An Egg-Enriched Diet Attenuates Plasma Lipids and Mediates Cholesterol Metabolism of High-Cholesterol Fed Rats
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We investigated the influence of an egg-enriched diet on plasma, hepatic and fecal lipid levels and on gene expression levels of transporters, receptors and enzymes involved in cholesterol metabolism. Sprague–Dawley rats fed an egg-enriched diet had lower plasma triglycerides, total cholesterol, low density lipoprotein (LDL)-cholesterol, hepatic triglyceride, and cholesterol concentrations, and greater plasma high-density lipoprotein cholesterol concentration, fecal neutral sterol and bile acid concentrations than those fed a plain cholesterol diet. Chicken egg yolk had no effect on sterol 12α-hydroxylase and sterol 27α-hydroxylase; but upregulated mRNA levels of hepatic LDL-receptor, cholesterol 7α-hydroxylase (CYP7A1) and lecithin cholesterol acyltransferase, and downregulated hepatic hydroxymethylglutaryl-(HMG)-CoA reductase and acyl-CoA:cholesterol acyltransferase (ACAT) after 90 days. Modification of the lipoprotein profile by an egg-enriched diet was mediated by reducing de novo cholesterol synthesis and enhancing the excretion of fecal cholesterol, via upregulation of CYP7A1 and the LDL receptor, and downregulation of HMG-CoA reductase and ACAT.
KeywordsEgg-enriched diet Cholesterol Plasma lipids mRNA expression
Spontaneously hypertensive rats
Real time quantitative polymerase chain reaction
Common control group
Egg yolk group
Whole egg group
Total bile acid
Hematoxylin and eosin
- HMG-CoA R
Hydroxymethylglutaryl CoA reductase
Lecithin cholesterol acyltransferase
Egg white protein
We duly acknowledge the financial support received from the earmarked fund for Modern Agro-industry Technology Research System (Project Code No.: nycytx-41-g22).
Conflict of interest
All authors declare that there are no conflicts of interest.
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