Lipids

, Volume 40, Issue 5, pp 523–528

Simultaneous quantification of free fatty acids, free sterols, squalene, and acylglycerol molecular species in palm oil by high-temperature gas chromatography-flame ionization detection

  • Harrison Lik Nang Lau
  • Chiew Wei Puah
  • Yuen May Choo
  • Ah Ngan Ma
  • Cheng Hock Chuah
Methods

DOI: 10.1007/s11745-005-1413-1

Cite this article as:
Nang Lau, H.L., Puah, C.W., Choo, Y.M. et al. Lipids (2005) 40: 523. doi:10.1007/s11745-005-1413-1

Abstract

This paper discusses a rapid GC-FID technique for the simultaneous quantitative analysis of FFA, MAG, DAG, TAG, sterols, and squalene in vegetable oils, with special reference to palm oil. The FFA content determined had a lower SE compared with a conventional titrimetric method. Squalene and individual sterols, consisting of β-sitosterol, stigmasterol, campesterol, and cholesterol, were accurately quantified without any losses. This was achieved through elimination of tedious conventional sample pretreatments, such as saponification and preparative TLC. With this technique, the separation of individual MAG, consisting of 16∶0, 18∶0, and 18∶1 FA, and the DAG species, consisting of the 1,2(2,3)- and 1,3-positions, was sufficient to enable their quantification. This technique enabled the TAG to be determined according to their carbon numbers in the range of C44 to C56. Comparisons were made with conventional methods, and the results were in good agreement with those reported in the literature.

Abbreviations

BSTFA

N,O-bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane

CPO

crude palm oil

MPOB

Malaysian Palm Oil Board

PORIM

Palm Oil Research Institute of Malaysia

SFC

supercritical fluid chromatography

Copyright information

© AOCS Press 2005

Authors and Affiliations

  • Harrison Lik Nang Lau
    • 2
    • 1
  • Chiew Wei Puah
    • 2
    • 1
  • Yuen May Choo
    • 2
  • Ah Ngan Ma
    • 2
  • Cheng Hock Chuah
    • 1
  1. 1.University of MalayaKuala LumpurMalaysia
  2. 2.Malaysian Palm Oil Board, No. 6Persiaran InstitusiKajang, SelangorMalaysia

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