Lipids

, Volume 33, Issue 1, pp 11–21

Effect of modified dairy fat on postprandial and fasting plasma lipids and lipoproteins in healthy young men

  • Tine Tholstrup
  • Brittmarie Sandström
  • John E. Hermansen
  • Gunhild Hølmer
Article

DOI: 10.1007/s11745-998-0175-0

Cite this article as:
Tholstrup, T., Sandström, B., Hermansen, J.E. et al. Lipids (1998) 33: 11. doi:10.1007/s11745-998-0175-0

Abstract

Fatty acid profile of milk fat can be modified by cow feeding strategies. Our aim was postprandially and after 4 wk to compare the effect of a modified milk fat (M diet) [with 16% of the cholesterolemic saturated fatty acid (C12–16) replaced by mainly oleic and stearic acids] with the effect of D diet, including a conventional Danish milk fat on plasma lipids and lipoproteins. A side effect of the cow feeding regime was a 5% (w/w) increase in trans fatty acid in M diet. Eighteen subjects were fed for two periods of 4 wk strictly controlled isoenergetic test diets with 40% of energy from total fat and the same content of dietary cholesterol in a randomized study with cross-over design. Contrary to expectations, fasting low density lipoprotein (LDL) cholesterol concentration did not differ after the experimental periods. However, M diet resulted in a higher fasting total triacylglycerol concentration compared to D diet (P=0.009). Postprandial samples were taken at two different occasions (i) at day 21, after breakfast and lunch and (ii) on the last day of the study 2, 4, 6, and 8 h after a fat load. Postprandial plasma triacylglycerol and chylomicron triacylglycerol showed higher peak values after D diet than M diet (interaction effect, diet × times P<0.05). In conclusion, M diet did not lower LDL cholesterol compared to D diet. Thus any cholesterol-lowering effect of oleic and stearic acids may have been obscured by the high content of cholesterol-raising saturated fatty acids in milk fat. A higher content of the trans fatty acids in M diet might have counteracted the cholesterol neutral/decreasing effect and increased plasma triacylglycerol.

Abbreviations

apo

apoprotein

CE

cholesterol esters

CLA

conjugated linoleic acid

D

diet, Danish milk fat

20:3n−6

dihomo-γ-linolenic acid

22:6n−3

docosahexaenoic acid

22:5n−3

docosapentaenoic acid

E%

energy percentage

HDL

high density lipoprotein

12:0

lauric acid

LDL

low density lipoprotein

M

diet, modified milk fat

MUFA

monounsaturated fatty acids

14:0

myristic acid

16:0

palmitic acid

PL

phospholipid

PUFA

polyunsaturated fatty acids

SFA

saturated fatty acid

18:0

stearic acid

TAG

triacylglycerol

18:1n−7

vaccenic acid

VLDL

very low density lipoprotein

Copyright information

© AOCS Press 1998

Authors and Affiliations

  • Tine Tholstrup
    • 1
    • 4
  • Brittmarie Sandström
    • 1
    • 4
  • John E. Hermansen
    • 2
  • Gunhild Hølmer
    • 3
  1. 1.Centre for Advanced Food StudiesThe Royal Veterinary and Agricultural UniversityFrederiksbergDenmark
  2. 2.Department of Product QualityDanish Institute of Animal ScienceFoulumDenmark
  3. 3.Department of Biochemistry and NutritionTechnical University of DenmarkLyngby
  4. 4.Research Department of Human NutritionThe Royal Veterinary and Agricultural UniversityFrederiksbergDenmark

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