, Volume 46, Issue 7, pp 617–625

Aerobic Exercise Improves Reverse Cholesterol Transport in Cholesteryl Ester Transfer Protein Transgenic Mice

  • D. D. F. M. Rocco
  • L. S. Okuda
  • R. S. Pinto
  • F. D. Ferreira
  • S. K. Kubo
  • E. R. Nakandakare
  • E. C. R. Quintão
  • S. Catanozi
  • M. Passarelli
Original Article


We analyzed the effect of a 6-week aerobic exercise training program on the in vivo macrophage reverse cholesterol transport (RCT) in human cholesteryl ester transfer protein (CETP) transgenic (CETP-tg) mice. Male CETP-tg mice were randomly assigned to a sedentary group or a carefully supervised exercise training group (treadmill 15 m/min, 30 min sessions, five sessions per week). The levels of plasma lipids were determined by enzymatic methods, and the lipoprotein profile was determined by fast protein liquid chromatography (FPLC). CETP activity was determined by measuring the transfer rate of 14C-cholesterol from HDL to apo-B containing lipoproteins, using plasma from CETP-tg mice as a source of CETP. The reverse cholesterol transport was determined in vivo by measuring the [3H]-cholesterol recovery in plasma and feces (24 and 48 h) and in the liver (48 h) following a peritoneal injection of [3H]-cholesterol labeled J774-macrophages into both sedentary and exercise trained mice. The protein levels of liver receptors were determined by immunoblot, and the mRNA levels for liver enzymes were measured using RT-PCR. Exercise training did not significantly affect the levels of plasma lipids or CETP activity. The HDL fraction assessed by FPLC was higher in exercise-trained compared to sedentary mice. In comparison to the sedentary group, a greater recovery of [3H]-cholesterol from the injected macrophages was found in the plasma, liver and feces of exercise-trained animals. The latter occurred even with a reduction in the liver CYP7A1 mRNA level in exercised trained animals. Exercise training increased the liver LDL receptor and ABCA-1 protein levels, although the SR-BI protein content was unchanged. The RCT benefit in CETP-tg mice elicited by exercise training helps to elucidate the role of exercise in the prevention of atherosclerosis in humans.


Atherosclerosis CETP HDL Physical exercise Cholesterol 



Transporters ABCA-1 and ABCG-1


Acetylated LDL

Apo AI

Apolipoprotein AI

Apo E

Apolipoprotein E


Low density lipoprotein receptor


Cholesteryl ester transfer protein


7 alpha hydroxylase


27 alpha hydroxylase


Esterified cholesterol


Ethylene diamine tetra acetic phosphate-buffered saline


Fast protein liquid chromatography


High density lipoprotein


Lecithin cholesterol acyltransferase


Low density lipoprotein


LDL-receptor related protein


Liver X receptor




Reverse cholesterol transport


Scavenger receptor class B type I


Very low density lipoprotein


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Copyright information

© AOCS 2011

Authors and Affiliations

  • D. D. F. M. Rocco
    • 1
  • L. S. Okuda
    • 1
  • R. S. Pinto
    • 1
  • F. D. Ferreira
    • 1
  • S. K. Kubo
    • 2
  • E. R. Nakandakare
    • 1
  • E. C. R. Quintão
    • 1
  • S. Catanozi
    • 1
  • M. Passarelli
    • 1
  1. 1.Lipids Laboratory (LIM-10), Faculty of Medical SciencesUniversity of Sao PauloSao PauloBrazil
  2. 2.Emergency Care Research Unit (LIM-51), Faculty of Medical SciencesUniversity of Sao PauloSao PauloBrazil

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