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Antioxidant activity of crude tannins of canola and rapeseed hulls

  • R. Amarowicz
  • M. NaczkEmail author
  • F. Shahidi
Article

Abstract

The antioxidant activity of crude tannins of canola and rapeseed hulls was evaluated by β-carotene-linoleate, α,α-diphenyl-β-picrylhydrazyl (DPPH) radical, and reducing power assays. Crude tannins were extracted from three samples of Cyclone canola (high-tannin) hulls and Kolner, Ligaret, and Leo Polish rapeseed (low-tannin) hulls with 70% (vol/vol) acetone. The total phenolic content in crude tannin extracts ranged between 128 and 296 mg of sinapic acid equivalents per 1 g of extract. The ultraviolet spectra of methanolic solution of canola extracts showed two absorption maxima (282 and 309 nm), whereas those of rapeseed extracts exhibited a single maximum (326 nm). Crude tannins isolated from canola hulls exerted significantly (P<0.025) greater antioxidant activity than those from rapeseed in all three assays. The scavenging effect of all crude tannins, at a dose of 1 mg, on the DPPH radical ranged from 35.2 to 50.5%. The reducing power of Cyclone canola hull extracts on potassium ferricyanide was significantly (P≤0.0025) greater than that of rapeseed hull extracts, and the observed data correlated well (r=0.966; P=0.002) with the total content of phenolics present.

Key Words

Antioxidative properties canola condensed tannins hulls rapeseed reducing power scavenging effects 

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Copyright information

© AOCS Press 2000

Authors and Affiliations

  1. 1.Department of BiochemistryMemorial University of NewfoundlandSt. John'sCanada
  2. 2.Department of Human NutritionSt. Francis Xavier UniversityAntigonishCanada

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