, Volume 38, Issue 11, pp 1115–1118

Serum and aortic levels of phytosterols in rabbits fed sitosterol or sitostanol ester preparations

  • David Kritchevsky
  • Shirley A. Tepper
  • Susanne K. Czarnecki
  • Brian Wolfe
  • Kenneth D. R. Setchell


Campesterol is present in all the phytosterol-containing dietary hypocholesterolemic agents in current use. Campesterol is absorbed more efficiently than sitosterol, and the question of its possible atherogenicity has been raised. To test this possibility, rabbits were fed either a semipurified, cholesterol-free diet that has been shown to be atherogenic for this species or the same diet augmented with 0.5 g of phytosterol-rich diet preparations (spreads) containing either sitosterol or sitostanol. The diets contained 295 mg phytosterol per 100 g. After 60 d, serum cholesterol levels in the two phytosterol groups were 78±4 mg/dL (sitosterol) and 76±4 mg/dL (sitostanol), respectively. The serum cholesterol level of rabbits fed the control diet was 105±8 mg/dL. Serum campesterol (μg/mL) levels were higher than sitosterol or sitostanol levels in all groups. Aortic phytosterols were present in nanogram quantities compared to cholesterol, which was present in microgram quantities. The ratio of campesterol/sitosterol/sitostanol in the aortas was: control, 1.00∶0.43∶0.02; sitosterol, 1∶00∶0.32∶0.01; sitostanol, 1∶00∶0.34∶0.11. Aortic campesterol was present at 4% the concentration of aortic cholesterol, sitosterol at 1.4%, and sitostanol at 0.14%. Aortic lesions were not present in any of the animals.


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  1. 1.
    Peterson, D.W. (1951) Effect of Soybean Sterols in the Diet on Plasma and Liver Cholesterol in Chickens, Proc. Soc. Exp. Biol. Med. 78, 143–147.PubMedGoogle Scholar
  2. 2.
    Pollak, O.J. (1953) Reduction of Blood Cholesterol in Man, Circulation 7, 702–706.PubMedGoogle Scholar
  3. 3.
    Pollak, O.J., and Kritchevsky, D. (1981) Sitosterol, Karger, Basel.Google Scholar
  4. 4.
    Shipley, R.E., Pfeiffer, R.R., Marsh, M.M., and Anderson, R.C. (1958) Sitosterol Feeding: Chronic Animal and Clinical Toxicology and Tissue Analysis, Circ. Res. 6, 373–382.PubMedGoogle Scholar
  5. 5.
    Lees, R.S., and Lees, A.M. (1976) Effects of Sitosterol Therapy on Plasma Lipid and Lipoprotein Concentration, in Lipoprotein Metabolism (Greten, H., ed.), pp. 119–124, Springer Verlag, Berlin.Google Scholar
  6. 6.
    Lees, A.M., Mok, H.Y.I., Lees, R.S., McCluskey, M.A., and Grundy, S.M. (1977) Plant Sterols as Cholesterol-Lowering Agents: Clinical Trials in Patients with Hypercholesterolemia and Studies of Sterol Balance, Atherosclerosis 28, 325–338.PubMedCrossRefGoogle Scholar
  7. 7.
    Miettinen, T.A., Puska, P., Gylling, H., Vanhanen, H., and Vartainen, E. (1995) Reduction of Serum Cholesterol with a Sitostanol-Ester Margarine in a Mildly Hypercholesterolemic Population, N. Engl. J. Med. 333, 1308–1312.PubMedCrossRefGoogle Scholar
  8. 8.
    Westrate, J.A., and Meijer, G.W. (1998) Plant Sterol Enhanced Margarines and Reduction of Plasma Total- and LDL-Cholesterol Concentrations in Normocholesterolemic and Mildly Hypercholesterolemic Subjects, Eur. J. Clin. Nutr. 52, 334–343.CrossRefGoogle Scholar
  9. 9.
    Duff, G.L., and McMillan, G.C. (1949) The Effect of Alloxan Diabetes on Experimental Atherosclerosis in Rabbits, J. Exp. Med. 89, 611–630.CrossRefPubMedGoogle Scholar
  10. 10.
    Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226, 497–509.PubMedGoogle Scholar
  11. 11.
    Kritchevsky, D., and Tepper, S.A. (1965) Factors Affecting Atherosclerosis in Rabbits Fed Cholesterol-Free Diets, Life Sci. 4, 1467–1471.PubMedCrossRefGoogle Scholar
  12. 12.
    Kritchevsky, D., and Tepper, S.A. (1968) Experimental Atherosclerosis in Rabbits Fed Cholesterol-Free Diets, J. Atheroscler. Res. 8, 357–369.PubMedCrossRefGoogle Scholar
  13. 13.
    Pollak, O.J. (1953) Successful Prevention of Experimental Hypercholesterolemia and Cholesterol Atherosclerosis in Rabbits, Circulation 7, 696–701.PubMedGoogle Scholar
  14. 14.
    Diller, E.R., Woods, B.L., and Harvey, O.A. (1958) Effect of β-Sitosterol on Regression of Hypercholesterosis and Atherosclerosis in Chickens, Proc. Soc. Exp. Biol. Med. 98, 813–817.PubMedGoogle Scholar
  15. 15.
    Hardegger, E., Ruzicka, L., and Tagmann, E. (1943) Untersuchungen über Organe Extracte. Zur Kenntnis der Unverseif-baren Lipoide aus Arteriosklerotischen Aorten, Helv. Chim. Acta 30, 2205–2221.CrossRefGoogle Scholar
  16. 16.
    Brooks, C.J.W., Steel, G., Gilbert, J.D., and Starland, W.A. (1971) Lipids of Human Atheroma. Part 4. Characterization of a New Group of Polar Sterol Esters from Human Atherosclerotic Plaques, Atherosclerosis 13, 223–237.PubMedCrossRefGoogle Scholar
  17. 17.
    Hodis, H.N., Crawford, D.W., and Sevanian, A. (1991) Cholesterol Feeding Increases Plasma and Aortic Tissue Cholesterol Oxide Levels in Parallel: Further Evidence for the Role of Cholesterol Oxidation in Atherosclerosis, Atherosclerosis 89, 117–126.PubMedCrossRefGoogle Scholar
  18. 18.
    Vaya, J., Aviram, M., Mahmood, S., Hayek, T., Grenader, E., Hoffman, A., and Milo, S. (2001) Selective Distribution of Oxysterols in Atherosclerotic Lesions and Human Plasma Lipoproteins, Free Rad. Res. 34, 485–497.CrossRefGoogle Scholar
  19. 19.
    Cook, R.P., Kliman, A., and Fieser, L.F. (1954) The Absorption and Metabolism of Cholesterol and Its Main Companions in the Rabbit—With Observations on the Atherogenic Nature of the Sterols, Arch. Biochem. 52, 439–450.PubMedCrossRefGoogle Scholar
  20. 20.
    Kritchevsky, D., Fumagalli, R., Cattabeni, F., and Tepper, S.A. (1970) Effect of Triparanol on Sterol Composition in Normal and Atherosclerotic Rabbit Aorta, Rev. Farmacol. Terapia 1, 455–463.Google Scholar

Copyright information

© AOCS Press 2003

Authors and Affiliations

  • David Kritchevsky
    • 3
  • Shirley A. Tepper
    • 3
  • Susanne K. Czarnecki
    • 1
  • Brian Wolfe
    • 2
  • Kenneth D. R. Setchell
    • 2
  1. 1.Department of ChemistryChestnut Hill CollegePhiladelphia
  2. 2.Department of PediatricsChildren's Hospital Medical CenterCincinnati
  3. 3.The Wistar InstitutePhiladelphia

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