, Volume 46, Issue 9, pp 805–811 | Cite as

Ethanolic Extract of Propolis Promotes Reverse Cholesterol Transport and the Expression of ATP-Binding Cassette Transporter A1 and G1 in Mice

  • Yang Yu
  • Yanhong Si
  • Guohua Song
  • Tian Luo
  • Jiafu Wang
  • Shucun Qin
Original Article


The ethanolic extract of propolis (EEP) is beneficial in increasing high density lipoprotein (HDL) cholesterol (HDL-C) and diminishing risks of atherosclerosis. In this study, we examined the effects of EEP on reverse cholesterol transport in mice. 3H -cholesterol laden macrophage was injected intraperitoneally into mice fed by gastric gavage with EEP. Plasma lipid level was determined and 3H-cholesterol was traced in plasma, liver and feces. The effects of EEP on ATP-binding cassette transporter A1 and G1 (ABCA1 and ABCG1) and scavenger receptor BI (SR-BI) in mice liver and in cultured cells were also investigated. EEP administration led to a significant increase in HDL-C and peritoneal macrophage-original 3H-cholesterol in plasma, liver and feces. Liver protein expressions of ABCA1 and ABCG1 were increased but SR-B1 was not. In vitro experiments with HepG2 and Raw264.7 cell lines confirmed the above results. The finding of these studies shows that EEP-enhanced reverse cholesterol transport may have resulted from EEP stimulated plasma HDL level and hepatic ABCA1 and ABCG1 expression.


Ethanolic extract of propolis Reverse cholesterol transport High density lipoprotein ATP-binding cassette transporters A1 ATP-binding cassette transporters G1 Scavenger receptor BI 



ATP-binding cassette transporter A1


ATP-binding cassette transporter G1


Acetylated low density lipoprotein


Analysis of variance




Dulbecco’s modified eagle medium


Dimethyl sulfoxide


Ethanolic extract of propolis


Fetal bovine serum


High density lipoprotein


High density lipoprotein cholesterol


Low density lipoprotein


Low density lipoprotein cholesterol


Polyvinylidene fluoride


Reverse cholesterol transport


Sodium dodecyl sulfate polyacrylamide gel electrophoresis


Scavenger receptor BI


Total cholesterol





This study was supported by a grant from the “Tai Shan Scholars” foundation of Shandong province (ZD056, ZD057), and by a special foundation of Tai Shan Medical College (1065). We would like to thank Guanghai Zhou and Xinnong Wang for expert technical assistance.


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Copyright information

© AOCS 2011

Authors and Affiliations

  • Yang Yu
    • 1
  • Yanhong Si
    • 2
  • Guohua Song
    • 1
  • Tian Luo
    • 1
  • Jiafu Wang
    • 2
  • Shucun Qin
    • 1
    • 2
  1. 1.Institute of AtherosclerosisTaishan Medical UniversityTaianPeople’s Republic of China
  2. 2.Department of Basic MedicineTaishan Medical UniversityTaianPeople’s Republic of China

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