Lipids

, Volume 50, Issue 10, pp 1029–1041 | Cite as

Modulation of the Expression of Components of the Stress Response by Dietary Arachidonic Acid in European Sea Bass (Dicentrarchus labrax) Larvae

  • Daniel Montero
  • Genciana Terova
  • Simona Rimoldi
  • Mónica B. Betancor
  • Eyad Atalah
  • Silvia Torrecillas
  • María J. Caballero
  • María J. Zamorano
  • Marisol Izquierdo
Original Article

Abstract

This study reports for the first time on European sea bass, Dicentrarchus labrax (L.), larvae, the effect of different levels of dietary arachidonic acid (ARA; 20:4n-6) on the expression of genes related to the fish stress response. Copies of mRNA from genes related to steroidogenesis [StAR (steroidogenic acute regulatory protein), c-Fos, and CYP11β (11β-hydroxylase gene)], glucocorticoid receptor complex [GR (glucocorticoid receptor) and HSP (heat shock proteins) 70 and 90) and antioxidative stress (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase] were quantified. Eighteen day-old larvae were fed for 14 days with three experimental diets with increasing levels of ARA (0.3, 0.6 and 1.2 % d.w.) and similar levels of docosahexaenoic (22:6n-3) and eicosapentaenoic (20:5n-3) acids (5 and 3 %, respectively). The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real time RT-PCR with the standard curve method (absolute quantification). Increase dietary levels of ARA induced a significantly (p < 0.05) down-regulation of genes related to cortisol synthesis, such as StAR and CYP11β and up-regulated genes related to glucocorticoid receptor complex, such as HSP70 and GR. No effects were observed on antioxidant enzymes gene expression. These results revealed the regulatory role of dietary ARA on the expression of stress-related genes in European sea bass larvae.

Keywords

Dicentrarchus labrax Arachidonic acid Stress HSP Glucocorticoid receptor 

Abbreviations

ACTH

Adrenocorticotropic hormone

ARA

Arachidonic acid (204n-6)

CAT

Catalase

COX

Cyclooxygenase

CYP11β

Cytochrome 11β-hydroxylase

DHA

Docosahexaenoic acid (226n-3)

EPA

Eicosapentaenoic acid (205n-3)

GH

Growth hormone

GLC

Gas liquid chromatography

GPX

Glutathione peroxidase

GR

Glucocorticoid receptor

HSP

Heat shock protein

HPI

Hypothalamic-pituitary-interrenal

IGF

Insulin growth factor

LC-PUFA

Long chain polyunsaturated fatty acid(s)

MW

Molecular weight

PIn

Peroxidation index

PGE

Prostaglandin E

RT-PCR

Real time polymerase chain reaction

SGR

Specific growth rate

SOD

Superoxide dismutase

StAR

Steroidogenic acute regulatory protein

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Copyright information

© AOCS 2015

Authors and Affiliations

  • Daniel Montero
    • 1
  • Genciana Terova
    • 2
  • Simona Rimoldi
    • 2
  • Mónica B. Betancor
    • 3
  • Eyad Atalah
    • 1
  • Silvia Torrecillas
    • 1
  • María J. Caballero
    • 1
  • María J. Zamorano
    • 1
  • Marisol Izquierdo
    • 1
  1. 1.Grupo de Investigación en Acuicultura (GIA), Parque Científico Tecnológico Marino de TaliarteUniversidad de Las Palmas de Gran Canaria (ULPGC)Las PalmasSpain
  2. 2.Department of Biotechnology and Life SciencesUniversity of InsubriaVareseItaly
  3. 3.Institute of Aquaculture, School of Natural SciencesUniversity of StirlingStirlingUK

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