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Lipids

, Volume 50, Issue 3, pp 227–239 | Cite as

miRNA Expression in Human Intestinal Caco-2 Cells is Comparably Regulated by cis- and trans-Fatty Acids

  • Solveigh Köpke
  • Thorsten BuhrkeEmail author
  • Alfonso Lampen
Original Article

Abstract

Trans-fatty acids are unsaturated fatty acids with at least one double bond in trans configuration. While their role in the development of coronary heart disease is broadly accepted, a potential impact of these fatty acids on colon carcinogenesis is still under discussion. MiRNAs are small non-coding RNAs that regulate the gene expression at a post-transcriptional level by inhibiting the translation of target mRNAs. We investigated the effect of 16 different C 18 fatty acid isomers on the expression of 84 cancer-related miRNAs in the human colorectal adenocarcinoma cell line Caco-2 by using a qRT-PCR array. 66 of these 84 miRNAs were deregulated by at least one fatty acid, however, there was no trans-specific impact on miRNA expression as the corresponding cis isomer of a given fatty acid generally had comparable effects on the miRNA expression profile. The most pronounced effects were observed for hsa-miR-146a-5p, which was upregulated by four of the 16 investigated fatty acids, and hsa-miR-32-5p, which was strongly downregulated by five fatty acids. As hsa-miR-32-5p was described to target genes being involved in the regulation of apoptosis, the effect of α-eleostearic acid on the expression of the apoptosis-associated genes BCL2L11, BCL-2, and BCL-XL was examined. The qPCR results indicate that fatty acid-mediated downregulation of hsa-miR-32-5p is accompanied by a downregulation of BCL-2 and BCL2L11 mRNA whereas BCL-XL was shown to be simultaneously upregulated. In conclusion, our data indicate that several fatty acids are able to regulate miRNA expression of human colon cancer cells. However, no trans-specific regulation was observed.

Keywords

miRNA trans-fatty acid CRC Colorectal cancer hsa-miR-32 Caco-2 Fatty acid TFA 

Abbreviations

Bcl-2

B-Cell lymphoma 2

BCL2L11

Bcl-2-like protein 11 (bim)

BCL-XL

B-cell lymphoma-extra large

BSA

Bovine serum albumin

CHD

Coronary heart disease

cDNA

Complementary DNA

CRC

Colorectal cancer

CTB

Cell titer blue™

DMEM

Dulbecco’s modified eagle medium

DMSO

Dimethylsulfoxide

FCS

Fetal calf serum

ITS

Insulin-transferrin-selenium supplement

miRNA

MicroRNA

mRNA

Messenger RNA

MMP16

Matrix metalloprotease 16

nt

Nucleotides

PTEN

Phosphatase and tensin homologue

qRT-PCR

Quantitative real time PCR

SCFA

Short chain fatty acid

snRNA

Small nuclear RNA

snoRNA

Small nucleolar RNA

SNP

Single nucleotide polymorphism

TFA

Trans fatty acid

wt

Wild type

Notes

Acknowledgments

S. K. has received a Grant from the Arthur and Aenne Feindt Foundation. We thank Gunnar Quer for technical assistance. This work was funded by the Federal Institute for Risk Assessment (Project no. 1322-474).

Conflicts of interest

The authors declare that there is no conflict of interest.

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Copyright information

© AOCS 2015

Authors and Affiliations

  • Solveigh Köpke
    • 1
  • Thorsten Buhrke
    • 1
    Email author
  • Alfonso Lampen
    • 1
  1. 1.Department of Food SafetyFederal Institute for Risk AssessmentBerlinGermany

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