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Determination of Unsaponifiable Constituents of Deodorizer Distillates by GC–MS

  • Saba Naz
  • S. T. H. SheraziEmail author
  • Farah N. Talpur
  • M. Younis Talpur
  • Huseyin Kara
Original Paper

Abstract

Deodorizer distillate is an important by-product obtained during deodorization in the edible oil industries. It is a complex mixture of many health beneficial constituents like phytosterols, tocopherols and squalene. In the present study a simple gas chromatographic method with mass spectrometry was used for the separation, detection and quantification of different components present in the deodorizer distillate in a very short analysis time of 18 min. A simple saponification procedure without derivatization was used for their analysis followed by GC–MS analysis. Phytosterols concentration (21.27–25.53%) was the most abundant in canola and palm distillate samples whereas, squalene and tocopherol were present in concentration ranges of 2.89–13.21% and 1.29–5.81%, respectively. The present study revealed that the unsaponifiable fraction of deodorizer distillate could be used in cosmetic preparations due to its appreciable amount of bioactive constituents.

Keywords

Deodorizer distillate Bioactive components GC–MS 

Notes

Acknowledgments

The authors would like to thank to the National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan for providing financial support. Also thanks are due to the management of Shujabad Agro Industries (Pvt.) Ltd., Karachi, Pakistan for providing the samples to carry out the present research work.

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Copyright information

© AOCS 2012

Authors and Affiliations

  • Saba Naz
    • 1
  • S. T. H. Sherazi
    • 1
    Email author
  • Farah N. Talpur
    • 1
  • M. Younis Talpur
    • 1
  • Huseyin Kara
    • 2
  1. 1.National Center of Excellence in Analytical ChemistryUniversity of SindhJamshoroPakistan
  2. 2.Fen-Edebiyat Fakültesi, Kimya BölümüSelcuk UniversityKonyaTurkey

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