, Volume 34, Issue 3, pp 291–297 | Cite as

Fatty acid composition of 19 species of fish from the black sea and the Marmara Sea

  • Refik Tanakol
  • Zeliha Yazici
  • Erdal Şener
  • Ergin Sencer


Evidence suggests that differences in fatty acid composition among various fish species may be due to differences in diet or to environmental factors such as temperature, salinity, and depth at which the fish are caught. The beneficial effects of a diet containing fish on cardiovascular or other diseases have been associated with their high content of eicosapentaenoic (20∶5n-3) and docosahexaenoic (22∶6n-3) acids. In this study we analyzed the fatty acid composition of the flesh of 18 different species of marine fish and of cultured rainbow trout. The fish were obtained from the Black and the Marmara Seas, both of which have unique biological and ecological systems as well as eutrophication and pollution. The contents of 20∶5n-3 and 22∶6n-3 in the marine fish ranged from 4.2 to 13.3 wt% of total fatty acids, and from 6.6 to 40.8 wt%, respectively. The most important differences from other studies on oceanic fish were the tendencies toward higher percentages of 16∶0 and 22∶6n-3. The n-3 series of polyunsaturated fatty acids were present as 32.4±1.9% of the total fatty acids. The present study suggests that mature and immature Pomatomus saltator, as well as Engraulis encrasicolus, Mullus surmuletus, Sardina pilchardus, Mugil cephalus, and Sarda sarda may be preferred for the Turkish diet as a result of their high 20∶5 n-3 and 22∶6 n-3 contents. The cultured rainbow trout Oncorhynchus mykiss is not as good a source of n-3 fatty acids as are the marine fish.



docosahexaenoic acid


eicosapentaenoic acid


fatty acid


polyunsaturated fatty acids


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  1. 1.
    Bonaa, K.H., Bjerve, K.S., Straume, B., Gram, I.T., and Thelle, D. (1990) Effect of Eicosapentaenoic and Docosahexaenoic Acids on Blood Pressure in Hypertension, N. Engl. J. Med. 322, 795–801.PubMedCrossRefGoogle Scholar
  2. 2.
    Tidwell, D.K., McNaughton, J.P., Pellum, L.K., McLaurin, B.P., and Chen, S.C. (1993) Comparison of the Effects of Adding Fish High or Low in n-3 Fatty Acids to a Diet Conforming to the Dietary Guidelines for Americans, J. Am. Diet. Assoc. 93, 1124–1128.PubMedCrossRefGoogle Scholar
  3. 3.
    Siess, W., Roth, P., Scherer, B., Kurzmann, I., Böhlig, B., and Weber, P.C. (1980) Platelet-Membrane Fatty Acids, Platelet Aggregation, and Thromboxane Formation During a Mackerel Diet, Lancet 1, 441–444.PubMedCrossRefGoogle Scholar
  4. 4.
    Simopoulos, A.P. (1989) Summary of the NATO Advanced Research Workshop on Dietary Omega 3 and Omega 6 Fatty Acids: Biological Effects and Nutritional Essentiality, J. Nutr. 119, 521–528.PubMedGoogle Scholar
  5. 5.
    Clark, W.F., Parbtani, A., Huff, M.W., Reid, B., Holub, B.J., and Falardeau, P. (1989) Omega-3 Fatty Acid Dietary Supplementation in Systemic Lupus Erythematosus, Kidney Int. 36, 653–660.PubMedGoogle Scholar
  6. 6.
    Donadio, J.V. (1991) Omega-3 Polyunsaturated Fatty Acids: A Potential New Treatment of Immune Renal Disease, Mayo Clin. Proc. 66, 1018–1028.PubMedGoogle Scholar
  7. 7.
    Geusens, P., Wouters, C., Nijs, J., Jiang, Y., and Dequeker, J. (1994) Long-Term Effects of Omega-3 Fatty Acid Supplementation in Active Rheumatoid Arthritis. A 12-Month, Double-Blind, Controlled Study, Arthritis Rheum. 37, 824–829.PubMedGoogle Scholar
  8. 8.
    Karmali, R.A., Adams, L., and Trout, J.R. (1993) Plant and Marine n-3 Fatty Acids Inhibit Experimental Metastasis of Rat Mammary Adenocarcinoma Cells, Prostaglandins Leukotrienes Essent. Fatty Acids 48, 309–314.CrossRefGoogle Scholar
  9. 9.
    Noguchi, M., Rose, D.P., Earashi, M., and Miyazaki, I. (1995) The Role of Fatty Acids and Eicosanoid Synthesis Inhibitors in Breast Carcinoma, Oncology 52, 265–271.PubMedCrossRefGoogle Scholar
  10. 10.
    Belluzzi, A., Brignola, C., Campieri, M., Pera, A., Boschi, S., and Miglioli, M. (1996) Effect of an Enteric-Coated Fish-Oil Preparation on Relapses in Crohn's Disease, N. Engl. J. Med. 334, 1557–1560.PubMedCrossRefGoogle Scholar
  11. 11.
    Siscovick, D.S., Raghunathan, T.E., King, I., Weinmann, S., Wicklund, K.G., Albright, J., Bovjerg, V., Arbogast, P., Smith, H., Kushi, L.H., Cobb, L.A., Copass, M.K., Psaty, B.M., Lemaitre, R., Retzlaff, B., Childs, M., and Knopp, R.H. (1995) Dietary Intake and Cell Membrane Levels of Long Chain n-3 Polyunsaturated Fatty Acids and the Risk of Primary Cardiac Arrest, JAMA 274, 1363–1367.PubMedCrossRefGoogle Scholar
  12. 12.
    Bang, H.O., Dyerberg, J., and Sinclair, H.M. (1980) The Composition of the Eskimo Food in Northwestern Greenland, Am. J. Clin. Nutr. 33, 2657–2661.PubMedGoogle Scholar
  13. 13.
    Kromhout, D., Feskens, E.J., and Bowles, C.H. (1995) The Protective Effect of a Small Amount of Fish on Coronary Heart Disease Mortality in an Elderly Population, Int. J. Epidemiol. 24, 340–345.PubMedGoogle Scholar
  14. 14.
    Kromhout, D., Bosschieter, E.B., and Coulander, C. (1985) The Inverse Relation Between Fish Consumption and 20-Year Mortality from Coronary Heart Disease, N. Engl. J. Med. 312, 1205–1209.PubMedCrossRefGoogle Scholar
  15. 15.
    Kromann, N., and Green, A. (1980) Epidemiological Studies in the Upernavik District, Greenland: Incidence of Some Chronic Diseases 1950–1974, Acta Med. Scand. 208, 401–406.PubMedCrossRefGoogle Scholar
  16. 16.
    Yazici, Z., Tavares, I.A., Stamford, I.F., Bishai, P.M., and Bennett, A. (1992) Changes in Tissue Fatty Acid Composition in Murine Malignancy and Following Anticancer Therapy, Br. J. Cancer 65, 163–170.PubMedGoogle Scholar
  17. 17.
    Sigurgisladottir, S., and Palmadottir, H. (1993) Fatty Acid Composition of Thirty-Five Icelandic Fish Species, J. Am. Oil Chem. Soc. 70, 1081–1087.Google Scholar
  18. 18.
    Ackman, R.G. (1967) Characteristics of the Fatty Acid Composition and Biochemistry of Some Freshwater Fish Oils and Lipids in Comparison with Marine Oils and Lipids, Comp. Biochem. Physiol. 22, 907–922.CrossRefGoogle Scholar
  19. 19.
    Van Vliet, T., and Katan, M.B. (1990) Lower Ratio of n-3 to n-6 Fatty Acids in Cultured Than in Wild Fish, J. Clin. Nutr. 51, 1–2.Google Scholar
  20. 20.
    Castell, J.D. (1979) Review of Lipid Requirements of Finfish, in Finfish Nutrition and Fishfeed Technology (Halver, J.E., and Trews, K., eds.) Proceedings of a World Symposium, Sponsored and Supported by EIFAC/FAO/ICES/IUNS, 1978 June 20–23, Hamburg, Germany, Schriften der Bundesforschungsanstalt für Fischerci Hamburg (14/15) Vol. 1, pp. 59–84.Google Scholar
  21. 21.
    Belling, G.B., Abbey, M., Campbell, J.H., and Campbell, G.R. (1997) Lipid Content and Fatty Acid Composition of 11 Species of Queensland (Australia) Fish, Lipids 32, 621–625.PubMedGoogle Scholar
  22. 22.
    Puustinen, T., Punnonen, K., and Uotila, P. (1985) The Fatty Acid Composition of 12 North-European Fish Species, Acta Med. Scand. 218, 59–62.PubMedCrossRefGoogle Scholar
  23. 23.
    Halver, J.E. (1980) Lipids and Fatty Acids, in United Nations Development Programme. FAO of USA, Rome, ADCP/REP/80/11. Fish Feed Technology, Chapter 4, Rome, pp. 41–53.Google Scholar
  24. 24.
    Voss, A., Reinhart, M., Sankarappa, S., and Sprecher, H. (1991) The Metabolism of 7,10,13,16,19-Docosapentaenoic Acid to 4,7,10,13,16,19-Docosahexaenoic Acid in Rat Liver Is Independent of a 4-Desaturase, J. Biol. Chem. 266, 19995–20000.PubMedGoogle Scholar
  25. 25.
    Aveldaño, M.I., Robinson, B.S., Johnson, D.W., and Poulos, A. (1993) Long and Very Long Chain Polyunsaturated Fatty Acids of the n-6 Series in Rat Seminiferous Tubules, J. Biol. Chem. 268, 11663–11669.PubMedGoogle Scholar
  26. 26.
    Ackman, R.G. (1994) Seafood Lipids, in Seafoods: Chemistry, Processing Technology and Quality (Shahidi, F., and Botta, J.R., eds.) pp. 38–48, Blackie Academic & Professional, London.Google Scholar
  27. 27.
    Zaitsev, Y.P. (1991) Cultural Eutrophication of the Black Sea and Other South European Seas, La Mer 29, 1–7.Google Scholar
  28. 28.
    Zaitsev, Y.P. (1992) Recent Changes in the Trophic Structure of the Black Sea, Fisheries Oceanogr. 1,2, 180–189.Google Scholar
  29. 29.
    Nesterova, D.A. (1987) Peculiarities of Phytoplankton Successions in the Northwestern Part of the Black Sea, Hydrobiol. J. 23, 16–21.Google Scholar
  30. 30.
    Zaitsev, Y.P. (1993) Impact of Eutrophication on the Black Sea Fauna, in Studies and Reviews, Fisheries and Environment Studies in the Black Sea System, General Fisheries Council for the Mediterranean, Food and Agriculture Organization of the United Nations (FAO), Rome, 64, 59–86.Google Scholar
  31. 31.
    Sargent, J.R. (1997) Fish Oils and Human Diet, Br. J. Nutr. 78, S5-S13.PubMedCrossRefGoogle Scholar
  32. 32.
    Kocatas, A., Koray, T., Kaya, M., and Kara, O.F. (1993) Review of the Fishery Resources and Their Environment in the Marmara Sea, in Studies and Reviews Fisheries and Environment Studies in the Black Sea System, General Fisheries Council for the Mediterranean, Food and Agriculture Organization of the United Nations (FAO), Rome, 64, 87–143.Google Scholar
  33. 33.
    Bingel, F., Unsal, M., and Alavi, N. (1986) Biology of the Bosphorus and Its Entrances. Oceanography of the Turkish Straits, Middle East Technical University, Erdemli, Icel, Inst. Mar. Sci. 4, 69.Google Scholar
  34. 34.
    Ünlüata, U., and özsoy, E. (1986) Health of the Turkish Straits I: Oxygen Deficiency of the Marmara Sea. Oceanography of the Turkish Straits (First Annual Report), Middle East Technicaal University, Erdemli, Içel, Inst. Mar. Sci. 2, 78.Google Scholar
  35. 35.
    Daviglus, M.L., Stamler, J., Orencia, A.J., Dyer, A.R., Liu, K., Greenland, P., Walsh, M.K., Morris, D., and Shekelle, R.B. (1997) Fish Consumption and the 30-Year Risk of Fatal Myocardial Infarction, N. Engl. J. Med. 336, 1046–1053.PubMedCrossRefGoogle Scholar
  36. 36.
    Burr, M.L. (1989) Fish and the Cardiovascular System, Prog. Food Nutr. Sci. 13, 291–316.PubMedGoogle Scholar
  37. 37.
    Clandinin, M.T., Jumpsen, J., and Miyoung, S. (1994) Relationship Between Fatty Acid Accretion, Membrane Composition, and Biological Functions, J. Pediatr. 125, S25-S32.PubMedGoogle Scholar
  38. 38.
    Neuringer, M., Reisbick, S., and Janowsky, J. (1994) The Role of n-3 Fatty Acids in Visual and Cognitive Development: Current Evidence and Methods of Assessment, J. Pediatr. 125, S39-S47.PubMedGoogle Scholar

Copyright information

© AOCS Press 1999

Authors and Affiliations

  • Refik Tanakol
    • 3
  • Zeliha Yazici
    • 1
  • Erdal Şener
    • 2
  • Ergin Sencer
    • 3
  1. 1.Department of Pharmacology, Cerrahpaşa Faculty of MedicineUniversity of IstanbulTurkey
  2. 2.Faculty of Aquatic Products, Department of AquacultureUniversity of IstanbulTurkey
  3. 3.Department of Endocrinology, Metabolism and Nutrition, Istanbul Faculty of MedicineUniversity of IstanbulIstanbulTurkey

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