, Volume 40, Issue 11, pp 1171–1177 | Cite as

Inhibition of 12- and 15-lipoxygenase activities and protection of human and tilapia low density lipoprotein oxidation by I-Tiao-Gung (Glycine tomentella)



I-Tiao-Gung, Glycine tomentella, has been used extensively as a traditional herbal medicine to relieve physical pain, but its bioactivity has not been studied systematically. Ninety-five percent ethanol extracts of G. tomentella (GT-E) showed antioxidant activity in human plasma by prolonging the lag phase (+Tlag) of Cu2+-induced, LDL oxidation and were dose dependent. The+Tlag of LDL combined with 3.2 μg/mL GT-E was similar to that with 2.0 μM (ca. 0.5 μg/mL) Trolox. A similar inhibitory effect was found toward tilapia plasma LDL. In addition, GT-E inhibited tilapia thrombocyte (nucleated platelet), 5-, 12-, and 15-lipoxygenase (LOX). The IC50 values were 0.43, 0.72, and 0.42 μg/mL, respectively, whereas the IC50 values for nordihydroguaiaretic acid (NDGA) on 5-, 12-, and 15-LOX were 2.3, 1.6, and 1.7 μg/mL, respectively. The IC50 value for cyclooxygenase-2 (COX-2) inhibition by GT-E was 42.0 μg/mL, whereas the IC50 value by indomethacin as a positive control was 0.61 μg/mL. The prevention of LDL oxidation and the dual inhibition of LOX and COX-2 are indicative of the possible roles of I-Tiao-Gung in antiatherosclerosis and anti-inflammation.





Glycine tomentella Hayata


95% ethanol extracts of Glycine tomentella


human recombinant COX-2


hydroxyl eicosatetraenoic acid




leukotrine B4


nordihydroguaiaretic acid


lag phase of LDL oxidation


6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid


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Copyright information

© AOCS Press 2005

Authors and Affiliations

  • Tsui-Yao Chen
    • 1
    • 2
  • Ming-Shi Shiao
    • 3
  • Bonnie Sun Pan
    • 1
  1. 1.Food Science DepartmentNational Taiwan Ocean UniversityKeelungRepublic of China Taiwan
  2. 2.Food Science DepartmentNational I-Lan UniversityI-LanTaiwan, Republic of China
  3. 3.Department of Medical Research and EducationVeterans General Hospital-TaipeiTaiwan, Republic of China

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