, Volume 46, Issue 2, pp 189–199 | Cite as

Insulin Stimulates Lipogenesis and Attenuates Beta-Oxidation in White Adipose Tissue of Fed Rainbow Trout

  • S. PolakofEmail author
  • F. Médale
  • L. Larroquet
  • C. Vachot
  • G. Corraze
  • S. Panserat
Original Article


As lipid deposition tissue in fish, the white adipose tissue (WAT) has important functions related to reproduction and the challenges of long-term fasting. In the study reported here, we infused fish fed a high-carbohydrate diet with two doses of insulin for 5 days in order to explore the effects of this hormone on lipogenesis and beta-oxidation-related enzymes. We demonstrated the presence of some of the main lipogenic enzymes at molecular, protein and activity levels (ATP-citrate lyase and fatty acid synthase). However, while ATP-citrate lyase was unexpectedly down-regulated, fatty acid synthase was up-regulated (at protein and activity levels) in an insulin dose-dependent manner. The main enzymes acting as NADPH donors for lipogenesis were also characterized at biochemical and molecular levels, although there was no evidence of their regulation by insulin. On the other hand, lipid oxidation potential was found in this tissue through the measurement of gene expression of enzymes involved in β-oxidation, highlighting two carnitine palmitoyltransferase isoforms, both down-regulated by insulin infusion. We found that insulin acts as an important regulator of trout WAT lipid metabolism, inducing the final stage of lipogenesis at molecular, protein and enzyme activity levels and suppressing β-oxidation at least at a molecular level. These results suggest that WAT in fish may have a role that is important not only as a lipid deposition tissue but also as a lipogenic organ (with possible involvement in glucose homeostasis) that could also be able to utilize the lipids stored as a local energy source.


Insulin Fish Dietary carbohydrates White adipose tissue Lipogenesis Lipid oxidation 



6-Phosphogluconate dehydrogenase


ATP citrate lyase


Carnitine palmitoyltransferase


Elongation factor 1 alpha


Free fatty acids


Glucose 6-phosphate dehydrogenase


3-Hydroxyacyl-CoA dehydrogenase


Hormone-sensitive lipase


Isocitrate dehydrogenase


Lipoprotein lipase


Malic enzyme


Nicotine adenine dinucleotide phosphate, reduced




Tumor necrosis factor-alpha


White adipose tissue



This study was supported by research grants from the Agence Nationale de la Recherche (ANR-08-JCJC-0025-01) and INRA PHASE Department. SP was recipient of a postdoctoral fellowship from the Xunta de Galicia (Program Ángeles Alvariño). We thank the technical stuff (Y. Hontang, F. Sandres, and F. Terrier) of the INRA experimental fish farm of Donzacq for supplying the experimental animals.


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Copyright information

© AOCS 2011

Authors and Affiliations

  • S. Polakof
    • 1
    • 2
    Email author
  • F. Médale
    • 1
  • L. Larroquet
    • 1
  • C. Vachot
    • 1
  • G. Corraze
    • 1
  • S. Panserat
    • 1
  1. 1.INRA, UMR1067 Nutrition Aquaculture et Génomique, Pôle d’hydrobiologie, CD918Saint-Pée-sur-NivelleFrance
  2. 2.Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da SaúdeFacultade de Bioloxía, Universidade de VigoVigoSpain

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