, Volume 47, Issue 11, pp 1031–1041 | Cite as

Fish Oil Supplementation Reduces Cachexia and Tumor Growth While Improving Renal Function in Tumor-Bearing Rats

  • Isabela Coelho
  • Fernando Casare
  • Danielle C. T. Pequito
  • Gina Borghetti
  • Ricardo K. Yamazaki
  • Gleisson A. P. Brito
  • Marcelo Kryczyk
  • Luiz Claudio Fernandes
  • Terezila M. Coimbra
  • Ricardo FernandezEmail author
Original Article


The objective of the present work was to study the renal function of healthy and tumor-bearing rats chronically supplemented with fish oil (FO), a source of n-3 polyunsaturated fatty acids. Weanling male rats were divided in two groups, one control (C) and another orally supplemented for 70 days with FO (1 g/kg body weight). After this time, half the animals of each group were injected in the right flank with a suspension of Walker 256 tumor cells (W and WFO). The W group had less proteinemia reflecting cachectic proteolysis, FO reversed this fact. Tumor weight gain was also reduced in WFO. Glomerular filtration rate (GFR) was not different in FO or W compared to C, but was higher in WFO. Renal plasma flow (RPF) was higher in the FO supplemented groups. The W group had lower plasma osmolality than the C group, but FO supplementation resulted in normalization of this parameter. Fractional sodium excretion (FENa+) of FO rats was similar to C. Proximal Na+ reabsorption, evaluated by lithium clearance, was similar among the groups. Urinary thromboxane B2 (TXB2) excretion was lower in the supplemented groups. The number of macrophages in renal tissue was higher in W compared to C rats, but was lower in WFO rats compared to W rats. In conclusion, FO supplementation resulted in less tumor growth and cachexia, and appeared to be renoprotective, as suggested by higher RPF and GFR.


Fish oil supplementation Fatty acid Renal function Cachexia Glomerular filtration Sodium excretion 



Fish oil


Polyunsaturated fatty acid(s)


Eicosapentaenoic acid


Docosahexaenoic acid


Arachidonic acid






Arginine-vasopressin hormone


Control rats


Walker 256 tumor-bearing rats


Walker 256 tumor-bearing rats supplemented with fish oil


p-Aminohippuric acid


Renal plasma flow


Glomerular filtration rate


Fractional sodium excretion


Lithium clearance


Proximal fractional reabsorption of Na+


Post-proximal fractional excretion of Na+


Na+ distal delivery


Chemokine monocyte chemoattractant protein 1


Serum triacylglycerol





Authors thank Dr. Carolina A. Freire for the osmolality readings and useful suggestions. Research supported by Fundação Araucária, Paraná, Brasil. Luiz C. Fernandes and Terezila M. Coimbra were supported by grants of the Conselho Nacional de Pesquisas (CNPq), Brasil.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11745_2012_3715_MOESM1_ESM.doc (34 kb)
Supplementary material 1 (DOC 34 kb)


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Copyright information

© AOCS 2012

Authors and Affiliations

  • Isabela Coelho
    • 1
  • Fernando Casare
    • 1
  • Danielle C. T. Pequito
    • 1
  • Gina Borghetti
    • 1
  • Ricardo K. Yamazaki
    • 1
  • Gleisson A. P. Brito
    • 1
  • Marcelo Kryczyk
    • 1
  • Luiz Claudio Fernandes
    • 1
  • Terezila M. Coimbra
    • 2
  • Ricardo Fernandez
    • 1
    Email author
  1. 1.Departamento de Fisiologia, Setor de Ciências BiológicasUniversidade Federal do Paraná (UFPR) Centro Politécnico s/n.–Jd. das AméricasCuritibaBrazil
  2. 2.Departamento de Fisiologia–Faculdade de Medicina de Ribeirão PretoUniversidade de São Paulo (USP)Ribeirão PretoBrazil

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