Journal of the American Oil Chemists' Society

, Volume 84, Issue 3, pp 205–209 | Cite as

The Effect of Operational Parameters of the Rancimat Method on the Determination of the Oxidative Stability Measures and Shelf-Life Prediction of Soybean Oil

Original Paper


Operational parameters of the Rancimat method, including oil sample size, airflow rate, and temperature, were evaluated to determine their effects on the oxidative stability index (OSI), temperature coefficient, Q10 number, and shelf-life prediction for soybean oil. Operational parameters of the Rancimat method had statistically significant effects (P < 0.05) on the OSI. Whenever the oil sample size and airflow rate at a given temperature were such that the air-saturated condition could be established, the OSIs showed no statistically significant differences. As temperature increased, OSIs decreased, while their average coefficient of variation (CV) increased. In general, the conditions where the sample was saturated with air and had a relatively lower CV were an oil sample size of 6 g at all temperatures and airflow rates, then 3-g oil sample size at low temperatures (100 and 110 °C) and low airflow rates (10 and 15 L h−1). The temperature coefficient and Q10 number were found to be independent of the oil sample size and airflow rate, and their mean values for soybean oil were calculated to be −3.12 × 10−2 °C−1 and 2.05, respectively. Oil sample size and airflow rate showed a significant effect on shelf-life prediction for soybean oil. Therefore, choosing the right levels of these operational parameters in the Rancimat method may produce the least possible difference between predictions from long-term storage studies and the OSI test.


Oxidative stability index Q10 number Rancimat Shelf-life prediction Soybean oil Temperature coefficient 



The author thanks the Institute of Standards and Industrial Research of Iran (Mashhad branch) for providing research facilities for this work. Furthermore, the author is grateful to S.M.R. Moosavi and A. Heidari for providing the data of the determinations from the Rancimat test.


  1. 1.
    Hadorn H, Zurcher K (1974) Zur Bestimmung der Oxydations-stabilitat von Ölen und Fetten. Dtsch Lebensm Rundsch 70:57–65Google Scholar
  2. 2.
    Mendez E, Sanhueza J, Speisky H, Valenzuela A (1996) Validation of the Rancimat test for the assessment of the relative stability of fish oils. J Am Oil Chem Soc 73:1033–1037CrossRefGoogle Scholar
  3. 3.
    Hasenhuettl GL, Wan PJ (1992) Temperature effects on the determination of oxidative stability with the Metrohm Rancimat. J Am Oil Chem Soc 69:525–527Google Scholar
  4. 4.
    Loury M (1972) Possible mechanisms of autoxidative rancidity. Lipids 7:671–675CrossRefGoogle Scholar
  5. 5.
    deMan JM, Tie F, deMan L (1987) Formation of short chain volatile organic acids in the automated AOM method. J Am Oil Chem Soc 64:993–996CrossRefGoogle Scholar
  6. 6.
    Matthaus BW (1996) Determination of the oxidative stability of vegetable oils by Rancimat and conductivity and chemiluminescence measurements. J Am Oil Chem Soc 73:1039–1043CrossRefGoogle Scholar
  7. 7.
    Knothe G, Dunn RO (2003) Dependence of oil stability index of fatty compounds on their structure and concentration and presence of metals. J Am Oil Chem Soc 80:1021–1026CrossRefGoogle Scholar
  8. 8.
    Laubli MW, Bruttel PA (1986) Determination of the oxidative stability of fats and oils: comparison between the active oxygen method (AOCS Cd. 12–57) and the Rancimat method. J Am Oil Chem Soc 63:772–795CrossRefGoogle Scholar
  9. 9.
    Gordon MH, Mursi E (1994) A comparison of oil stability based on the Metrohm Rancimat with storage at 20 °C. J Am Oil Chem Soc 71:649–651CrossRefGoogle Scholar
  10. 10.
    Coppin EA, Pike OA (2001) Oil stability index correlated with sensory determination of oxidative stability in light-exposed soybean oil. J Am Oil Chem Soc 78:13–18CrossRefGoogle Scholar
  11. 11.
    Anwar F, Bhanger MI, Kazi TG (2003) Relationship between Rancimat and active oxygen method values at varying temperatures for several oils and fats. J Am Oil Chem Soc 80:151–155CrossRefGoogle Scholar
  12. 12.
    Kowalski B, Ratusz K, Kowalska D, Bekas W (2004) Determination of the oxidative stability of vegetable oils by differential scanning calorimetry and Rancimat measurements. Eur J Lipid Sci Technol 106:165–169CrossRefGoogle Scholar
  13. 13.
    Velasco J, Andersen ML, Skibsted LH (2004) Evaluation of oxidative stability of vegetable oils by monitoring the tendency to radical formation. A comparison of electron spin resonance spectroscopy with the Rancimat method and differential scanning calorimetry. Food Chem 85:623–632CrossRefGoogle Scholar
  14. 14.
    Kaya A, Tekin AR, Oner MD (1993) Oxidative stability of sunflower oil and olive oils: comparison between a modified oxygen method and long term storage. Food Sci Technol 26:464–468Google Scholar
  15. 15.
    Toro-Vazquez JF, Castillo AA, Hernandez-C R (1993) A multiple-variable approach to study corn oil oxidation. J Am Oil Chem Soc 70:261–267CrossRefGoogle Scholar
  16. 16.
    Presa-Owens S, Lopez-Sabater MC, Rivero-Urgell M (1995) Shelf-life prediction of an infant formula using an accelerated stability test (Rancimat). J Agric Food Chem 43:2879–2882CrossRefGoogle Scholar
  17. 17.
    Frankel EN (1998) Lipid oxidation. The Only Press, Dundee, pp 99–114Google Scholar
  18. 18.
    Reynhout G (1991) The effect of temperature on the induction time of a stabilized oil. J Am Oil Chem Soc 68:983–984Google Scholar
  19. 19.
    Jebe TA, Matlock MG, Sleeter RT (1993) Collaborative study of the oil stability index analysis. J Am Oil Chem Soc 70:1055–1061Google Scholar
  20. 20.
    Hill SE, Perkins EG (1995) Determination of oxidation stability of soybean oil with the oxidative stability instrument: operation parameter effects. J Am Oil Chem Soc 72:741–743Google Scholar
  21. 21.
    Shantha NC, Decker EA (1994) Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids. J AOAC Int 77:421–424Google Scholar

Copyright information

© AOCS 2007

Authors and Affiliations

  1. 1.Food Science and Technology Department, Faculty of AgricultureFerdowsi University of MashhadMashhadIran

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