, Volume 47, Issue 3, pp 269–277 | Cite as

An Egg-Enriched Diet Attenuates Plasma Lipids and Mediates Cholesterol Metabolism of High-Cholesterol Fed Rats

Original Article


We investigated the influence of an egg-enriched diet on plasma, hepatic and fecal lipid levels and on gene expression levels of transporters, receptors and enzymes involved in cholesterol metabolism. Sprague–Dawley rats fed an egg-enriched diet had lower plasma triglycerides, total cholesterol, low density lipoprotein (LDL)-cholesterol, hepatic triglyceride, and cholesterol concentrations, and greater plasma high-density lipoprotein cholesterol concentration, fecal neutral sterol and bile acid concentrations than those fed a plain cholesterol diet. Chicken egg yolk had no effect on sterol 12α-hydroxylase and sterol 27α-hydroxylase; but upregulated mRNA levels of hepatic LDL-receptor, cholesterol 7α-hydroxylase (CYP7A1) and lecithin cholesterol acyltransferase, and downregulated hepatic hydroxymethylglutaryl-(HMG)-CoA reductase and acyl-CoA:cholesterol acyltransferase (ACAT) after 90 days. Modification of the lipoprotein profile by an egg-enriched diet was mediated by reducing de novo cholesterol synthesis and enhancing the excretion of fecal cholesterol, via upregulation of CYP7A1 and the LDL receptor, and downregulation of HMG-CoA reductase and ACAT.


Egg-enriched diet Cholesterol Plasma lipids mRNA expression 



Cardiovascular disease






Spontaneously hypertensive rats


Real time quantitative polymerase chain reaction




Common control group


Cholesterol group


Egg yolk group


Whole egg group


Total bile acid






Hematoxylin and eosin


Glyceraldehyde-3-phosphate dehydrogenase


Hydroxymethylglutaryl CoA reductase


LDL receptor


Cholesterol 7α-hydroxylase


Sterol 27α-hydroxylase


Sterol 12α-hydroxylase


Acyl-CoA:cholesterol acyltransferase


Lecithin cholesterol acyltransferase


Egg white protein



We duly acknowledge the financial support received from the earmarked fund for Modern Agro-industry Technology Research System (Project Code No.: nycytx-41-g22).

Conflict of interest

All authors declare that there are no conflicts of interest.


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Copyright information

© AOCS 2012

Authors and Affiliations

  • Fang Yang
    • 1
  • Meihu Ma
    • 1
  • Jia Xu
    • 2
  • Xiufang Yu
    • 1
  • Ning Qiu
    • 1
  1. 1.National R&D Center for Egg Processing, College of Food Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of Animal Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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