Journal of the American Oil Chemists' Society

, Volume 81, Issue 3, pp 281–284 | Cite as

Lipase-catalyzed esterification of glycerol and oleic acid

  • Yesim Yesiloglu
  • Ismail Kilic


The enzymatic synthesis of glycerides from glycerol and oleic acid in organic solvent was studied, and the optimal conditions for glyceride synthesis by lipases were established. Of the commercially available lipases that were investigated, Candida rugosa lipase and porcine pancreas lipase resulted in the highest extent of esterification. Iso-octane and hexane were particularly useful organic solvents in glyceride synthesis. The water content in the reaction mixture was of primary importance. For C. rugosa lipase and porcine pancreas lipase, the optimal water contents were 5 and 1%, respectively. Candida rugosa lipase and porcine pancreas lipase manifested contrasting positional specificities in glyceride synthesis.

Key Words

Esterification lipase organic solvent 


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Copyright information

© AOCS Press 2004

Authors and Affiliations

  1. 1.Department of ChemistryTrakya UniversityEdirneTurkey
  2. 2.Education of FacultyTrakya UniversityEdirneTurkey

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