Lipids

, Volume 42, Issue 8, pp 699–706

The Effects of Omega-3 Polyunsaturated Fatty Acids on TNF-α and IL-10 Secretion by Murine Peritoneal Cells In Vitro

  • Ingibjorg H. Skuladottir
  • Dagbjort H. Petursdottir
  • Ingibjorg Hardardottir
Original Article

DOI: 10.1007/s11745-007-3081-1

Cite this article as:
Skuladottir, I.H., Petursdottir, D.H. & Hardardottir, I. Lipids (2007) 42: 699. doi:10.1007/s11745-007-3081-1

Abstract

Omega-3 polyunsaturated fatty acids (PUFA) affect immune response, partly by affecting cytokine secretion. Omega-3 PUFA decrease tumor necrosis factor (TNF)-α secretion by RAW 264.7 macrophages but increase TNF-α secretion by primary elicited peritoneal macrophages in vitro. In this study, the effects of omega-3 and omega-6 PUFA on lipopolysaccharide induced TNF-α and interleukin (IL)-10 secretion by murine primary resident and elicited peritoneal macrophages and by RAW 264.7 macrophages, were examined in vitro using an enzyme-linked immunosorbent assay. In addition, the effects of dietary omega-3 PUFA on the number of cells secreting these cytokines were examined with enzyme-linked immunospot assay. All cell types secreted more TNF-α but similar amounts of IL-10 when incubated with the omega-3 PUFA, eicosapentaenoic acid or docosahexaenoic acid, compared with that when incubated with the omega-6 PUFA, linoleic acid or arachidonic acid. Dietary fish oil did not affect the number of TNF-α secreting resident peritoneal macrophages but decreased the number of macrophages secreting IL-10 ex vivo. These results show that dietary omega-3 PUFA and omega-3 PUFA added to cells in vitro increase TNF-α secretion by resident peritoneal macrophages, probably by a direct effect on the cells. In contrast, omega-3 PUFA did not affect IL-10 secretion by the cells but decreased the number of cells secreting IL-10 ex vivo, possibly by affecting cell recruitment, maturation or proliferation.

Keywords

Omega-3 fatty acids Macrophages TNF-α IL-10 

Copyright information

© AOCS 2007

Authors and Affiliations

  • Ingibjorg H. Skuladottir
    • 1
  • Dagbjort H. Petursdottir
    • 1
  • Ingibjorg Hardardottir
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Faculty of MedicineUniversity of IcelandReykjavikIceland

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