Lipids

, Volume 41, Issue 9, pp 865–876 | Cite as

Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of atlantic salmon (Salmo salar L.) grown at elevated temperature

  • Matthew R. Miller
  • Peter D Nichols
  • Julia Barnes
  • Noel W. Davies
  • Evan J. Peacock
  • Chris G. Carter
Articles

Abstract

Regiospecific and traditional analysis, of both storage and membrane lipids, was performed on gill, white muscle, and red muscle samples taken from Atlantic salmon (Salmo salar) to gauge the effect of elevated water temperature. The fish, fed a commercial diet, were held at an elevated water temperature of 19°C. Total n-3 PUFA, total PUFA, and n-3/n-6 and unsaturated/saturated fatty acid (UFA/SFA) ratios in the FA profile of the total lipid extract in the white muscle were fairly low compared with fish grown at 15°C. Adaptation of structural and storage lipids at elevated temperatures was shown by a significant (P<0.01) reduction in PUFA especially in the percentage of EPA (6–8%). Further adaptation was indicated by the percentages of SFA, which were significantly (P<0.05) higher in gill (56%) and white muscle (58%) polar lipid fractions and coincided with lower percentages of n-3, n-6, and total PUFA. The regiospecific profiles indicated a high affinity of DHA to the sn-2 position in both the TAG (61–68%) and polar lipid (35–60%) fractions. The combination of detailed regiospecific and lipid analyses demonstrated adaptation of cell membrane structure in Atlantic salmon grown at an elevated water temperature.

Abbreviations

CDCl3

detiterated chloroform

DPA

docosapentaenoic acid

ESI-RP-LCMS

electrospray ionization reversed-phase liquid chromatography-MS

LC

long-chain

MUFA

monounsaturated FA

PL

polar lipid

PLFA

polar lipid FA

SFA

saturated FA

TLE

total lipid extract

tr

trace amounts

UPL

undetermined PL

UFA

unsaturated FA

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Copyright information

© AOCS Press 2006

Authors and Affiliations

  • Matthew R. Miller
    • 1
    • 2
  • Peter D Nichols
    • 2
  • Julia Barnes
    • 1
  • Noel W. Davies
    • 3
  • Evan J. Peacock
    • 3
  • Chris G. Carter
    • 1
  1. 1.School of Aquaculture, Tasmanian Aquaculture and Fisheries InstituteUniversity of Tasmania (UTAS)LauncestonAustralia
  2. 2.Food Futures Flagship, Marine and Atmospheric ResearchCSIROHobartAustralia
  3. 3.Central Science LaboratoryUTASHobartAustralia

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