Lipids

, Volume 34, Issue 4, pp 381–386

Metabolism of trans fatty acids by hepatocytes

  • Manuel Guzmán
  • Wil Klein
  • Teresa Gómez del Pulgar
  • Math J. H. Geelen
Article

DOI: 10.1007/s11745-999-0376-6

Cite this article as:
Guzmán, M., Klein, W., Gómez del Pulgar, T. et al. Lipids (1999) 34: 381. doi:10.1007/s11745-999-0376-6

Abstract

The present work was undertaken to study the metabolism of fatty acids with trans double bonds by rat hepatocytes. In liver mitochondria, elaidoyl-CoA was a poorer substrate for carnitine palmitoyltransferase I (CPT-I) than oleoyl-CoA. Likewise, incubation, of hepatocytes with oleic acid produced a more pronounced stimulation of CPT-I than incubation with trans fatty acids. This was not due to a differential effect of cis and trans fatty acids on acetyl-CoA carboxylase (ACC) activity and malonyl-CoA levels. Elaidic acid was metabolized by hepatocytes at a higher rate than oleic acid. Surprisingly, compared to oleic acid, elaidic acid was a better substrate for mitochondrial and, especially, peroxisomal oxidation, but a poorer substrate for cellular and very low density lipoprotein triacylglycerol synthesis. Results thus show that trans fatty acids are preferentially oxidized by hepatic peroxisomes, and that the ACC/malonyl-CoA/CPT-I system for coordinate control of fatty acid metabolism is not responsible for the distinct hepatic utilization of cis and trans fatty acids.

Abbreviations

ACC

acetyl-CoA carboxylase

CPT-I

carnitine palmitoyl-transferase I

TDGA

tetradecylglycidic acid

VLDL

very low density lipoprotein

Copyright information

© AOCS Press 1999

Authors and Affiliations

  • Manuel Guzmán
    • 1
  • Wil Klein
    • 2
  • Teresa Gómez del Pulgar
    • 1
  • Math J. H. Geelen
    • 2
  1. 1.Department of Biochemistry and Molecular Biology I, School of BiologyComplutense UniversityMadridSpain
  2. 2.Laboratory of Veterinary BiochemistryUltrecht UniversityTD UtrechtThe Netherlands

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