Phytochemical Composition of Oryza sativa (Rice) Bran Oil Bodies in Crude and Purified Isolates
- 662 Downloads
We describe a procedure for isolating and purifying oil bodies (OBs) from Oryza sativa bran, and present evidence that strongly suggests a physical association between the OB organelles and several antioxidant phytochemicals (γ-oryzanol and several tocochromanols). This suggestion arises from work showing that despite comprehensive washing steps (9 M urea, water), the majority of the tocotrienols and tocopherols (77 and 73 %, respectively) and oryzanols (91 %) remain integral to the oil bodies, not being released unless the latter are sacrificed. This in turn provides a valuable comparison with similar analyses of tocochromanols in other plant species, as well as indicating that rice bran, normally a waste product from the rice industry, may provide a valuable source of antioxidants as well as protein and unsaturated fat.
KeywordsRice bran Oil bodies Oxidation Tocotrienols Oryzanols
The authors would like to thank the Agricultural Research Development Agency, Thailand for supporting this work and East End Food plc. (Birmingham, UK) for the supply of brown rice in this study.
- 1.McCaskill DR, Zhang F (1999) Use of rice bran oil in foods. Food Technol 53:50–52Google Scholar
- 14.Tzen JTC, Cao YZ, Laurent P, Ratnayake C, Huang AHC (1993) Lipids, proteins, and structure of seed oil bodies from diverse species. Plant Physiol 101:267–276Google Scholar
- 22.Stryer L (2000) Biochemistry, 4th edn. W. H. Freeman, New YorkGoogle Scholar
- 25.Lin LJ, Tai SS, Peng CC, Tzen JT (2002) Steroleosin, a sterol-binding dehydrogenase in seed oil bodies. Plant Physiol 128:1200–1211Google Scholar