Journal of the American Oil Chemists' Society

, Volume 86, Issue 4, pp 393–400 | Cite as

Effect of Aqueous Enzymatic Processes on Sunflower Oil Quality

  • Sajid Latif
  • Farooq AnwarEmail author
Original Paper


The use of enzymes in aqueous vegetable oilseed extraction for simultaneous recovery of high quality oil and protein is gaining recognition. In the present work, five enzyme preparations [Protex 7L by Genencor (Rochester, NY USA), Alcalase 2.4L, and Viscozyme L by Novozymes (Bagsvaerd, Denmark), Natuzyme by Bioproton Pty Ltd (Australia) and Kemzyme by Kemin Europa N·V. (Belgium)] were studied to evaluate their effects on the extraction of oil and protein from sunflower seeds. Preliminary experiments were conducted for the selection of enzymes, optimum enzyme concentration, incubation time and pH. Maximum oil yield (87.25% of the total oil in the seed) was obtained with Viscozyme L, whereas, Protex 7L offered the highest level of protein in the aqueous phase. The comparison of the quality attributes of enzyme-assisted aqueous extracted (EAAE) oil with those of solvent-extracted and control (oils extracted without enzyme treatment) oils revealed no significant (> 0.05) variations for iodine value, density, refractive index, unsaponifiable matter, and fatty acid composition among the extraction methods. The control and EAAE oils also exhibited a better oxidation state. The tocopherol concentration for the oils, produced with the enzymes, was noted to be quite improved relative to the control and solvent-extracted oils. A higher antioxidant activity in terms of total phenolic contents, 2,2′-diphenyl-1-picrylhydrazyl scavenging capacity and inhibition of linoleic acid peroxidation was also observed for the EAAE oils as against control and the solvent-extracted oils.


Sunflower oil Enzyme-assisted aqueous extraction Physico-chemical properties FAs Tocopherols Oxidative stability Comparison 



The authors are thankful to the Higher Education Commission (HEC), of Pakistan for providing funding under the scheme “HEC Indigenous Scholarship” to accomplish the present research work.


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Copyright information

© AOCS 2009

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryUniversity of AgricultureFaisalabadPakistan

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