Journal of the American Oil Chemists' Society

, Volume 84, Issue 2, pp 181–187 | Cite as

Extraction of Lipids from Municipal Wastewater Plant Microorganisms for Production of Biodiesel

  • Stephen Dufreche
  • R. HernandezEmail author
  • T. French
  • D. Sparks
  • M. Zappi
  • E. Alley
Original Paper


Municipal wastewater treatment plants in the USA produce over 6.2 × 106 t of dried sewage sludge every year. This microorganism-rich sludge is often landfilled or used as fertilizer. Recent restrictions on the use of sewage sludge, however, have resulted in increased disposal problems. Extraction of lipids from sludge yields an untapped source of cheap feedstock for biodiesel production. Solvents used for extraction in this study include n-hexane, methanol, acetone, and supercritical CO2. The gravimetric yield of oil was low for nonpolar solvents, but use of polar solvents gave a considerably increased yield; however, the percentage of saponifiable material was less. Extraction of lipids with a mixture of n-hexane, methanol, and acetone gave the largest conversion to biodiesel compared with other solvent systems, 4.41% based on total dry weight of sludge. In situ transesterification of dried sludge resulted in a yield of 6.23%. If a 10% dry weight yield of fatty acid methyl esters is assumed, the amount of biodiesel available for production in the USA is 1.4 × 106 m3/year. Outfitting 50% of municipal wastewater plants for lipid extraction and transesterification could result in enough biodiesel production to replace 0.5% of the national petroleum diesel demand (0.7 × 106 m3).


Bacterial lipid extraction Biodiesel Biosolids In situ transesterification Municipal sewage sludge Solvent extraction 



This work was supported by research grants from the Department of Energy (DE-FG36-04GO14251) and DOE EPSCOR (DE-FG02-00ER45830). Jessy Avelar and Keith Barlow provided laboratory assistance.


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Copyright information

© AOCS 2006

Authors and Affiliations

  • Stephen Dufreche
    • 1
  • R. Hernandez
    • 1
    Email author
  • T. French
    • 1
  • D. Sparks
    • 1
  • M. Zappi
    • 2
  • E. Alley
    • 1
  1. 1.Renewable Fuels and Chemicals Laboratory, Chemical Engineering DepartmentMississippi State UniversityMississipi StateUSA
  2. 2.University of LouisianaLafayetteUSA

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