Lipids

, Volume 46, Issue 1, pp 47–57 | Cite as

A High Omega-3 Fatty Acid Diet has Different Effects on Early and Late Stage Myeloid Progenitors

  • Melinda E. Varney
  • James T. Buchanan
  • Yulia Dementieva
  • W. Elaine Hardman
  • Vincent E. Sollars
Original Article

Abstract

The effects of the polyunsaturated omega-3 (n-3) and omega-6 (n-6) fatty acids (FA) on hematopoiesis are complex in that both FA forms are processed into leukotrienes, eicosanoids, and prostaglandins, which can have independent effects. These FA have antagonistic effects in that n-6 FA prostaglandins tend to be pro-proliferative and pro-inflammatory, while the effects of n-3 FA prostaglandins are the opposite. We have previously shown that diets high in n-3 FA reduce the size of the middle to later stage myeloid progenitor compartment in FVB X sv129 F1hybrid mice. To assay the effects of high n-3 FA diets on earlier stages of myelopoiesis, we fed C57BL/6J mice diets high in n-3 FA or levels of n-3/n-6 FA similar to western diets and assayed the effects on myelopoiesis with flow cytometry and colony forming cell assays. Results indicate an expansion of the common myeloid progenitor cell compartment in high n-3 FA diets, which does not persist into later stages where the number of progenitor cells is actually lower in high n-3 FA fed animals. Investigations in vitro with the hematopoietic stem cell line EML-clone 1 indicate that cells cultured with eicosapentaenoic acid (n-3 FA) or arachidonic acid (n-6 FA) have no differences in cell viability but that arachidonic acid more rapidly produces progenitors with low levels of the macrophage developmental marker, F4/80.

Keywords

Omega fatty acids EPA DHA Immunology Stem cells Progenitor cells Bone marrow Nutrition 

Abbreviations

n-3

Omega-3

n-6

Omega-6

ARA

Arachidonic acid

ALA

α Linolenic acid

AIN-76A diet

American institute of nutrition 76A diet

APC

Allophycocyanin

ATRA

all-trans Retinoic acid

BHK

Baby hamster kidney

CFC

Colony forming cell(s)

CFU-GM

Colony forming unit granulocyte–macrophage

CFU-M

Colony forming unit macrophage

CMP

Common myeloid progenitor(s)

COX

Cyclooxygenase

DHAn-3

Docosahexaenoic acid

DPA

Docosapentaenoic acid

EDTA

Ethylenediaminetetraacetic acid

EML

Erythroid myeloid lymphoid

EPA

Eicosapentaenoic acid

FA

Fatty acid(s)

FC

Fragment crystallizable receptor γ

FSC

Forward scatter

GMP

Granulocyte–macrophage progenitor(s)

HSC

Hematopoietic stem cell(s)

IL-3

Interleukin-3

IL-7Rα

Interleukin-7 receptor α

LNA

Linoleic acid

MEP

Megakaryocyte–erythrocyte progenitor

PE

Phycoerythrin

PBS

Phosphate-buffered saline

SCF

Stem cell factor

SSC

Side scatter

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Copyright information

© AOCS 2010

Authors and Affiliations

  • Melinda E. Varney
    • 1
  • James T. Buchanan
    • 1
  • Yulia Dementieva
    • 2
  • W. Elaine Hardman
    • 1
  • Vincent E. Sollars
    • 1
  1. 1.Department of Biochemistry and MicrobiologyMarshall University School of MedicineHuntingtonUSA
  2. 2.Department of MathematicsEmmanuel CollegeBostonUSA

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