Lipase-catalyzed esterification of glycerol and oleic acid
- 645 Downloads
The enzymatic synthesis of glycerides from glycerol and oleic acid in organic solvent was studied, and the optimal conditions for glyceride synthesis by lipases were established. Of the commercially available lipases that were investigated, Candida rugosa lipase and porcine pancreas lipase resulted in the highest extent of esterification. Iso-octane and hexane were particularly useful organic solvents in glyceride synthesis. The water content in the reaction mixture was of primary importance. For C. rugosa lipase and porcine pancreas lipase, the optimal water contents were 5 and 1%, respectively. Candida rugosa lipase and porcine pancreas lipase manifested contrasting positional specificities in glyceride synthesis.
Key WordsEsterification lipase organic solvent
Unable to display preview. Download preview PDF.
- 1.Tantrakulsiri, J., N. Jeyashoke, and K. Krisanangkura, Utilization of Rice Hull Ash as a Support Material for Immobilization of Candida cylindracea Lipase, J. Am. Oil Chem. Soc. 74:173–175 (1997).Google Scholar
- 3.Mukherjee, K.D., Lipase-Catalyzed Reactions for Modification of Fats and Other Lipids, Biocatalysis 3:277–293 (1990).Google Scholar
- 4.Ayorinde, F.O., C.P. Nwaonichao, V.N. Parchment, K.A. Bryant, M. Hassan, and M.T. Clayton, Enzymatic Synthesis and Spectroscopic Characterization of 1,3-Divernoloyl Glycerol from Vernonia galamensis Seed Oil, J. Am. Oil Chem. Soc. 70:129–132 (1993).Google Scholar
- 5.Ergan, F., M. Trani, and G. Andre, Use of Lipases in Multiphasic Systems Solely Composed of Substrates, 68:412–417 (1991).Google Scholar
- 6.Pecnik, S., and Z. Knez, Enzymatic Fatty Ester Synthesis, 69:261–265 (1992).Google Scholar
- 8.Lie, E., and G. Molin, Esterification of Polyunsaturated Fatty Acids with Lipases from Different Sources, Int. J. Food Sci. Technol. 27:73–76 (1992).Google Scholar
- 9.Huang, A.H.C., Plant Lipases, in Lipases: Their Structure, Biochemistry and Application, edited by B. Borgstrom and H.L. Brockman, Elsevier, Amsterdam, 1984, pp. 419–430.Google Scholar
- 11.Fomuso, L.B., and C.C. Akoh, Structured Lipids: Lipase-Catalyzed Interesterification of Tricaproin and Trilinolein, J. Am. Oil Chem. Soc. 75:405–410 (1998).Google Scholar