, Volume 42, Issue 7, pp 679–685 | Cite as

Classification of Adipose Tissue Species using Raman Spectroscopy

  • J. Renwick Beattie
  • Steven E. J. BellEmail author
  • Claus Borggaard
  • Anna M. Fearon
  • Bruce W. Moss
Original Article


In this study multivariate analysis of Raman spectra has been used to classify adipose tissue from four different species (chicken, beef, lamb and pork). The adipose samples were dissected from the carcass and their spectra recorded without further preparation. 102 samples were used to create and compare a range of statistical models, which were then tested on 153 independent samples. Of the classical multivariate methods employed, Partial Least Squares Discriminant Analysis (PLSDA) performed best with 99.6% correct classification of species in the test set compared with 96.7% for Principal Component Linear Discrimination Analysis (PCLDA). Kohenen and Feed-forward artificial neural networks compared well with the PLSDA, giving 98.4 and 99.2% correct classification, respectively.


Raman spectroscopy Gas chromatography Classification Speciation Adipose Fat Oil Lipid Fatty acid Triglyceride FAME 



Principal components analysis


Partial least squares discriminant analysis


Linear discriminant analysis


Gas chromatography


Fatty acid methyl ester


Polyunsaturated fatty acid


Monounsaturated fatty acid


Artificial neural network


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Copyright information

© AOCS 2007

Authors and Affiliations

  • J. Renwick Beattie
    • 1
  • Steven E. J. Bell
    • 1
    Email author
  • Claus Borggaard
    • 2
  • Anna M. Fearon
    • 3
  • Bruce W. Moss
    • 3
  1. 1.School of Chemistry and Chemical EngineeringQueen’s UniversityBelfastNorthern Ireland
  2. 2.Danish Meat Research InstituteRoskildeDenmark
  3. 3.School of Agriculture and Food ScienceQueen’s UniversityBelfastNorthern Ireland

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