Lipids

, Volume 32, Issue 8, pp 853–858 | Cite as

Effect of conjugated linoleic acid on body composition in mice

  • Yeonhwa Park
  • Karen J. Albright
  • Wei Liu
  • Jayne M. Storkson
  • Mark E. Cook
  • Michael W. Pariza
Article

Abstract

The effects of conjugated linoleic acid (CLA) on body composition were investigated. ICR mice were fed a control diet containing 5.5% corn oil or a CLA-supplemented diet (5.0% corn oil plus 0.5% CLA). Mice fed CLA-supplemented diet exhibited 57% and 60% lower body fat and 5% and 14% increased lean body mass relative to controls (P<0.05). Total carnitine palmitoyltransferase activity was increased by dietary CLA supplementation in both fat pad and skeletal muscle; the differences were significant for fat pad of fed mice and skeletal muscle of fasted mice. In cultured 3T3-L1 adipocytes CLA treatment (1×10−4 M) significantly reduced heparin-releasable lipoprotein lipase activity (−66%) and the intracellular concentrations of triacylglyceride (−8%) and glycerol (−15%), but significantly increased free glycerol in the culture medium (+22%) compared to control (P<0.05). The effects of CLA on body composition appear to be due in part to reduced fat deposition and increased lipolysis in adipocytes, possibly coupled with enhanced fatty acid oxidation in both muscle cells and adipocytes.

Abbreviations

CLA

conjugated linoleic acid

CPT

carnitine palmitoyltransferase

DMEM

Dulbecco’s modified Eagle’s medium

LPL

lipoprotein lipase

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Copyright information

© AOCS Press 1997

Authors and Affiliations

  • Yeonhwa Park
    • 1
    • 3
  • Karen J. Albright
    • 1
    • 3
  • Wei Liu
    • 1
    • 3
  • Jayne M. Storkson
    • 1
    • 3
  • Mark E. Cook
    • 1
    • 2
    • 3
  • Michael W. Pariza
    • 1
    • 3
  1. 1.Department of Food Microbiology and ToxicologyUniversity of Wisconsin-MadisonMadison
  2. 2.Department of Animal SciencesUniversity of Wisconsin-MadisonMadison
  3. 3.Food Research InstituteMadison

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