Lipids

, Volume 46, Issue 6, pp 487–494

Temporary Increase of PPAR-γ and Transient Expression of UCP-1 in Stromal Vascular Fraction Isolated Human Adipocyte Derived Stem Cells During Adipogenesis

  • Seong Jin Jo
  • Won Woo Choi
  • Eun Seong Lee
  • Jae Yong Lee
  • Hyun Sun Park
  • Dae Won Moon
  • Hee Chul Eun
  • Jin Ho Chung
Original Article

Abstract

In this study, cells from the stromal vascular fraction of human subcutaneous tissues were induced to differentiate toward adipose cells in vitro for 2 weeks. During adipogenic differentiation, we followed the chronological changes in their morphology with Coherent anti-Stokes Raman scattering (CARS) microscopy and checked the PPAR-γ and UCP-1 expression with RT-PCR. On day 4 after inducing adipogenic differentiation, CARS imaging showed multiple small lipid droplets (LD) distributed peripherally along the cellular membrane. PPAR-γ began to express at this time and increased until day 14 at a steady rate. On day 7, the cells appeared as brown adipocytes with numerous small LD throughout the cytoplasm, and the mRNA level of UCP-1 rose abruptly by 6- to 7-fold. After an additional 7 days, CARS imaging showed the development of a large LD, which is characteristic of white adipocytes, and the mRNA level of UCP-1 slumped significantly. These results demonstrate the possibility that ADSC pass through a brown adipocyte-like stage while differentiating into white adipocytes.

Keywords

Adipogenesis Adipose-derived stem cell UCP-1 

Abbreviations

ADSC

Adipose-derived stem cell

BAT

Brown adipose tissue

CARS

Coherent anti-Stokes Raman scattering

DMEM

Dulbecco’s modified Eagle’s medium

FBS

Fetal bovine serum

IBMX

Isobutyl-methylxanthine

LD

Lipid droplet

PBS

Phosphate-buffered saline

PPAR-γ

Peroxisome proliferator-activated receptor-γ

SVF

Stromal vascular fraction

UCP-1

Mitochondria-uncoupling protein-1

WAT

White adipose tissue

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Copyright information

© AOCS 2011

Authors and Affiliations

  • Seong Jin Jo
    • 1
  • Won Woo Choi
    • 2
  • Eun Seong Lee
    • 3
  • Jae Yong Lee
    • 3
  • Hyun Sun Park
    • 1
  • Dae Won Moon
    • 3
  • Hee Chul Eun
    • 1
  • Jin Ho Chung
    • 1
  1. 1.Department of DermatologySeoul National University College of MedicineSeoulKorea
  2. 2.Wells Dermatology ClinicSeoulKorea
  3. 3.Center for NanoBio ConvergenceKorea Research Institute of Standards and ScienceDaejeonKorea

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