Lipids

, Volume 45, Issue 9, pp 863–875 | Cite as

Lipid Profiling Reveals Tissue-Specific Differences for Ethanolamide Lipids in Mice Lacking Fatty Acid Amide Hydrolase

  • Aruna Kilaru
  • Giorgis Isaac
  • Pamela Tamura
  • David Baxter
  • Scott R. Duncan
  • Barney J. Venables
  • Ruth Welti
  • Peter Koulen
  • Kent D. Chapman
Original Article

Abstract

N-Acylethanolamines (NAE) are fatty acid derivatives, some of which function as endocannabinoids in mammals. NAE metabolism involves common (phosphatidylethanolamines, PEs) and uncommon (N-acylphosphatidylethanolamines, NAPEs) membrane phospholipids. Here we have identified and quantified more than a hundred metabolites in the NAE/endocannabinoid pathway in mouse brain and heart tissues, including many previously unreported molecular species of NAPE. We found that brain tissue of mice lacking fatty acid amide hydrolase (FAAH−/−) had elevated PE and NAPE molecular species in addition to elevated NAEs, suggesting that FAAH activity participates in the overall regulation of this pathway. This perturbation of the NAE pathway in brain was not observed in heart tissue of FAAH−/− mice, indicating that metabolic regulation of the NAE pathway differs in these two organs and the metabolic enzymes that catabolize NAEs are most likely differentially distributed and/or regulated. Targeted lipidomics analysis, like that presented here, will continue to provide important insights into cellular lipid signaling networks.

Keywords

FAAH Endocannabinoids N-Acylethanolamines Lipid profiling Lipid signaling Lipid mediators Mass spectrometry 

Abbreviations

AG

Arachidonoylglycerol

ePC

Alk(en)yl,acyl glycerophosphocholine

ePE

Alk(en)yl,acyl glycerophosphoethanolamine

ESI

Electrospray ionization

FAAH

Fatty acid amide hydrolase

FFA

Free fatty acid

FW

Fresh weight

KO

Knockout

LPC

Lysophosphatidylcholine

LPE

Lysophosphatidylethanolamine

NAE

N-Acylethanolamine

NAPE

N-Acylphosphatidylethanolamine

NL

Neutral loss

PA

Phosphatidic acid

PC

Phosphatidylcholine

PE

Phosphatidylethanolamine

PI

Phosphatidylinositol

PLD

Phospholipase D

Pre

Precursor

PS

Phosphatidylserine

SM

Sphingomyelin

WT

Wild type

X:Y

Designates carbon chain length: total number of carbon–carbon double bonds

Notes

Acknowledgments

We would like to thank Mary R. Roth for expert technical assistance. This work was supported by a seed grant from the University of North Texas and by a grant from the US Department of Energy, Office of Basic Energy Sciences (DE-FG02-05ER15647). This study was supported in part by NIH grants MD001633 from NCMHD (R.S.D.), EY014227, AG010485, AG022550, and AG027956 (P.K.) as well as by The Garvey Texas Foundation and the Felix and Carmen Sabates Missouri Endowed Chair in Vision Research (P.K.). Instrument acquisition and method development at the Kansas Lipidomics Research Center were supported by NSF grants MCB 0455318 and DBI 0521587, K-INBRE (NIH Grant P20 RR16475 from the INBRE program of the National Center for Research Resources), and NSF EPSCoR grant EPS-0236913 with matching support from the State of Kansas through Kansas Technology Enterprise Corporation and Kansas State University.

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Copyright information

© AOCS 2010

Authors and Affiliations

  • Aruna Kilaru
    • 1
    • 4
  • Giorgis Isaac
    • 2
    • 5
  • Pamela Tamura
    • 2
  • David Baxter
    • 1
  • Scott R. Duncan
    • 3
  • Barney J. Venables
    • 1
  • Ruth Welti
    • 2
  • Peter Koulen
    • 1
    • 3
  • Kent D. Chapman
    • 1
  1. 1.Department of Biological Sciences, Center for Plant Lipid ResearchUniversity of North TexasDentonUSA
  2. 2.Division of Biology, Kansas Lipidomics Research CenterKansas State UniversityManhattanUSA
  3. 3.Departments of Basic Medical Science and Ophthalmology, School of MedicineUniversity of Missouri-Kansas CityKansas CityUSA
  4. 4.Department of Plant BiologyMichigan State UniversityEast LansingUSA
  5. 5.Pacific Northwest National LaboratoryRichlandUSA

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