, Volume 48, Issue 8, pp 803–815 | Cite as

Three Dissimilar High Fat Diets Differentially Regulate Lipid and Glucose Metabolism in Obesity-Resistant Slc:Wistar/ST Rats

  • Yoko Hashimoto
  • Kazuyo Yamada
  • Hiromi Tsushima
  • Daisuke Miyazawa
  • Mayumi Mori
  • Koji Nishio
  • Takeshi Ohkubo
  • Hidehiko Hibino
  • Naoki Ohara
  • Harumi Okuyama
Original Article


Epidemiologic and ecologic studies suggest that dietary fat plays an important role in the development of obesity. Certain Wistar rat strains do not become obese when fed high-fat diets unlike others. In a preliminary study, we confirmed that Slc:Wistar/ST rats did not become obese when fed high-fat diets. The mechanisms governing the response of hepatic lipid-metabolizing enzymes to large quantities of dietary lipids consumed by obesity-resistant animals are unknown. The aim of the present study is to examine how obesity-resistant animals metabolize various types of high-fat diets and why they do not become obese. For this purpose, male Slc:Wistar/ST rats were fed a control low-fat diet (LS) or a high-fat diet containing fish oil (HF), soybean oil (HS), or lard (HL) for 4 weeks. We observed their phenotypes and determined lipid profiles in plasma and liver as well as mRNA expression levels in liver of genes related to lipid and glucose metabolism using DNA microarray and quantitative reverse transcriptase polymerase chain analyses. The body weights of all dietary groups were similar due to isocaloric intakes, whereas the weight of white adipose tissues in the LS group was significantly lower. The HF diet lowered plasma lipid levels by accelerated lipolysis in the peroxisomes and suppressed levels of very-low-density lipoprotein (VLDL) secretion. The HS diet promoted hepatic lipid accumulation by suppressed lipolysis in the peroxisomes and normal levels of VLDL secretion. The lipid profiles of rats fed the LS or HL diet were similar. The HL diet accelerated lipid and glucose metabolism.


High-fat diet Soybean oil Fish oil Lard Plasma lipids Hepatic lipids Gene expression 



Docosahexaenoic acid (22:6n-3)


Eicosapentaenoic acid (20:5n-3)


Linoleic acid (18:2n-6)


Monounsaturated fatty acid(s)


Non-esterified fatty acid


Polyunsaturated fatty acid(s)


Reverse transcriptase-polymerase chain reaction


Sodium dodecyl sulfate-polyacrylamide gel electrophoresis


Saturated fatty acid(s)




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Copyright information

© AOCS 2013

Authors and Affiliations

  • Yoko Hashimoto
    • 1
  • Kazuyo Yamada
    • 2
  • Hiromi Tsushima
    • 2
  • Daisuke Miyazawa
    • 2
  • Mayumi Mori
    • 3
  • Koji Nishio
    • 4
  • Takeshi Ohkubo
    • 5
  • Hidehiko Hibino
    • 5
  • Naoki Ohara
    • 2
  • Harumi Okuyama
    • 2
  1. 1.Department of Biochemistry, School of DentistryAichi-Gakuin UniversityChikusa-kuJapan
  2. 2.Open Research CenterKinjo Gakuin University College of PharmacyMoriyama-kuJapan
  3. 3.Department of PharmacologyNagoya City University Graduate School of Medical ScienceMizuho-kuJapan
  4. 4.Department of Anatomy and NeuroscienceNagyoya University Graduate School of MedicineShowa-kuJapan
  5. 5.NOF CorporationKawasaki-kuJapan

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