFA and FAME yields. The analysis of the fatty ester profiles did not reveal any degradation of the FFA from the microalgae biomass under the hydrolysis–esterification conditions. The results were compared with both extraction–transesterification and direct transesterification processes using dry biomass. The extraction–transesterification and hydrolysis–esterification processes resulted in similar FAME yields and similar profiles of the fatty esters from dry and moist biomass materials, respectively.


Fatty acid profile Microalgae Moist biomass Nannochloropsis oculata Chlorella sp. 


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Copyright information

© AOCS 2015

Authors and Affiliations

  • Rui C. M. Alves Sobrinho
    • 1
  • Laércio Vauchinski
    • 1
  • Renata Rodrigues de Moura
    • 1
  • Ednei G. Primel
    • 1
  • Paulo C. V. Abreu
    • 2
  • Marcelo G. Montes D’Oca
    • 1
  1. 1.Laboratório Kolbe de Síntese Orgânica, Escola de Química e AlimentosUniversidade Federal do Rio GrandeRio GrandeBrazil
  2. 2.Laboratório de Ecologia do Fitoplâncton e de Microorganismos Marinhos, Instituto de OceanografiaUniversidade Federal do Rio GrandeRio GrandeBrazil

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