Lipids

, Volume 42, Issue 6, pp 499–508 | Cite as

Identification and Characterization of a Novel Bovine Stearoyl-CoA Desaturase Isoform with Homology to Human SCD5

Original Article

Abstract

Stearoyl-CoA desaturase (SCD) is an enzyme responsible for the production of cis-9, trans-11 conjugated linoleic acid in ruminant fats, and for the synthesis of palmitoleoyl-CoA and oleoyl-CoA. To date, only one SCD isoform has been described in ruminant species, although multiple isoforms have been found in many other mammalian species. In this paper, we describe for the first time a second SCD isoform in cattle, which appears to be an ortholog of human SCD5 rather than a homolog of bovine SCD1 or any of the described murine SCD isoforms. As described in other SCD proteins, the predicted amino acid sequence of bovine SCD5 includes four transmembrane domains and three conserved histidine motifs. The amino-terminus of the predicted protein sequence of SCD5 lacks the PEST sequences typically found in SCD1 homologs, which are thought to target proteins for rapid degradation. Similar to human SCD5, the bovine SCD5 gene is organized into five exons and four introns, and is highly expressed in the brain. In other tissues examined, mRNA expression of SCD5 was minimal. Furthermore, the expression levels of SCD5 between brain gray and white matter are not different. This is the first description of a homolog of human SCD5 in a non-primate species.

Keywords

Conjugated linoleic acid Ruminants Genomic organization Expression profile 

Abbreviations

bp

Base pair(s)

cDNA

DNA complementary to RNA

CLA

Conjugated linoleic acid

kb

Kilobase(s) or 1000 bp

PCR

Polymerase chain reaction

RACE

Rapid amplification of cDNA ends

RT

Reverse transcription

SCD

Stearoyl-CoA desaturase

UTR

Untranslated region

References

  1. 1.
    Ip MM, Masso-Welch PA, Ip C (2003) Prevention of mammary cancer with conjugated linoleic acid: role of the stroma and the epithelium. J Mammary Gland Biol Neoplasia 8:103–118PubMedCrossRefGoogle Scholar
  2. 2.
    Brown JM, McIntosh MK (2003) Conjugated linoleic acid in humans: regulation of adiposity and insulin sensitivity. J Nutr 133:3041–3046PubMedGoogle Scholar
  3. 3.
    Corl BA, Barbano DM, Bauman DE, Ip C (2003) cis-9, trans-11 CLA derived endogenously from trans-11 18:1 reduces cancer risk in rats. J Nutr 133:2893–2900PubMedGoogle Scholar
  4. 4.
    Lock AL, Corl BA, Barbano DM, Bauman DE, Ip C (2004) The anticarcinogenic effect of trans-11 18:1 is dependent on its conversion to cis-9, trans-11 CLA by delta9-desaturase in rats. J Nutr 134:2698–2704PubMedGoogle Scholar
  5. 5.
    Lock AL, Horne CA, Bauman DE, Salter AM (2005) Butter naturally enriched in conjugated linoleic acid and vaccenic acid alters tissue fatty acids and improves the plasma lipoprotein profile in cholesterol-fed hamsters. J Nutr 135:1934–1939PubMedGoogle Scholar
  6. 6.
    Corl BA, Baumgard LH, Dwyer DA, Griinari JM, Phillips BS, Bauman DE (2001) The role of delta(9)-desaturase in the production of cis-9, trans-11 CLA. J Nutr Biochem 12:622–630PubMedCrossRefGoogle Scholar
  7. 7.
    Kay JK, Mackle TR, Auldist MJ, Thomson NA, Bauman DE (2004) Endogenous synthesis of cis-9, trans-11 conjugated linoleic acid in dairy cows fed fresh pasture. J Dairy Sci 87:369–378PubMedGoogle Scholar
  8. 8.
    Mosley EE, McGuire MK, Williams JE, McGuire MA (2006) cis-9, trans-11 conjugated linoleic acid is synthesized from vaccenic acid in lactating women. J Nutr 136:2297–2301PubMedGoogle Scholar
  9. 9.
    Santora JE, Palmquist DL, Roehrig KL (2000) trans-Vaccenic acid is desaturated to conjugated linoleic acid in mice. J Nutr 130:208–215PubMedGoogle Scholar
  10. 10.
    Enoch HG, Catala A, Strittmatter P (1976) Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme–substrate interactions, and the function of lipid. J Biol Chem 251:5095–5103PubMedGoogle Scholar
  11. 11.
    Kaestner KH, Ntambi JM, Kelly TJ Jr, Lane MD (1989) Differentiation-induced gene expression in 3T3-L1 preadipocytes. A second differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem 264:14755–14761PubMedGoogle Scholar
  12. 12.
    Miyazaki M, Jacobson MJ, Man WC, Cohen P, Asilmaz E, Friedman JM, Ntambi JM (2003) Identification and characterization of murine SCD4, a novel heart-specific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. J Biol Chem 278:33904–33911PubMedCrossRefGoogle Scholar
  13. 13.
    Ntambi JM, Buhrow SA, Kaestner KH, Christy RJ, Sibley E, Kelly TJ Jr, Lane MD (1988) Differentiation-induced gene expression in 3T3-L1 preadipocytes. Characterization of a differentially expressed gene encoding stearoyl-CoA desaturase. J Biol Chem 263:17291–17300PubMedGoogle Scholar
  14. 14.
    Zheng Y, Prouty SM, Harmon A, Sundberg JP, Stenn KS, Parimoo S (2001) Scd3––a novel gene of the stearoyl-CoA desaturase family with restricted expression in skin. Genomics 71:182–191PubMedCrossRefGoogle Scholar
  15. 15.
    Wang J, Yu L, Schmidt RE, Su C, Huang X, Gould K, Cao G (2005) Characterization of HSCD5, a novel human stearoyl-CoA desaturase unique to primates. Biochem Biophys Res Commun 332:735–742PubMedCrossRefGoogle Scholar
  16. 16.
    Zhang L, Ge L, Parimoo S, Stenn K, Prouty SM (1999) Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J 340(Pt 1):255–264PubMedCrossRefGoogle Scholar
  17. 17.
    Miyazaki M, Bruggink SM, Ntambi JM (2006) Identification of mouse palmitoyl-coenzyme A Delta9-desaturase. J Lipid Res 47:700–704PubMedCrossRefGoogle Scholar
  18. 18.
    Bernard L, Leroux C, Hayes H, Gautier M, Chilliard Y, Martin P (2001) Characterization of the caprine stearoyl-CoA desaturase gene and its mRNA showing an unusually long 3′-UTR sequence arising from a single exon. Gene 281:53–61PubMedCrossRefGoogle Scholar
  19. 19.
    Ward RJ, Travers MT, Richards SE, Vernon RG, Salter AM, Buttery PJ, Barber MC (1998) Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochim Biophys Acta 1391:145–156PubMedGoogle Scholar
  20. 20.
    Chung M, Ha S, Jeong S, Bok J, Cho K, Baik M, Choi Y (2000) Cloning and characterization of bovine stearoyl CoA desaturasel cDNA from adipose tissues. Biosci Biotechnol Biochem 64:1526–1530PubMedCrossRefGoogle Scholar
  21. 21.
    Campbell EM, Gallagher DS, Davis SK, Taylor JF, Smith SB (2001) Rapid communication: mapping of the bovine stearoyl-coenzyme A desaturase (SCD) gene to BTA26. J Anim Sci 79:1954–1955PubMedGoogle Scholar
  22. 22.
    Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402–408PubMedCrossRefGoogle Scholar
  23. 23.
    Shanklin J, Whittle E, Fox BG (1994) Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry 33:12787–12794PubMedCrossRefGoogle Scholar
  24. 24.
    Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132PubMedCrossRefGoogle Scholar
  25. 25.
    Man WC, Miyazaki M, Chu K, Ntambi JM (2006) Membrane topology of mouse stearoyl-CoA desaturase 1. J Biol Chem 281:1251–1260PubMedCrossRefGoogle Scholar
  26. 26.
    Zhang S, Yang Y, Shi Y (2005) Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J 388:135–142PubMedCrossRefGoogle Scholar
  27. 27.
    Ren J, Knorr C, Huang L, Brenig B (2004) Isolation and molecular characterization of the porcine stearoyl-CoA desaturase gene. Gene 340:19–30PubMedCrossRefGoogle Scholar
  28. 28.
    Svennerholm L (1968) Distribution and fatty acid composition of phosphoglycerides in normal human brain. J Lipid Res 9:570–579PubMedGoogle Scholar
  29. 29.
    Oshino N, Sato R (1972) The dietary control of the microsomal stearyl CoA desaturation enzyme system in rat liver. Arch Biochem Biophys 149:369–377PubMedCrossRefGoogle Scholar
  30. 30.
    Heinemann FS, Ozols J (1998) Degradation of stearoyl-coenzyme A desaturase: endoproteolytic cleavage by an integral membrane protease. Mol Biol Cell 9:3445–3453PubMedGoogle Scholar
  31. 31.
    Rechsteiner M, Rogers SW (1996) PEST sequences and regulation by proteolysis. Trends Biochem Sci 21:267–271PubMedCrossRefGoogle Scholar
  32. 32.
    Mziaut H, Korza G, Ozols J (2000) The N terminus of microsomal delta 9 stearoyl-CoA desaturase contains the sequence determinant for its rapid degradation. Proc Natl Acad Sci USA 97:8883–8888PubMedCrossRefGoogle Scholar
  33. 33.
    Kato H, Sakaki K, Mihara K (2006) Ubiquitin-proteasome-dependent degradation of mammalian ER stearoyl-CoA desaturase. J Cell Sci 119:2342–2353PubMedCrossRefGoogle Scholar
  34. 34.
    Mziaut H, Korza G, Benraiss A, Ozols J (2002) Selective mutagenesis of lysyl residues leads to a stable and active form of delta 9 stearoyl-CoA desaturase. Biochim Biophys Acta 1583:45–52PubMedGoogle Scholar
  35. 35.
    Jones BH, Maher MA, Banz WJ, Zemel MB, Whelan J, Smith PJ, Moustaid N (1996) Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids. Am J Physiol 271:E44–49PubMedGoogle Scholar
  36. 36.
    Heinemann FS, Ozols J (2003) Stearoyl-CoA desaturase, a short-lived protein of endoplasmic reticulum with multiple control mechanisms. Prostaglandins Leukot Essent Fatty Acids 68:123–133PubMedCrossRefGoogle Scholar
  37. 37.
    Peterson DG, Kelsey JA, Bauman DE (2002) Analysis of variation in cis-9, trans-11 conjugated linoleic acid (CLA) in milk fat of dairy cows. J Dairy Sci 85:2164–2172PubMedGoogle Scholar
  38. 38.
    Kelsey JA, Corl BA, Collier RJ, Bauman DE (2003) The effect of breed, parity, and stage of lactation on conjugated linoleic acid (CLA) in milk fat from dairy cows. J Dairy Sci 86:2588–2597PubMedCrossRefGoogle Scholar
  39. 39.
    McDonald TM, Kinsella JE (1973) Stearyl-CoA desaturase of bovine mammary microsomes. Arch Biochem Biophys 156:223–231PubMedCrossRefGoogle Scholar
  40. 40.
    Bourre JM, Dumont OL, Clement ME, Durand GA (1997) Endogenous synthesis cannot compensate for absence of dietary oleic acid in rats. J Nutr 127:488–493PubMedGoogle Scholar
  41. 41.
    Edmond J, Higa TA, Korsak RA, Bergner EA, Lee WN (1998) Fatty acid transport and utilization for the developing brain. J Neurochem 70:1227–1234PubMedCrossRefGoogle Scholar
  42. 42.
    Garbay B, Boiron-Sargueil F, Shy M, Chbihi T, Jiang H, Kamholz J, Cassagne C (1998) Regulation of oleoyl-CoA synthesis in the peripheral nervous system: demonstration of a link with myelin synthesis. J Neurochem 71:1719–1726PubMedCrossRefGoogle Scholar
  43. 43.
    Granda B, Tabernero A, Tello V, Medina JM (2003) Oleic acid induces GAP-43 expression through a protein kinase C-mediated mechanism that is independent of NGF but synergistic with NT-3 and NT-4/5. Brain Res 988:1–8PubMedCrossRefGoogle Scholar
  44. 44.
    Velasco A, Tabernero A, Medina JM (2003) Role of oleic acid as a neurotrophic factor is supported in vivo by the expression of GAP-43 subsequent to the activation of SREBP-1 and the up-regulation of stearoyl-CoA desaturase during postnatal development of the brain. Brain Res 977:103–111PubMedCrossRefGoogle Scholar

Copyright information

© AOCS 2007

Authors and Affiliations

  1. 1.Department of Dairy ScienceVirginia Polytechnic Institute and State UniversityBlacksburgUSA
  2. 2.BlacksburgUSA

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