Lipids

, 38:1221

Intramuscular injection of antigens and adjuvant preferentially decreases 18∶2n−6 and 18∶3n−3 in pig neck muscle

  • Richard P. Bazinet
  • Holly Douglas
  • Ewen G. McMillan
  • Bruce N. Wilkie
  • Stephen C. Cunnane
Articles

Abstract

Linoleic (18∶2n−6) and α-linolenic acids (18∶3n−3) have many important physiological functions including immunomodulation. We tested how immunization influences the metabolism of 18∶2n−6 and 18∶3n−3 in the neck muscle of pigs. At 35 d old, pigs received either an intramuscular neck injection containing hen egg white lysozyme (HEWL), killed Mycobacterium tuberculosis, and Freund’s complete adjuvant (immunized) or PBS (control). At 49 d old, immunized pigs received a booster injection of HFWI and Freund’s incomplete adjuvant, and the control pigs received PBS into the neck. At 56 d old, all pigs received an intradermal injection of Mycobacterium bovis into the hind leg to induce a delayed-type hypersensitivity (DTH) reaction. At 57 d old, immunized pigs had a twofold increase in serum haptoglobin, a 10-fold increase in antibodies to HEWL, and the skinfold at the DTH reaction site was 10 times thicker than the controls. Both 18∶2n−6 and 18∶3n−3 (% composition) were approximately 25% lower in muscle IG, 40% lower in FFA, 50% lower in phospholipids, but not different in cholesteryl esters of the neck muscle of immunized pigs. The antigens in this model induce an increased response in the innate (haptoglobin), humoral (antibodies), and cellular (DTH) immune systems as well as a preferential decrease of 18∶2n−6 and 18∶3n−3 in the inflamed neck muscle. It appears that 18∶2n−6 and 18∶3n−3 are preferentially metabolized (possibly β-oxidized) in response to antigens.

Abbreviations

18∶2n−6

linoleic acid

18∶3n−3

α-linolenic acid

20∶4n−6

arachidonic acid

22∶6n−3

DHA

CMI

cell-mediated immune/immunity

DTH

delayed-type hypersensitivity

FCA

Freund’s complete adjuvant

HEWL

hen egg white lysozyme

HSL

hormone-sensitive lipase

i.d.

intradermal

i.m.

intramuscular

PPD

purified protein derivative

Copyright information

© AOCS Press 2003

Authors and Affiliations

  • Richard P. Bazinet
    • 1
  • Holly Douglas
    • 1
  • Ewen G. McMillan
    • 2
  • Bruce N. Wilkie
    • 3
  • Stephen C. Cunnane
    • 1
  1. 1.Department of Nutritional Sciences, Faculty of MedicineUniversity of TorontoTorontoCanada
  2. 2.Maple Leaf Foods AgresearchGuelphCanada
  3. 3.Department of PathobiologyUniversity of GuelphGuelphCanada
  4. 4.Brain Physiology and Metabolism Section, National Institute on AgingNational Institutes of HealthBethesda

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