Lipids

, Volume 37, Issue 10, pp 981–989 | Cite as

Comparative effect of fenofibrate on hepatic desaturases in wild-type and peroxisome proliferator-activated receptor α-deficient mice

  • Hervé Guillou
  • Pascal Martin
  • Sophie Jan
  • Sabine D’Andrea
  • Alain Roulet
  • Daniel Catheline
  • Vincent Rioux
  • Thierry Pineau
  • Philippe Legrand
Articles

Abstract

In this study is presented the effect of fenofibrate, a prototypical peroxisome proliferator of the fibrate class, on wild-type and peroxisome proliferator-activated receptor α (PPARα)-/-mouse liver FA profile, desaturase mRNA levels, and activities. We established that, following peroxisome proliferator exposure, the hepatic FA profile was greatly modified. These modifications in hepatic FA content required the expression of PPARα, as they are suppressed in transgenic mice deficient in this nuclear receptor. Following peroxisome proliferator exposure, Δ6- and Δ5-desaturase mRNA levels and activities were increased in wild-type but not in PPARα-deficient mouse liver. These results suggest the involvement of PPARα in the control of hepatic Δ6- and Δ5-desaturases in mice. Their roles in minimizing long-chain PUFA depletion in the liver during peroxisome proliferator exposure are discussed.

Abbreviations

AOX

peroxisomal acyl-CoA oxidase

CYP4A10

cytochrome P450 4A10

PP

peroxisome proliferator

PPARα

peroxisome proliferator-activated receptor α

PPRE

peroxisome proliferator responsive element

SCD

stearoyl-CoA desaturase

TBS

Tris-buffered saline

W-T

wild-type

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Copyright information

© AOCS Press 2002

Authors and Affiliations

  • Hervé Guillou
    • 1
  • Pascal Martin
    • 2
  • Sophie Jan
    • 1
    • 3
  • Sabine D’Andrea
    • 1
  • Alain Roulet
    • 2
  • Daniel Catheline
    • 1
  • Vincent Rioux
    • 1
  • Thierry Pineau
    • 2
  • Philippe Legrand
    • 1
  1. 1.Laboratoire de Biochimie ENSAR-INRARennesFrance
  2. 2.Laboratoire de Pharmacologie et ToxicologieINRAToulouseFrance
  3. 3.Université du MaineLe MansFrance

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