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Lipids

, Volume 47, Issue 9, pp 873–880 | Cite as

Macrophages Alter the Differentiation-Dependent Decreases in Fibronectin and Collagen I/III Protein Levels in Human Preadipocytes

  • AnneMarie Gagnon
  • Michelle N. Yarmo
  • Anne Landry
  • Alexander SoriskyEmail author
Original Article

Abstract

Adipose tissue of obese individuals is characterized by increased fibrosis and macrophage infiltration. Extensive remodeling of the extracellular matrix (ECM) that occurs during adipogenesis can be influenced by macrophages, but it remains unclear how macrophage-secreted factors alter preadipocyte ECM protein expression under non-adipogenic versus adipogenic conditions. Confluent human subcutaneous abdominal preadipocytes were cultured for 14 days, with or without adipogenic inducers, in either control medium, medium conditioned by THP-1 monocytes (THP-1-MonCM), or medium conditioned by THP-1 macrophages (THP-1-MacCM). Under non-adipogenic conditions in THP-1-MacCM, collagen I/III and fibronectin protein levels rose by 40 and 70 %, respectively (p < 0.05, n = 3; compared to control non-adipogenic medium). When preadipocytes were exposed to adipogenic inducers in THP-1-MacCM, collagen I/III levels increased by 50 %, but those of fibronectin fell by 48 %, both compared to non-adipogenic THP-1-MacCM conditions. The rise in collagen I/III levels contrasts with the 51 % decrease in collagen I/III that occurs with induction of differentiation in control medium, whereas, the decrease in fibronectin is more modest, but consistent in THP-1-MacCM (48 %) and control medium (92 %). A similar effect on fibronectin levels occurred using medium conditioned by LPS-treated human monocyte-derived macrophages (MD-MacCM). Our data indicate macrophage-derived factors regulate levels of collagen I/III and fibronectin in preadipocytes under non-adipogenic and adipogenic conditions. Further studies are needed to determine if these changes in these ECM proteins contribute to the anti-adipogenic action of MacCM.

Keywords

Preadipocyte Adipogenesis Fibronectin Collagen Extracellular matrix Macrophage 

Abbreviations

BMI

Body mass index

ECM

Extracellular matrix

ERK1/2

Extracellular signal-regulated kinase 1/2

FABP4/aP2

Fatty acid binding protein 4

IL-1β

Interleukin-1β

IOD

Integrated optical density

LPS

Lipopolysaccharide

MacCM

Macrophage-conditioned medium

MMP

Matrix metalloproteinase

MD

Monocyte-derived

MonCM

Monocyte-conditioned medium

PPARγ

Peroxisome proliferator-activated receptor γ

TG

Triacylglycerol

TIMP

Tissue inhibitor of matrix metalloproteinase

TNFα

Tumor necrosis factor α

TPA

12-O-Tetradecanoylphorbol-13-acetate

Notes

Acknowledgments

This work was supported by a grant to AS from the Heart and Stroke Foundation of Canada (Ontario) NA 6634. We thank Fiona Frappier and Dr. J B Angel (OHRI) for assistance with production of MD-MacCM, and patients and surgeons of The Ottawa Hospital for their participation in acquiring adipose tissue samples.

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Copyright information

© AOCS 2012

Authors and Affiliations

  • AnneMarie Gagnon
    • 1
  • Michelle N. Yarmo
    • 1
  • Anne Landry
    • 1
  • Alexander Sorisky
    • 1
    Email author
  1. 1.Chronic Disease Program, Ottawa Hospital Research Institute, Departments of Medicine and of Biochemistry, Microbiology and ImmunologyUniversity of OttawaOttawaCanada

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