Advertisement

Polyunsaturated fatty acid concentrates from borage and linseed oil fatty acids

  • Tor-Chern Chen
  • Yi-Hsu Ju
Article

Abstract

A modified low-temperature solvent crystallization process was employed for the enrichment of polyunsaturated fatty acids (PUFA) in borage and linseed oil fatty acids. The effects of solvent, operation temperature, and solvent to free fatty acid (FFA) ratio on the concentration of PUFA were investigated. The best results were achieved when a mixture of 30% acetonitrile and 70% acetone was used as the solvent. With an operation temperature of −80°C and a solvent to FFA ratio of 30 mL/g, γ-linolenic acid (GLA) in FFA of saponified borage oil can be raised from 23.4 to 88.9% with a yield of 62.0%. At a yield of 24.9%, α-linolenic acid in linseed oil can be increased from 55.0 to 85.7%. The results of this work are comparable to the best results available in the literature.

Key Words

ALA borage oil GLA linseed oil low temperature solvent crystallization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sardessai, V.M., Nutritional Role of Polyunsaturated Fatty Acids, J. Nutr. Biochem. 3:154–166 (1992).CrossRefGoogle Scholar
  2. 2.
    British Nutrition Foundation, Nutritional Aspects of Fish. British Nutrition Foundation Briefing Paper no. 10, British Nutrition Foundation, London (1993).Google Scholar
  3. 3.
    International Society for the Study of Fatty Acids and Lipids, Recommendations for the Essential Fatty Acid Requirements for Infant Formula, International Society for the Study of Fatty Acids and Lipids News Letter 1:4–5 (1994).Google Scholar
  4. 4.
    Wanasundara, U.N., and F. Shahidi, Concentration of Omega-3 Polyunsaturated Fatty Acids of Seal Blubber Oil by Urea Complexation: Optimization of Condition, Food Chem. 65:41–49 (1999).CrossRefGoogle Scholar
  5. 5.
    Walker, T.H., H.D. Cochran, and G.J. Hulbert, Supercritical Carbon Dioxide Extraction of Lipids from Pythium irregulare, J. Am. Oil Chem. Soc. 76:595–602 (1999).Google Scholar
  6. 6.
    Yamamura, Y., and Y. Shimomura, Industrial High-Performance Liquid Chromatography Purification of Docosapentaenoic Acid Ethyl Ester from Single-Cell Oil, ——Ibid. 74:1435–1440 (1997).Google Scholar
  7. 7.
    Syed Rahmatullah, M.S.K., V.K.S. Shukla, and K.D. Mukherjee, γ-Linolenic Acid Concentrates from Borage Oil Fatty Acids via Lipase-Catalyzed Esterification, ——Ibid. 71:563–567 (1994).Google Scholar
  8. 8.
    Shimada, Y., A. Sugihara, M. Shibahiraki, H. Fujita, H. Nakano, T. Nagao, T. Terai, and Y. Tominaga, Purification of γ-Linolenic Acid from Borage Oil by a Two-Step Enzymatic Method, ——Ibid. 74:1465–1470 (1997).Google Scholar
  9. 9.
    Shimada, Y., K. Maruyama, A. Sugihara, S. Moriyama, and Y. Tominaga, Purification of Docosahexaenoic Acid from Tuna Oil by a Two-Step Enzymatic Method: Hydrolysis and Selective Esterification, ——Ibid. 74:1441–1446 (1997).Google Scholar
  10. 10.
    Gunstone, F.D., J. McLaughlan, C.M. Scrimgeour, and A.P. Watson, Improved Procedures for the Isolation of Pure Oleic, Linoleic, and Linolenic Acids or Their Methyl Esters from Natural Sources, J. Sci. Food Agric. 27:675–680 (1976).CrossRefGoogle Scholar
  11. 11.
    Krukonis, V.J., Supercritical Fluid Fractionation of Fish Oils: Concentration of Eicosapentaenoic Acid, J. Am. Oil Chem. Soc. 61:698–699 (1984).Google Scholar
  12. 12.
    Brown, J.B., Fractional Solvent Crystallization, ——Ibid. 32:646–652 (1955).Google Scholar
  13. 13.
    Schlenk, H., Crystallization of Fatty Acids, ——Ibid. 38:728–736 (1961).Google Scholar
  14. 14.
    Huang, F.C., Y.H. Ju, and C.W. Huang, Enrichment of γ-Linolenic Acid from Borage Oil via Lipase-Catalyzed Reactions, ——Ibid. 74:977–981 (1997).Google Scholar
  15. 15.
    Uksila, E., and I. Lehtinen, Crystallization of Linseed Oil Fatty Acids from Acetonitrile, Acta Chem. Scand. 20:1645–1650 (1966).CrossRefGoogle Scholar

Copyright information

© AOCS Press 2001

Authors and Affiliations

  1. 1.Department of Chemical EngineeringNational Taiwan University of Science and TechnologyTaipeiTaiwan

Personalised recommendations