Journal of the American Oil Chemists' Society

, Volume 83, Issue 11, pp 943–946 | Cite as

Chemical analysis of ozonized theobroma fat

  • Maritza F. Díaz GómezEmail author
  • Goitybell Martínez Téllez
  • Maikel Arteaga Cruz
  • Rafael Garcés Mancheno


Ozonized theobroma fat is used as raw material in the manufacture of pessaries and cosmetic creams. Ozonization of theobroma fat with water was carried out at different applied ozone dosages, and the resultant PV, acid value, iodine value, total hydroperoxide content, and FA content were determined. PV and total hydroperoxide content showed a notable increase with applied ozone dosage up to 35.7 mg/g. Acid value varied slightly from 4.1 to 9.9 mg KOH/g, and the iodine value fell to zero. PV and total hydroperoxide content increased slightly with a higher applied ozone dosage. The comparison of total hydroperoxide measurement using ferrous oxidation in xylenol orange assay and traditional iodometric assay for PV determination showed a significant linear correlation. Small amounts of oleic acid were found in ozonized theobroma fat samples with iodine value equaling zero, which demonstrated that iodine value determination is an inexact assay. During ozonization of theobroma fat, an increase in acid value of 18.9-fold with respect to the initial value was observed owing to decomposition of peroxide.

Key Words

Acid value fatty acids gas-liquid chromatography hydroperoxides iodine value oleic acid ozonized theobroma fat peroxide value 


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  1. 1.
    Gunstone, F.D., Major Vegetable Fats: Cocoa Butter, in The Lipid Handbook, 2nd ed., edited by F.D. Gunstone, J. Harwood, and F. Padley, Chapman & Hall, London, 1994, pp. 57–102.Google Scholar
  2. 2.
    Chaiseri, S., and P. Dimick, Lipid and Hardness Characteristics of Cocoa Butters from Different Geographic Regions, J. Am. Oil Chem. Soc. 66: 1771–1776 (1989).Google Scholar
  3. 3.
    Tagaki, T., and Y. Ando, Stereospecific Analysis of Monounsaturated Triacylglycerols in Cocoa Butter, —Ibid. 72:1203–1206 (1995).CrossRefGoogle Scholar
  4. 4.
    Vajda, O.I., and T.W. Saenz, Química de los Alimentos, Editorial Científico Técnico, La Habana, 1976, Tomo I, pp. 15–20.Google Scholar
  5. 5.
    Pryor, W.A., G.L. Squadrito, and M. Friedman, The Cascade Mechanism to Explain Ozone Toxicity: The Role of Lipid Ozonation Products, Free Radic. Biol. Med. 19: 935–941 (1995).CrossRefGoogle Scholar
  6. 6.
    Bailey, P., Ozonolysis of Olefins: Introduction, Initial Ozone Attack and Adduct: The Peroxides Products, in Ozonization in Organic Chemistry Academic Press, New York, 1978, Vol. 1, pp. 9–37.Google Scholar
  7. 7.
    Pryor, W., and M. Wu, Ozonation of Methyl Oleate in Hexane, in a Thin Film, in SDS Micelles, and in Distearoylphosphatidylcholine Liposomes: Yields and Properties of the Criegge Ozonide, Chem. Res. Toxicol. 5:505–511 (1992).CrossRefGoogle Scholar
  8. 8.
    Diaz, M., I. Lezcano, J. Molerio, and F. Hernández, Spectroscopy Characterization of Ozonides with Biological Activity, Ozone Sci. Eng. 23:35–40 (2001).Google Scholar
  9. 9.
    Lezcano, I., J. Molerio, M. Gómez, R. Contreras, G. Roura, and W. Díaz, Actividad in vitro del OLEOZON Frente a Agentes Etiológicos de Infecciones de la Piel, Rev. CENIC Cien, Biol. 29:209–212 (1998).Google Scholar
  10. 10.
    Lezcano, I., N. Nuñez, M. Gutierrez, J. Molerio, M.G. Regüeiferos, and W. Díaz, Actividad in vitro del Aceite de Girasol Ozonizado (Oleozón) Frente a Diferentes Especies Bacterianas, —Ibid. 27: 46–49 (1996).Google Scholar
  11. 11.
    Sechi, L., I. Lezcano, N. Nuñez, M. Espim, I. Dupre, and A. Pinna, Antibacterial Activity of Ozonized Sunflower Oil (OLEOZON), J. Appl. Microbiol. 90:279–284 (2001).CrossRefGoogle Scholar
  12. 12.
    Lezcano, I., G. García, G.Martínez, J. Molerio, Z. Zamora, C. Fernández, C. González, and D.Castañeda, Efectividad de la Manteca de Cacao Ozonizada para el Tratamiento de la Candidiasis Vaginal, Rev. CENIC Cien. Biol. 29:206–208 (1998).Google Scholar
  13. 13.
    Hernández, R., G. Martínez, and M. Díaz, Aspectos Químico-Físicos del Aceite de Girasol Ozonizado, Rev. CENIC Cien. Quím, 35:159–162 (2004).Google Scholar
  14. 14.
    Nourooz-Zadeh, J., J. Tajaddini-Sarmadi, I. Birlouez-Aragon, and S. Wolf, Measurement of Hydroperoxides in Edible Oils Using the Ferrous Oxidation in Xylenol Orange Assay. J. Agric. Food Chem. 43:17–21 (1995).CrossRefGoogle Scholar
  15. 15.
    Martínez, G., R. Hernández, and M. Díaz, Determinación de Hidroperóxidos en Aceite de Girasol Ozonizado, Rev. Grasas Aceites 56:148–153 (2005).Google Scholar
  16. 16.
    Kamm, W., F. Dionisi, L.B. Fay, C. Hischenhuber, H.G. Schmarr, and K.H. Engel, Analysis of Steryl Esters in Cocoa Butter by On-line Liquid Chromatography—Gas Chromatography, J. Chromatogr. A918:341–349 (2001).CrossRefGoogle Scholar
  17. 17.
    Ledea, O., M. Díaz, J. Molerio, D. Jardines, A. Rosado, and T. Correa, Comparative 1H-NMR Study of Oleic Acid and Methyl Oleate Ozonation in a Bubbling Reactor, Proceedings of XIII Ozone World Congress, Kyoto, Japan, 1997, p. B1-1-1.Google Scholar
  18. 18.
    Peroxide Value, British Pharmacopoeia, British Pharmacopoeia Commission, London, 2000, Appendix XF, IA, IB.Google Scholar
  19. 19.
    Acid Value, Ibid. ppendix XB, IA, IB.Google Scholar
  20. 20.
    Iodine Value, Ibid., Appendix XE, IA, IB.Google Scholar
  21. 21.
    Thielmann, K., Principios de Metodología en Bioquímica Clínica. Ed. Organismos, Ciudad de la Habana, 1985, pp. 47–49.Google Scholar
  22. 22.
    Garcés, R. and M. Mancha, One-Step Lipid Extraction and Fatty Acid Methyl Esters Preparation from Fresh Plant Tissues, Anal. Biochem. 211:139–143 (1993).CrossRefGoogle Scholar
  23. 23.
    Criegee, R., Course of Ozonation of Unsaturated Compounds. Rec. Chem. Prog. 18:111–120 (1957).Google Scholar
  24. 24.
    Leikauf, G.D., Q. Zhao., S. Zhou, and J. Santrock, Activation of Eicosanoid Metabolism in Human Airway Epithelial Cells by Ozonoloysis Products of Membrane Fatty Acids, Res. Rep. Health Eff. Inst. 71:1–17 (1995).Google Scholar
  25. 25.
    Mehlenbacher, V., Determinación de Impurezas, in Análisis de Grasas y Aceites. Enciclopedia de la Química Industrial, Ediciones Urmo, Bilbao 1970, Vol. 6, pp. 114–116.Google Scholar
  26. 26.
    Díaz, M., R. Hernández, G. Martínez, G. Vidal, M. Gómez, H. Fernández, and R. Garcés, Comparative Study of Ozonized Olive Oil and Ozonized Sunflower Oil, J. Braz. Chem. Soc. 17:403–407 (2006).CrossRefGoogle Scholar

Copyright information

© AOCS Press 2006

Authors and Affiliations

  • Maritza F. Díaz Gómez
    • 1
    Email author
  • Goitybell Martínez Téllez
    • 1
  • Maikel Arteaga Cruz
    • 1
  • Rafael Garcés Mancheno
    • 2
  1. 1.Department of Ozonized Substances, Ozone Research CenterNational Center for Scientific ResearchHavanaCuba
  2. 2.Instituto de la Grasa (Consejo Superior de Investigaciónes CientíficasSevillaEspaña

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