Effect of conjugated linoleic acid on body composition in mice
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The effects of conjugated linoleic acid (CLA) on body composition were investigated. ICR mice were fed a control diet containing 5.5% corn oil or a CLA-supplemented diet (5.0% corn oil plus 0.5% CLA). Mice fed CLA-supplemented diet exhibited 57% and 60% lower body fat and 5% and 14% increased lean body mass relative to controls (P<0.05). Total carnitine palmitoyltransferase activity was increased by dietary CLA supplementation in both fat pad and skeletal muscle; the differences were significant for fat pad of fed mice and skeletal muscle of fasted mice. In cultured 3T3-L1 adipocytes CLA treatment (1×10−4 M) significantly reduced heparin-releasable lipoprotein lipase activity (−66%) and the intracellular concentrations of triacylglyceride (−8%) and glycerol (−15%), but significantly increased free glycerol in the culture medium (+22%) compared to control (P<0.05). The effects of CLA on body composition appear to be due in part to reduced fat deposition and increased lipolysis in adipocytes, possibly coupled with enhanced fatty acid oxidation in both muscle cells and adipocytes.
KeywordsLinoleic Acid Body Composition Conjugate Linoleic Acid Feed Intake Free Glycerol
conjugated linoleic acid
Dulbecco’s modified Eagle’s medium
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- 7.Nicolosi, R.J., Courtemanche, K.V., Laitinen, L., Scimeca, J.A., and Huth, P.J. (1993) Effect of Feeding Diets Enriched in Conjugated Linoleic Acid on Lipoproteins and Aortic Atherogenesis in Hamsters, Circulation 88 (suppl.), 2458.Google Scholar
- 8.Cook, M.E., Miller, C.C., Park, Y., and Pariza, M.W. (1993) Immune Modulation by Altered Nutrient Metabolism: Nutritional Control of Immune-Induced Growth Depression, Poultry Sci. 72, 1301–1305.Google Scholar
- 11.Helrich, K. (1990) Official Methods of Analysis, 15th edn., pp. 935–937, Association of Official Analytical Chemists Inc., Arlington.Google Scholar
- 15.Lynen, F. (1962) Fatty Acid Synthesis from Malonyl CoA, Methods Enzymol. 1, 739–741.Google Scholar
- 19.Bieber, L.L., Abraham, T., and Helmrath, T. (1972) A Rapid Spectrophotometric Assay for Carnitine Palmitoyltransferase, Anal. Chem. 50, 509–518.Google Scholar
- 22.Pariza, M.W., Park, Y., Kim, S., Sugimoto, K., Albright, K., Liu, W., Storkson, J., and Cook, M. (1997) Mechanism of Body Fat Reduction by Conjugated Linoleic Acid, FASEB J. 11, A139.Google Scholar
- 33.Endres, S., Ghorbani, R., Kelley, V.E., Georgilis, K., Lonnemann, G., van der Meer, J.W.M., Cannon, J.G., Rogers, T.S., Klempner, M.S., Weber, P.C., Schaefer, E.J., Wolff, S.M., and Dinarello, C.A. (1989) The Effect of Dietary Supplementation with n−3 Polyunsaturated Fatty Acids on the Synthesis of Interleukin-1 and Tumor Necrosis Factor by Mononucler Cells, New Engl. J. Med. 320, 265–271.PubMedCrossRefGoogle Scholar