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Lipids

, Volume 47, Issue 11, pp 1063–1071 | Cite as

Decreased Plasma Cholesterol Concentrations After PUFA-Rich Diets are not Due to Reduced Cholesterol Absorption/Synthesis

  • Vanu R. Ramprasath
  • Peter J. H. JonesEmail author
  • Donna D. Buckley
  • Laura A. Woollett
  • James E. Heubi
Original Article

Abstract

Plasma cholesterol concentrations increase with consumption of high saturated fatty acid (SFA) and decrease with high polyunsaturated fatty acid (PUFA) diets, leading to shifts in lipid levels consistent with reduction in heart disease risk. Direct measurements of cholesterol absorption, one of the key regulators of plasma cholesterol levels, have not been performed in humans after consumption of high PUFA diets. Thus, cholesterol absorption and fractional synthesis rates (FSRs) were measured in 16 healthy adults (8 males and 9 females) using a randomized cross-over study with a diet containing high (PUFA/SFA) P/S ratio (2:1) and a low P/S ratio (0.5:1). Cholesterol absorption and fractional cholesterol synthetic rates were measured using stable isotopes after 20 days of dietary intervention. Diet did not affect cholesterol absorption or synthesis. There was a significant decrease in plasma cholesterol concentrations (P < 0.02), specifically LDL-cholesterol (P < 0.02), without a change in HDL-cholesterol or triacylglycerol concentrations. Intraluminal cholesterol solubilization and plasma sterol (cholesterol biosynthetic intermediates and plant sterols) levels were not affected by diet. Thus, consumption of diets with a high P/S ratio reduces plasma total and LDL-cholesterol concentrations independent of shifts in cholesterol absorption or synthesis.

Keywords

Fatty acid Metabolic syndrome Cholesterol Bile Human PUFA SFA 

Abbreviations

PUFA

Polyunsaturated fatty acid(s)

P/S

Ratio between polyunsaturated fatty acid and saturated fatty acid

LDL

Low density lipoprotein

HDL

High density lipoprotein

SFA

Saturated fatty acid(s)

CVD

Cardiovascular disease

RBC

Red blood cell

CCHMC

Cincinnati children’s hospital medical center

GCRC

General clinical research center

FSR

Fractional synthetic rate

GLC

Gas liquid chromatography

Notes

Acknowledgments

We thank YenMing Chan, Richardson Centre for Functional Foods and Nutraceuticals, and Dr. Scot Harding, adjunct professor, Department of Human Nutritional Sciences, University of Manitoba for their help with the analytical part of the study. We also thank Suzanne Summer and the staff of the Body Composition Core of the General Clinical Research Center for their help with dietary management for the study. We were also supported by the National Institute of Health RR 08084 and DK 068463.

Conflict of interest

No authors have any conflict of interest with the current study.

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Copyright information

© AOCS 2012

Authors and Affiliations

  • Vanu R. Ramprasath
    • 1
  • Peter J. H. Jones
    • 1
    Email author
  • Donna D. Buckley
    • 2
    • 3
    • 4
  • Laura A. Woollett
    • 5
  • James E. Heubi
    • 2
    • 3
    • 4
  1. 1.Richardson Centre for Functional Foods and NutraceuticalsUniversity of ManitobaWinnipegCanada
  2. 2.Division of Pediatric Gastroenterology/Hepatology and NutritionChildren’s Hospital Medical CenterCincinnatiUSA
  3. 3.Department of PediatricsChildren’s Hospital Medical CenterCincinnatiUSA
  4. 4.Clinical/Translational Research CenterChildren’s Hospital Medical CenterCincinnatiUSA
  5. 5.Department of Pathology and Laboratory MedicineUniversity of Cincinnati Medical SchoolCincinnatiUSA

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