Lipids

, Volume 46, Issue 9, pp 805–811

Ethanolic Extract of Propolis Promotes Reverse Cholesterol Transport and the Expression of ATP-Binding Cassette Transporter A1 and G1 in Mice

  • Yang Yu
  • Yanhong Si
  • Guohua Song
  • Tian Luo
  • Jiafu Wang
  • Shucun Qin
Original Article

Abstract

The ethanolic extract of propolis (EEP) is beneficial in increasing high density lipoprotein (HDL) cholesterol (HDL-C) and diminishing risks of atherosclerosis. In this study, we examined the effects of EEP on reverse cholesterol transport in mice. 3H -cholesterol laden macrophage was injected intraperitoneally into mice fed by gastric gavage with EEP. Plasma lipid level was determined and 3H-cholesterol was traced in plasma, liver and feces. The effects of EEP on ATP-binding cassette transporter A1 and G1 (ABCA1 and ABCG1) and scavenger receptor BI (SR-BI) in mice liver and in cultured cells were also investigated. EEP administration led to a significant increase in HDL-C and peritoneal macrophage-original 3H-cholesterol in plasma, liver and feces. Liver protein expressions of ABCA1 and ABCG1 were increased but SR-B1 was not. In vitro experiments with HepG2 and Raw264.7 cell lines confirmed the above results. The finding of these studies shows that EEP-enhanced reverse cholesterol transport may have resulted from EEP stimulated plasma HDL level and hepatic ABCA1 and ABCG1 expression.

Keywords

Ethanolic extract of propolis Reverse cholesterol transport High density lipoprotein ATP-binding cassette transporters A1 ATP-binding cassette transporters G1 Scavenger receptor BI 

Abbreviations

ABCA1

ATP-binding cassette transporter A1

ABCG1

ATP-binding cassette transporter G1

Ac-LDL

Acetylated low density lipoprotein

ANOVA

Analysis of variance

CPM

Count/min

DMEM

Dulbecco’s modified eagle medium

DMSO

Dimethyl sulfoxide

EEP

Ethanolic extract of propolis

FBS

Fetal bovine serum

HDL

High density lipoprotein

HDL-C

High density lipoprotein cholesterol

LDL

Low density lipoprotein

LDL-C

Low density lipoprotein cholesterol

PVDF

Polyvinylidene fluoride

RCT

Reverse cholesterol transport

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SR-BI

Scavenger receptor BI

TC

Total cholesterol

TG

Triglyceride(s)

References

  1. 1.
    Lewis GF, Rader DJ (2005) New insights into the regulation of HDL metabolism and reverse cholesterol transport. Circ Res 96(12):1221–1232PubMedCrossRefGoogle Scholar
  2. 2.
    Oram JF, Vaughan AM (2006) ATP-binding cassette cholesterol transporters and cardiovascular disease. Circ Res 99(10):1031–1043PubMedCrossRefGoogle Scholar
  3. 3.
    Wang X et al (2007) Macrophage ABCA1 and ABCG1, but not SR-BI, promote macrophage reverse cholesterol transport in vivo. J Clin Invest 117(8):2216–2224PubMedCrossRefGoogle Scholar
  4. 4.
    Cavelier C, Lorenzi I, Rohrer L, von Eckardstein A (2006) Lipid efflux by the ATP-binding cassette transporters ABCA1 and ABCG1. Biochim Biophys Acta 1761(7):655–666PubMedGoogle Scholar
  5. 5.
    Volpert R, Elstner EF (1993) Biochemical activities of propolis extracts I Standardization and antioxidative properties of ethanolic and aqueous derivatives. Z Naturforsch C 48(11–12):851–857PubMedGoogle Scholar
  6. 6.
    Nader MA, el-Agamy DS, Suddek GM (2010) Protective effects of propolis and thymoquinone on development of atherosclerosis in cholesterol-fed rabbits. Arch Pharm Res 33(4):637–643PubMedCrossRefGoogle Scholar
  7. 7.
    Newairy AS, Salama AF, Hussien HM, Yousef MI (2009) Propolis alleviates aluminium-induced lipid peroxidation and biochemical parameters in male rats. Food Chem Toxicol 47(6):1093–1098PubMedCrossRefGoogle Scholar
  8. 8.
    El-Sayed el SM, Abo-Salem OM, Aly HA, Mansour AM (2009) Potential antidiabetic and hypolipidemic effects of propolis extract in streptozotocin-induced diabetic rats. Pak J Pharm Sci 22(2):168–174Google Scholar
  9. 9.
    Si Y-H et al (2005) Interventional effect of propolis on atherosclerosis induced by hyperlipid in rabbits. Chin J Clin Rehabil 9(35):82–84Google Scholar
  10. 10.
    Koya-Miyata S et al (2009) Propolis prevents diet-induced hyperlipidemia and mitigates weight gain in diet-induced obesity in mice. Biol Pharm Bull 32(12):2022–2028PubMedCrossRefGoogle Scholar
  11. 11.
    Miyazaki A et al (1994) Acetylated low density lipoprotein reduces its ligand activity for the scavenger receptor after interaction with reconstituted high density lipoprotein. J Biol Chem 269(7):5264–5269PubMedGoogle Scholar
  12. 12.
    Moore RE et al (2005) Increased atherosclerosis in mice lacking apolipoprotein A-I attributable to both impaired reverse cholesterol transport and increased inflammation. Circ Res 97(8):763–771PubMedCrossRefGoogle Scholar
  13. 13.
    Naik SU et al (2006) Pharmacological activation of liver X receptors promotes reverse cholesterol transport in vivo. Circulation 113(1):5–8Google Scholar
  14. 14.
    Owen JS, Mulcahy JV (2002) ATP-binding cassette A1 protein and HDL homeostasis. Atheroscler Suppl 3(4):13–22PubMedCrossRefGoogle Scholar
  15. 15.
    Xia M et al (2005) Anthocyanins induce cholesterol efflux from mouse peritoneal macrophages: the role of the peroxisome proliferator-activated receptor {gamma}-liver X receptor {alpha}-ABCA1 pathway. J Biol Chem 280(44):36792–36801PubMedCrossRefGoogle Scholar
  16. 16.
    Terra X et al (2009) Inhibitory effects of grape seed procyanidins on foam cell formation in vitro. J Agric Food Chem 57(6):2588–2594PubMedCrossRefGoogle Scholar
  17. 17.
    Wiersma H et al (2009) Lack of Abcg1 results in decreased plasma HDL cholesterol levels and increased biliary cholesterol secretion in mice fed a high cholesterol diet. Atherosclerosis 206(1):141–147PubMedCrossRefGoogle Scholar
  18. 18.
    Uto-Kondo H et al (2010) Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages. Circ Res 106(4):779–787PubMedCrossRefGoogle Scholar

Copyright information

© AOCS 2011

Authors and Affiliations

  • Yang Yu
    • 1
  • Yanhong Si
    • 2
  • Guohua Song
    • 1
  • Tian Luo
    • 1
  • Jiafu Wang
    • 2
  • Shucun Qin
    • 1
    • 2
  1. 1.Institute of AtherosclerosisTaishan Medical UniversityTaianPeople’s Republic of China
  2. 2.Department of Basic MedicineTaishan Medical UniversityTaianPeople’s Republic of China

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