Lipids

, Volume 45, Issue 9, pp 843–854 | Cite as

Potential of Magnetic Resonance Spectroscopy in Assessing the Effect of Fatty Acids on Inflammatory Bowel Disease in an Animal Model

  • Sonal Varma
  • Michael N. A. Eskin
  • Ranjana Bird
  • Brion Dolenko
  • Jayadev Raju
  • Omkar B. Ijare
  • Tedros Bezabeh
Original Article

Abstract

People with inflammatory bowel disease (IBD) are at risk for developing colorectal cancer, and this risk increases at a rate of 1% per year after 8–10 years of having the disease. Saturated and ω-6 polyunsaturated fatty acids (PUFAs) have been implicated in its causation. Conversely, ω-3 PUFAs may have the potential to confer therapeutic benefit. Since proton magnetic resonance spectroscopy (1H MRS) combined with pattern recognition methods could be a valuable adjunct to histology, the objective of this study was to analyze the potential of 1H MRS in assessing the effect of dietary fatty acids on colonic inflammation. Forty male Sprague-Dawley rats were administered one of the following dietary regimens for 2 weeks: low-fat corn oil (ω-6), high-fat corn oil (ω-6), high-fat flaxseed oil (ω-3) or high-fat beef tallow (saturated fatty acids). Half of the animals were fed 2% carrageenan to induce colonic inflammation similar to IBD. 1H MRS and histology were performed on ex vivo colonic samples, and the 1H MR spectra were analyzed using a statistical classification strategy (SCS). The histological and/or MRS studies revealed that different dietary fatty acids modulate colonic inflammation differently, with high-fat corn oil being the most inflammatory and high-fat flaxseed oil the least inflammatory. 1H MRS is capable of identifying the biochemical changes in the colonic tissue as a result of inflammation, and when combined with SCS, this technique accurately differentiated the inflamed colonic mucosa based on the severity of the inflammation. This indicates that MRS could serve as a valuable adjunct to histology in accurately assessing colonic inflammation. Our data also suggest that both the type and the amount of fatty acids in the diet are critical in modulating IBD.

Keywords

Beef tallow Corn oil Flaxseed oil Inflammatory bowel disease Proton magnetic resonance spectroscopy Statistical classification strategy 

Abbreviations

FID

Free induction decay

GA_ORS

Genetic-algorithm-based optimal region selection

GC

Gas chromatography

H&E

Hematoxylin and eosin

HFB

High-fat beef tallow

HFC

High-fat corn oil

HFF

High-fat flaxseed oil

HPLC

High-performance liquid chromatography

HR MAS

High-resolution magic angle spinning

IBD

Inflammatory bowel disease

LDA

Linear discriminant analysis

LFC

Low-fat corn oil

LT

Leukotriene

MRS

Magnetic resonance spectroscopy

PAF

Platelet activating factor

PBS/D2O

Phosphate-buffered saline in deuterium oxide

PUFA

Polyunsaturated fatty acids

SCS

Statistical classification strategy

TSP

3-Trimethylsilylpropionic acid-d4 sodium salt

List of symbols

Gamma

γ

Omega

ω

Proton

1H

Notes

Acknowledgements

We would like to thank Saro Bascaramurty, who was the key resource for tissue sectioning and staining for histological assessment. We thank the Natural Sciences and Engineering Research Council of Canada (NSERC) for the strategic project grant to R.P. Bird, M. Eskin and T. Bezabeh.

Conflict of interest

There are no conflicts of interest to report.

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Copyright information

© Her Majesty the Queen in Right of Canada 2010

Authors and Affiliations

  • Sonal Varma
    • 1
    • 2
    • 5
  • Michael N. A. Eskin
    • 2
  • Ranjana Bird
    • 3
  • Brion Dolenko
    • 1
  • Jayadev Raju
    • 4
  • Omkar B. Ijare
    • 1
  • Tedros Bezabeh
    • 1
    • 2
  1. 1.National Research Council Institute for BiodiagnosticsWinnipegCanada
  2. 2.Department of Human Nutritional SciencesUniversity of ManitobaWinnipegCanada
  3. 3.Department of Biological SciencesUniversity of WindsorWindsorCanada
  4. 4.Department of BiologyUniversity of WaterlooWaterlooCanada
  5. 5.Department of Pathology and Molecular MedicineQueen’s UniversityKingstonCanada

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